FastQCFastQC Report
Thu 9 Feb 2017
SRR2727526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668496
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.14875672461905812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18220.10920014192422396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16900.10128882538525713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA358.87108E-426.4285722
TACGCCG450.00382652220.5555555
CTTATAC10850.020.29032337
CGTATAC656.904517E-519.9230773
ATAATAC1258.58563E-917.763
CGTCTAG650.00158033817.0769231
GTATACT1107.812796E-716.8181824
TCTTATA19750.015.73670837
GGACCGT1353.9749648E-715.0740756
CCCTATA1152.2109167E-514.4782612
GTCCTAT1854.9021764E-914.01
CGTTACG3100.013.72580724
TACGGAT7550.013.72185527
TCGTTAC3950.013.5822823
ACGGATG7650.013.54248328
GTATTAT1652.596753E-713.4545451
TTATACT2202.8376235E-1013.4545454
GTATATA1951.0266376E-813.282051
GTCTAGT1409.564212E-613.2142861
CTCTATA1409.564212E-613.2142862
Produced by FastQC (version 0.11.5)