##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727526.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1668496
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23455435314199	33.0	31.0	34.0	30.0	34.0
2	32.41868485150699	34.0	31.0	34.0	31.0	34.0
3	32.51149897872096	34.0	31.0	34.0	31.0	34.0
4	36.07803075344502	37.0	35.0	37.0	35.0	37.0
5	35.85791395364448	37.0	35.0	37.0	35.0	37.0
6	35.87134281412722	37.0	35.0	37.0	35.0	37.0
7	35.84177486790499	37.0	35.0	37.0	35.0	37.0
8	35.82270380030878	37.0	35.0	37.0	35.0	37.0
9	37.48767213106894	39.0	37.0	39.0	35.0	39.0
10	37.42689883583778	39.0	37.0	39.0	35.0	39.0
11	37.50602278938637	39.0	37.0	39.0	35.0	39.0
12	37.472938502699435	39.0	37.0	39.0	35.0	39.0
13	37.513008721627145	39.0	37.0	39.0	35.0	39.0
14	38.772964993623	40.0	38.0	41.0	35.0	41.0
15	38.77144026716276	40.0	38.0	41.0	35.0	41.0
16	38.75359005955064	40.0	38.0	41.0	35.0	41.0
17	38.762765388709354	40.0	38.0	41.0	35.0	41.0
18	38.73691576126044	40.0	38.0	41.0	35.0	41.0
19	38.77675823016657	40.0	38.0	41.0	35.0	41.0
20	38.757391686884475	40.0	38.0	41.0	35.0	41.0
21	38.7290056434058	40.0	38.0	41.0	35.0	41.0
22	38.69572836854269	40.0	38.0	41.0	35.0	41.0
23	38.644555336063135	40.0	38.0	41.0	35.0	41.0
24	38.59602360449171	40.0	38.0	41.0	34.0	41.0
25	38.53059521868797	40.0	38.0	41.0	34.0	41.0
26	38.406551169436426	40.0	38.0	41.0	34.0	41.0
27	38.34356030820571	40.0	38.0	41.0	34.0	41.0
28	38.27171236850433	40.0	38.0	41.0	34.0	41.0
29	38.16646428879662	40.0	38.0	41.0	34.0	41.0
30	38.0988423106798	40.0	38.0	41.0	34.0	41.0
31	38.01944206039451	40.0	38.0	41.0	33.0	41.0
32	37.92139927215888	40.0	37.0	41.0	33.0	41.0
33	37.86536917079813	40.0	37.0	41.0	33.0	41.0
34	37.80740738964912	40.0	37.0	41.0	33.0	41.0
35	37.78677203900998	40.0	37.0	41.0	33.0	41.0
36	37.72432298309376	40.0	37.0	41.0	33.0	41.0
37	37.6897343475801	40.0	37.0	41.0	33.0	41.0
38	37.646326991494135	40.0	37.0	41.0	33.0	41.0
39	37.59123066522185	40.0	37.0	41.0	33.0	41.0
40	37.52298417257219	40.0	37.0	41.0	33.0	41.0
41	37.472219891447146	40.0	37.0	41.0	32.0	41.0
42	37.41766596983151	39.0	37.0	41.0	32.0	41.0
43	36.2988511809438	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	2.0
13	1.0
14	2.0
15	4.0
16	11.0
17	16.0
18	28.0
19	75.0
20	146.0
21	271.0
22	586.0
23	1043.0
24	1867.0
25	2967.0
26	4572.0
27	6922.0
28	9837.0
29	13936.0
30	18985.0
31	25512.0
32	34050.0
33	45343.0
34	63135.0
35	89479.0
36	136449.0
37	237467.0
38	504097.0
39	471688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05662105273252	17.342145261361132	12.447317823956427	27.153915861949923
2	18.469387999731495	19.932082546197293	35.383902628474985	26.214626825596227
3	20.23379139056971	21.75468206096988	29.329048436436167	28.68247811202424
4	15.538484958909102	14.81813561434969	33.0741578043939	36.569221622347314
5	16.34699753550503	34.915696531487036	33.06354944812574	15.673756484882192
6	36.89748132449823	32.98671378295183	14.581635197207547	15.534169695342392
7	30.59252164823889	29.524014441748736	20.403105551346844	19.480358358665526
8	27.622601432667505	34.49471859686808	19.099416480471035	18.78326348999338
9	28.046276407015657	14.342377806120002	17.93183801459518	39.679507772269154
10	17.18709544404062	26.221758997324535	32.41176484690404	24.179380711730804
11	37.32804873370988	20.86909408233523	20.295523633260135	21.507333550694756
12	22.495888512768385	23.684503888531946	29.30105915746876	24.518548441230905
13	32.08248626307765	18.90966475196824	23.11452949242911	25.893319492525002
14	23.35324747557081	19.339932490098867	24.497451597126993	32.809368437203325
15	26.691942923447225	26.29631716228268	21.31500465089518	25.69673526337492
16	25.608332294473584	25.00905006664685	23.697329810799665	25.6852878280799
17	25.228649034819384	25.03602046393878	24.349474017318588	25.38585648392325
18	25.413066618079995	24.681749311955198	24.89361676623738	25.01156730372743
19	26.401621580153623	24.612765029104054	24.70062859005955	24.28498480068277
20	25.638539139440553	24.13490952330722	24.702486550761886	25.524064786490346
21	25.196764031798697	24.936289928174833	24.999640394702773	24.867305645323693
22	25.994848054775076	24.28732223511474	23.99981779998274	25.71801191012744
23	25.435721751805218	24.295113683221295	24.497931070856627	25.771233494116856
24	25.19316797882644	24.23404071690912	24.65633720428458	25.916454099979862
25	25.74708000498653	23.823731072774525	24.320525790891917	26.108663131347033
26	25.37722595678983	24.26616545679462	24.802696560255463	25.553912026160084
27	25.834344223779983	24.581898907758845	24.333591450024453	25.250165418436726
28	25.563381632320365	25.006832500647292	23.89415377681457	25.535632090217774
29	25.248547194599208	24.940185652228113	24.82145596992741	24.98981118324527
30	25.914596139277528	24.51135633528639	24.25483788993201	25.31920963550407
31	25.467666689042108	24.295593156950932	24.713694249192088	25.52304590481487
32	24.888822028941036	24.62019687191339	24.63056549131673	25.860415607828845
33	25.071501519931722	24.177822422109493	24.652081874934073	26.098594183024716
34	25.494277481036814	24.30158657857136	25.230147390224488	24.973988550167338
35	25.37213154841246	24.091577084991513	24.94569960011891	25.590591766477118
36	25.058375926582983	23.9225026610792	25.363800692360066	25.65532071997775
37	24.41546158935952	23.518066570132625	25.889303900039316	26.177167940468543
38	24.861791697432896	23.141499889721043	25.867068305827523	26.12964010701854
39	24.069581227644537	23.244047333646588	26.802821223425166	25.88355021528371
40	24.830086497060826	22.730111429694766	26.98975604376636	25.450046029478045
41	24.076833267805256	22.994960731101543	27.092902829853955	25.83530317123925
42	23.44338853674207	23.195680421169723	27.512382409067808	25.848548633020396
43	23.19268371035951	22.826485649351273	27.351219301694464	26.62961133859476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	69.0
2	95.0
3	220.0
4	345.0
5	345.0
6	509.0
7	673.0
8	648.5
9	624.0
10	851.5
11	1079.0
12	1079.0
13	1873.5
14	2668.0
15	4092.0
16	5516.0
17	5059.5
18	4603.0
19	4603.0
20	5296.0
21	5989.0
22	5882.0
23	5775.0
24	7255.5
25	8736.0
26	8736.0
27	10893.5
28	13051.0
29	15715.5
30	18380.0
31	22758.0
32	27136.0
33	27136.0
34	32492.0
35	37848.0
36	44065.0
37	50282.0
38	58048.5
39	65815.0
40	65815.0
41	74291.0
42	82767.0
43	92233.0
44	101699.0
45	113092.0
46	124485.0
47	124485.0
48	136778.0
49	149071.0
50	156799.5
51	164528.0
52	166495.5
53	168463.0
54	168463.0
55	159402.0
56	150341.0
57	138871.5
58	127402.0
59	118298.5
60	109195.0
61	109195.0
62	95902.5
63	82610.0
64	69935.5
65	57261.0
66	47897.5
67	38534.0
68	38534.0
69	31658.5
70	24783.0
71	20279.0
72	15775.0
73	12787.0
74	9799.0
75	9799.0
76	7898.0
77	5997.0
78	4780.0
79	3563.0
80	2752.0
81	1941.0
82	1941.0
83	1433.0
84	925.0
85	680.5
86	436.0
87	309.5
88	183.0
89	183.0
90	117.5
91	52.0
92	35.0
93	18.0
94	13.0
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1668496.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.09525303735487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.5531490273342	57.576680781214975
2	8.517634832833581	11.600209984431809
3	2.712246410023016	5.540733167705238
4	1.2935398941465046	3.523357056232778
5	0.7670060249395766	2.611473467471809
6	0.49668063870974033	2.0292956263016877
7	0.34063113534585066	1.623675434764402
8	0.24521091902122627	1.3358159662618188
9	0.18028342139282927	1.1048800678366306
>10	0.8550318826838165	9.552178568887756
>50	0.02642521519224438	1.2261762737766015
>100	0.011015197567669511	1.4426424963003106
>500	7.048620368546188E-4	0.3307645550275648
>1k	4.405387730341367E-4	0.5021165537866298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2482	0.14875672461905812	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1822	0.10920014192422396	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1690	0.10128882538525713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1986843240858833E-4	0.0
3	0.0	0.0	0.0	1.1986843240858833E-4	0.0
4	0.0	0.0	0.0	1.1986843240858833E-4	0.0
5	0.0	0.0	0.0	2.3973686481717666E-4	0.0
6	0.0	0.0	0.0	2.3973686481717666E-4	0.0
7	0.0	0.0	0.0	2.3973686481717666E-4	0.0
8	5.9934216204294166E-5	0.0	0.0	2.3973686481717666E-4	0.0
9	5.9934216204294166E-5	0.0	0.0	2.9967108102147083E-4	0.0
10	5.9934216204294166E-5	0.0	0.0	4.195395134300592E-4	0.0
11	5.9934216204294166E-5	0.0	0.0	4.195395134300592E-4	0.0
12	5.9934216204294166E-5	0.0	0.0	4.794737296343533E-4	0.0
13	5.9934216204294166E-5	0.0	0.0	4.794737296343533E-4	0.0
14	5.9934216204294166E-5	0.0	0.0	4.794737296343533E-4	0.0
15	5.9934216204294166E-5	0.0	0.0	5.394079458386475E-4	5.9934216204294166E-5
16	5.9934216204294166E-5	0.0	0.0	5.993421620429417E-4	5.9934216204294166E-5
17	5.9934216204294166E-5	0.0	0.0	7.791448106558242E-4	5.9934216204294166E-5
18	5.9934216204294166E-5	0.0	0.0	0.0010188816754730008	1.1986843240858833E-4
19	5.9934216204294166E-5	0.0	0.0	0.001078815891677295	1.1986843240858833E-4
20	5.9934216204294166E-5	0.0	0.0	0.0011986843240858833	1.1986843240858833E-4
21	5.9934216204294166E-5	0.0	0.0	0.0019178949185374133	1.1986843240858833E-4
22	5.9934216204294166E-5	0.0	0.0	0.0026371055129889433	1.1986843240858833E-4
23	5.9934216204294166E-5	0.0	0.0	0.0037758556208705323	2.3973686481717666E-4
24	5.9934216204294166E-5	0.0	0.0	0.004974539944956416	2.3973686481717666E-4
25	5.9934216204294166E-5	0.0	0.0	0.00647289535006377	2.9967108102147083E-4
26	5.9934216204294166E-5	0.0	0.0	0.008690461349622654	2.9967108102147083E-4
27	5.9934216204294166E-5	0.0	0.0	0.019478620266395606	2.9967108102147083E-4
28	5.9934216204294166E-5	0.0	0.0	0.061912045339035875	2.9967108102147083E-4
29	5.9934216204294166E-5	0.0	0.0	0.12759994629894228	2.9967108102147083E-4
30	5.9934216204294166E-5	0.0	0.0	0.2115677832011584	3.59605297225765E-4
31	5.9934216204294166E-5	0.0	0.0	0.5691952512921817	3.59605297225765E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATA	35	8.87108E-4	26.428572	2
TACGCCG	45	0.003826522	20.555555	5
CTTATAC	1085	0.0	20.290323	37
CGTATAC	65	6.904517E-5	19.923077	3
ATAATAC	125	8.58563E-9	17.76	3
CGTCTAG	65	0.001580338	17.076923	1
GTATACT	110	7.812796E-7	16.818182	4
TCTTATA	1975	0.0	15.736708	37
GGACCGT	135	3.9749648E-7	15.074075	6
CCCTATA	115	2.2109167E-5	14.478261	2
GTCCTAT	185	4.9021764E-9	14.0	1
CGTTACG	310	0.0	13.725807	24
TACGGAT	755	0.0	13.721855	27
TCGTTAC	395	0.0	13.58228	23
ACGGATG	765	0.0	13.542483	28
GTATTAT	165	2.596753E-7	13.454545	1
TTATACT	220	2.8376235E-10	13.454545	4
GTATATA	195	1.0266376E-8	13.28205	1
GTCTAGT	140	9.564212E-6	13.214286	1
CTCTATA	140	9.564212E-6	13.214286	2
>>END_MODULE