FastQCFastQC Report
Thu 9 Feb 2017
SRR2727525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977721
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT25620.2620379433396644No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT17340.17735120755307496No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG16970.17356689689594476No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG16300.1667142262465468No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG14900.1523952129492974No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG9830.10053992907997271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG502.7017025E-422.24
GTACTTA2700.021.24074230
TACTTAC2550.021.03921531
CTTATAC6300.020.55555537
CATATAA1001.2891178E-820.3537
GGACCGC1101.7535058E-920.1818186
CAATACT1101.7535058E-920.1818184
CATATAC851.24492E-619.5882343
TAGGACC1052.2584572E-819.380954
AGTGTAC3500.019.0285727
TTAGGAC500.00703414918.53
GTATAGA609.235469E-418.51
ACTTACA2550.017.41176432
AGTACCG752.0668677E-417.2666665
ACATATA1107.804665E-716.81818236
TCTTATA12550.016.80478137
GCTTATA1452.9776857E-916.5862081
GTCCTAT803.3819198E-416.18751
GAATAGA803.3819198E-416.18751
GACCGCG1151.2416531E-616.0869567
Produced by FastQC (version 0.11.5)