##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727525.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977721
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44618454548895	33.0	31.0	34.0	31.0	34.0
2	32.622733888297375	34.0	31.0	34.0	31.0	34.0
3	32.71676071190043	34.0	31.0	34.0	31.0	34.0
4	36.2097224054715	37.0	35.0	37.0	35.0	37.0
5	36.05440406823624	37.0	35.0	37.0	35.0	37.0
6	36.08043092047731	37.0	35.0	37.0	35.0	37.0
7	36.051097398951235	37.0	35.0	37.0	35.0	37.0
8	36.035582748043666	37.0	35.0	37.0	35.0	37.0
9	37.73591443775883	39.0	37.0	39.0	35.0	39.0
10	37.69447009934327	39.0	37.0	39.0	35.0	39.0
11	37.7776973185602	39.0	37.0	39.0	35.0	39.0
12	37.74913395539218	39.0	37.0	39.0	35.0	39.0
13	37.78373175987833	39.0	37.0	39.0	35.0	39.0
14	39.08692766136761	40.0	38.0	41.0	36.0	41.0
15	39.0980954689528	40.0	38.0	41.0	36.0	41.0
16	39.065610741714664	40.0	38.0	41.0	36.0	41.0
17	39.08676810664801	40.0	38.0	41.0	36.0	41.0
18	39.056726816750384	40.0	38.0	41.0	36.0	41.0
19	39.10455948066984	40.0	39.0	41.0	36.0	41.0
20	39.081711449380755	40.0	39.0	41.0	36.0	41.0
21	39.05797870762723	40.0	39.0	41.0	36.0	41.0
22	39.0304350627633	40.0	39.0	41.0	36.0	41.0
23	38.981070264421035	40.0	38.0	41.0	36.0	41.0
24	38.94045131484339	40.0	38.0	41.0	35.0	41.0
25	38.880130425755404	40.0	38.0	41.0	35.0	41.0
26	38.770416100298554	40.0	38.0	41.0	35.0	41.0
27	38.714524900252734	40.0	38.0	41.0	35.0	41.0
28	38.63740269463374	40.0	38.0	41.0	35.0	41.0
29	38.539905555879436	40.0	38.0	41.0	34.0	41.0
30	38.48178161254591	40.0	38.0	41.0	34.0	41.0
31	38.409861299900484	40.0	38.0	41.0	34.0	41.0
32	38.32254702517385	40.0	38.0	41.0	34.0	41.0
33	38.278718571044294	40.0	38.0	41.0	34.0	41.0
34	38.22332137695723	40.0	38.0	41.0	34.0	41.0
35	38.219039991981354	40.0	38.0	41.0	34.0	41.0
36	38.14947208866333	40.0	38.0	41.0	34.0	41.0
37	38.14579517060593	40.0	38.0	41.0	34.0	41.0
38	38.11539999652253	40.0	38.0	41.0	33.0	41.0
39	38.067631768162904	40.0	38.0	41.0	33.0	41.0
40	38.00577772186544	40.0	38.0	41.0	33.0	41.0
41	37.95455963408784	40.0	37.0	41.0	33.0	41.0
42	37.91279209508643	40.0	37.0	41.0	33.0	41.0
43	36.768821575889234	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	0.0
13	0.0
14	0.0
15	1.0
16	5.0
17	5.0
18	11.0
19	18.0
20	57.0
21	111.0
22	236.0
23	457.0
24	743.0
25	1228.0
26	1940.0
27	3087.0
28	4429.0
29	6153.0
30	8813.0
31	12164.0
32	16386.0
33	22069.0
34	31363.0
35	45529.0
36	68438.0
37	116350.0
38	268373.0
39	369750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58073519950988	16.405088977325843	12.396481204760867	27.61769461840341
2	18.353497572415854	19.629321657200776	35.297083728384685	26.720097041998685
3	20.595650497432295	21.350671612862975	29.13131660258908	28.92236128711565
4	16.052841250213508	14.207222714864464	32.097091092448665	37.64284494247336
5	17.03880759439554	34.45471663184078	32.395437962363495	16.111037811400184
6	38.11762251194359	31.654326745564433	14.120388127083288	16.107662615408692
7	30.831085759639	29.593207060091785	19.852084592639414	19.723622587629805
8	28.23085522352491	34.40460008530041	18.95745309756055	18.40709159361413
9	29.021367036199486	14.142275761694798	16.962814545253707	39.87354265685201
10	17.42204575742978	25.807975894963903	31.83863290243331	24.931345445173008
11	38.01104814154549	20.625822704022927	19.25201565681825	22.111113497613328
12	23.012597663341587	23.042565312599404	28.486449610880815	25.458387413178198
13	33.19515485501488	18.149144796930823	22.50345446195796	26.152245886096342
14	24.041930162081	18.953361950904192	23.913468157071392	33.09123972994341
15	28.397876285770685	25.130482008671184	20.860552243431407	25.611089462126724
16	26.192748237994273	24.16599418443503	23.600290880527268	26.040966697043434
17	26.033909469061218	24.241373561578406	23.683647993650542	26.04106897570984
18	25.94298373462368	24.18767726171372	24.658261405861182	25.211077597801417
19	27.254093959319682	24.469352709003896	23.742253669502855	24.534299662173563
20	25.971008089219726	23.2946822252974	24.001836924848703	26.732472760634167
21	25.462274002501733	25.04528387955255	24.34845932530855	25.143982792637164
22	26.714369436679792	23.59977948719522	22.69195404414961	26.99389703197538
23	25.96937163055718	23.551810792649437	23.74839038948739	26.730427187305988
24	25.299446365578724	23.473158498180975	24.063817796692515	27.163577339547786
25	26.48056040526899	22.821438835823308	23.183198478911674	27.514802279996033
26	25.936949293305556	23.19567647621356	24.411667541149264	26.45570668933162
27	27.106301286358786	23.80556416400998	23.590983521884056	25.49715102774718
28	26.213817643274513	24.739879781655503	22.550809484505294	26.495493090564693
29	25.758677577754803	24.341095261327105	24.434475683758457	25.465751477159635
30	27.05823031314659	23.508137802092826	23.53800317268423	25.895628712076352
31	26.05242190768123	23.39757456370478	24.127537405865272	26.422466122748716
32	25.49060519309701	23.664726440364888	23.840134353256197	27.004534013281905
33	26.027670470410268	23.244770236089845	23.66493099769771	27.062628295802178
34	26.436171464047515	23.720468313557753	24.575211128737134	25.2681490936576
35	26.091799194248665	23.402279382359588	24.324832953368087	26.181088470023656
36	25.500117109073038	23.147605503001365	24.814134093468383	26.53814329445721
37	24.382211285223494	22.745854901347112	25.790588521674383	27.08134529175501
38	25.713572685868463	21.96025246466016	25.280524812293077	27.04565003717829
39	24.22286112295839	22.31720501042731	26.655968318160294	26.803965548454006
40	25.79406599633229	21.28153123437054	27.11929067699272	25.805112092304448
41	24.706434657739784	21.853678094262065	26.901846232207347	26.538041015790803
42	23.773755498756802	21.776559979789738	28.03601436401591	26.41367015743755
43	23.35584486780994	21.348626039534796	27.46550396278693	27.830025129868336
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	16.0
2	24.0
3	60.5
4	97.0
5	97.0
6	114.5
7	132.0
8	141.0
9	150.0
10	192.5
11	235.0
12	235.0
13	458.0
14	681.0
15	1081.0
16	1481.0
17	1385.0
18	1289.0
19	1289.0
20	1528.5
21	1768.0
22	1805.5
23	1843.0
24	2385.0
25	2927.0
26	2927.0
27	3701.5
28	4476.0
29	5598.0
30	6720.0
31	8717.5
32	10715.0
33	10715.0
34	13475.0
35	16235.0
36	19191.0
37	22147.0
38	26502.5
39	30858.0
40	30858.0
41	35756.0
42	40654.0
43	46774.0
44	52894.0
45	61474.5
46	70055.0
47	70055.0
48	80792.5
49	91530.0
50	100102.5
51	108675.0
52	113910.5
53	119146.0
54	119146.0
55	108779.0
56	98412.0
57	88465.0
58	78518.0
59	73738.5
60	68959.0
61	68959.0
62	60368.0
63	51777.0
64	43356.0
65	34935.0
66	29126.0
67	23317.0
68	23317.0
69	19053.5
70	14790.0
71	12007.5
72	9225.0
73	7427.5
74	5630.0
75	5630.0
76	4562.0
77	3494.0
78	2761.0
79	2028.0
80	1532.0
81	1036.0
82	1036.0
83	778.5
84	521.0
85	372.5
86	224.0
87	150.5
88	77.0
89	77.0
90	49.5
91	22.0
92	16.5
93	11.0
94	8.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	977721.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.27816701578166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.45793485763672	58.34911149033509
2	8.148534169461469	11.127339539125877
3	2.610824247241294	5.347868820059806
4	1.2381088989168023	3.3814322473586786
5	0.7099606204163045	2.4237404907706206
6	0.44479312139960164	1.8221795418235713
7	0.294638644696143	1.4082170614306762
8	0.20990361272637043	1.1465467141557657
9	0.16236262702522963	0.9977240308634724
>10	0.6683138634045879	7.574885641349968
>50	0.028968912201186258	1.3766664299381426
>100	0.022955749095303325	3.1760371102914657
>500	0.0019504880625663178	0.9346888771125375
>1k	7.501877163716607E-4	0.9335620053843221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2562	0.2620379433396644	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	1734	0.17735120755307496	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	1697	0.17356689689594476	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1630	0.1667142262465468	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	1490	0.1523952129492974	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	983	0.10053992907997271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0455733281784884E-4
2	0.0	0.0	0.0	0.0	2.0455733281784884E-4
3	0.0	0.0	0.0	0.0	2.0455733281784884E-4
4	0.0	0.0	0.0	1.0227866640892442E-4	2.0455733281784884E-4
5	0.0	0.0	0.0	1.0227866640892442E-4	2.0455733281784884E-4
6	0.0	0.0	0.0	1.0227866640892442E-4	2.0455733281784884E-4
7	0.0	0.0	0.0	1.0227866640892442E-4	2.0455733281784884E-4
8	0.0	0.0	0.0	1.0227866640892442E-4	2.0455733281784884E-4
9	0.0	0.0	0.0	3.068359992267733E-4	2.0455733281784884E-4
10	0.0	0.0	0.0	3.068359992267733E-4	2.0455733281784884E-4
11	0.0	0.0	0.0	3.068359992267733E-4	2.0455733281784884E-4
12	0.0	0.0	0.0	3.068359992267733E-4	2.0455733281784884E-4
13	0.0	0.0	0.0	3.068359992267733E-4	2.0455733281784884E-4
14	0.0	0.0	0.0	3.068359992267733E-4	2.0455733281784884E-4
15	0.0	0.0	0.0	5.113933320446221E-4	3.068359992267733E-4
16	0.0	0.0	0.0	7.15950664862471E-4	3.068359992267733E-4
17	0.0	0.0	0.0	7.15950664862471E-4	3.068359992267733E-4
18	0.0	0.0	0.0	8.182293312713954E-4	4.091146656356977E-4
19	0.0	0.0	0.0	9.205079976803199E-4	4.091146656356977E-4
20	0.0	0.0	0.0	0.0012273439969070932	4.091146656356977E-4
21	0.0	0.0	0.0	0.0018410159953606397	4.091146656356977E-4
22	0.0	0.0	0.0	0.002863802659449884	4.091146656356977E-4
23	0.0	0.0	0.0	0.004602539988401599	6.136719984535466E-4
24	0.0	0.0	0.0	0.007670899980669332	6.136719984535466E-4
25	1.0227866640892442E-4	0.0	0.0	0.009409637309621048	7.15950664862471E-4
26	1.0227866640892442E-4	0.0	0.0	0.013807619965204798	7.15950664862471E-4
27	1.0227866640892442E-4	0.0	0.0	0.025978781267866805	7.15950664862471E-4
28	1.0227866640892442E-4	0.0	0.0	0.07384519714724344	7.15950664862471E-4
29	1.0227866640892442E-4	0.0	0.0	0.14595165696553516	7.15950664862471E-4
30	1.0227866640892442E-4	0.0	0.0	0.2466961433783257	7.15950664862471E-4
31	1.0227866640892442E-4	0.0	0.0	0.6545834650171163	7.15950664862471E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACACG	50	2.7017025E-4	22.2	4
GTACTTA	270	0.0	21.240742	30
TACTTAC	255	0.0	21.039215	31
CTTATAC	630	0.0	20.555555	37
CATATAA	100	1.2891178E-8	20.35	37
GGACCGC	110	1.7535058E-9	20.181818	6
CAATACT	110	1.7535058E-9	20.181818	4
CATATAC	85	1.24492E-6	19.588234	3
TAGGACC	105	2.2584572E-8	19.38095	4
AGTGTAC	350	0.0	19.02857	27
TTAGGAC	50	0.007034149	18.5	3
GTATAGA	60	9.235469E-4	18.5	1
ACTTACA	255	0.0	17.411764	32
AGTACCG	75	2.0668677E-4	17.266666	5
ACATATA	110	7.804665E-7	16.818182	36
TCTTATA	1255	0.0	16.804781	37
GCTTATA	145	2.9776857E-9	16.586208	1
GTCCTAT	80	3.3819198E-4	16.1875	1
GAATAGA	80	3.3819198E-4	16.1875	1
GACCGCG	115	1.2416531E-6	16.086956	7
>>END_MODULE