FastQCFastQC Report
Thu 9 Feb 2017
SRR2727524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415388
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12600.020.70238137
TCTTATA24200.017.50619937
CGACTTA1201.9375511E-615.41666719
GCTTATA1752.242814E-914.7999991
GTATAAT1803.3360266E-914.3888881
CTCGTTA4900.013.96938722
GGTATCA13000.013.9461541
GGATACG1356.5736695E-613.7037041
GTCTTAG3750.013.321
TCGTTAC4650.013.12903223
CGTTACG3700.013.024
CGGTAAA1000.001833495912.95000134
CTAGCGT1000.001833495912.9500014
TAGACTG3650.012.6712335
TTATAAC2052.0683729E-812.6341473
CTCTTAT37050.012.43319837
TTTAGAC2756.184564E-1112.1090923
GTATATG2002.042434E-712.0250011
GTCCTAT1851.0405547E-612.01
TATATGA1552.7225933E-511.9354832
Produced by FastQC (version 0.11.5)