##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727524.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2415388
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61296280349161	34.0	31.0	34.0	31.0	34.0
2	32.782912724580896	34.0	31.0	34.0	31.0	34.0
3	32.878984660021494	34.0	31.0	34.0	31.0	34.0
4	36.32985880529339	37.0	37.0	37.0	35.0	37.0
5	36.19388189392346	37.0	35.0	37.0	35.0	37.0
6	36.209226012549536	37.0	36.0	37.0	35.0	37.0
7	36.18825422665013	37.0	36.0	37.0	35.0	37.0
8	36.173483100851705	37.0	36.0	37.0	35.0	37.0
9	37.89732415661583	39.0	38.0	39.0	35.0	39.0
10	37.874238010621895	39.0	38.0	39.0	35.0	39.0
11	37.950214209890916	39.0	38.0	39.0	35.0	39.0
12	37.91771591148089	39.0	38.0	39.0	35.0	39.0
13	37.94869561329277	39.0	38.0	39.0	35.0	39.0
14	39.31242392526584	40.0	39.0	41.0	36.0	41.0
15	39.33085367651077	40.0	39.0	41.0	36.0	41.0
16	39.31930936147733	40.0	39.0	41.0	36.0	41.0
17	39.328621322951015	40.0	39.0	41.0	36.0	41.0
18	39.30064196725329	40.0	39.0	41.0	36.0	41.0
19	39.334167843841236	40.0	39.0	41.0	36.0	41.0
20	39.30683931525701	40.0	39.0	41.0	36.0	41.0
21	39.287027177414146	40.0	39.0	41.0	36.0	41.0
22	39.25384368888145	40.0	39.0	41.0	36.0	41.0
23	39.214181324077124	40.0	39.0	41.0	36.0	41.0
24	39.1705663851936	40.0	39.0	41.0	36.0	41.0
25	39.109599368714264	40.0	39.0	41.0	36.0	41.0
26	39.015815678474844	40.0	39.0	41.0	36.0	41.0
27	38.97228022992579	40.0	39.0	41.0	36.0	41.0
28	38.91362629937716	40.0	38.0	41.0	35.0	41.0
29	38.83919395144797	40.0	38.0	41.0	35.0	41.0
30	38.778265024087226	40.0	38.0	41.0	35.0	41.0
31	38.71535215046195	40.0	38.0	41.0	35.0	41.0
32	38.633860067202455	40.0	38.0	41.0	35.0	41.0
33	38.58714127916509	40.0	38.0	41.0	35.0	41.0
34	38.54448933256272	40.0	38.0	41.0	35.0	41.0
35	38.53179282169159	40.0	38.0	41.0	35.0	41.0
36	38.482817667389256	40.0	38.0	41.0	34.0	41.0
37	38.461962632918606	40.0	38.0	41.0	34.0	41.0
38	38.413457382416404	40.0	38.0	41.0	34.0	41.0
39	38.374576258555564	40.0	38.0	41.0	34.0	41.0
40	38.31661786843356	40.0	38.0	41.0	34.0	41.0
41	38.276347319768085	40.0	38.0	41.0	34.0	41.0
42	38.23448655040101	40.0	38.0	41.0	34.0	41.0
43	37.12784281448778	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	2.0
12	0.0
13	0.0
14	0.0
15	2.0
16	9.0
17	7.0
18	31.0
19	54.0
20	113.0
21	227.0
22	508.0
23	929.0
24	1490.0
25	2372.0
26	3748.0
27	5804.0
28	8416.0
29	12196.0
30	17404.0
31	24273.0
32	33365.0
33	45876.0
34	66342.0
35	97652.0
36	150866.0
37	260731.0
38	610495.0
39	1072470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.684120315245416	16.640018084051093	11.670547340634299	28.00531426006919
2	19.011562531568426	19.188511328200686	34.4728465985589	27.327079541671978
3	20.402187971456346	20.92144202090927	28.59370834002653	30.08266166760785
4	15.609748827103553	14.285986350847152	32.55137476877421	37.552890053275085
5	16.602963995846633	34.34417161963212	33.069055572024034	15.983808812497205
6	37.91175579244411	32.18857591409744	14.472250421050365	15.427417872408078
7	31.273029426328193	28.58083256189068	20.139414454323695	20.006723557457434
8	27.964616864868084	33.497226946560964	18.924454373376037	19.613701815194908
9	28.087412871141197	13.777248210225437	17.549188784576224	40.586150134057135
10	17.249485382886725	25.500582101095144	32.45325388716016	24.796678628857972
11	38.247271245861945	20.10960557889664	20.21811816569429	21.42500500954712
12	22.90360803316072	22.99634675671155	28.924918066993794	25.175127143133942
13	32.321846427985896	18.50944858548606	22.652965072278242	26.515739914249803
14	23.79601124125813	18.74071577734095	23.572320471907616	33.8909525094933
15	26.69575240085651	25.902960518144496	20.80961733684195	26.59166974415705
16	26.32500451273253	24.46919501131909	22.793025385569525	26.412775090378855
17	25.79742881888955	24.34627480139837	23.745460356679754	26.110836023032324
18	25.952807582053072	24.167338746404305	24.125068105000107	25.75478556654252
19	26.943207468116924	23.850122630401412	23.893179894907153	25.31349000657451
20	26.339412135855607	23.611858633064337	23.88605888577736	26.1626703453027
21	26.01942213838936	23.981364484712188	24.183029807219377	25.816183569679076
22	26.710408431274807	23.447537207272703	23.431266529435437	26.410787832017053
23	26.15335507173175	23.65309424407176	23.773902991983068	26.419647692213427
24	25.959473177808285	23.653218447719375	23.807189569543276	26.580118804929064
25	26.464361005354004	23.280234893938363	23.684931779076486	26.57047232163114
26	26.055151387685953	23.662575122506198	23.979874040940835	26.302399448867014
27	26.447096698335837	23.954370891964356	23.457183690570623	26.14134871912918
28	26.16089009302025	24.15450436948432	23.255642571711046	26.42896296578438
29	26.09382012330938	24.195698579275877	23.840062134944777	25.870419162469965
30	26.55850737024445	23.808680013314632	23.476352453518857	26.156460162922063
31	26.19123718425363	23.61566754492446	23.901046125922626	26.292049144899288
32	25.716448040646057	23.914170311353704	23.876371001263568	26.493010646736675
33	25.776852414601713	23.608836344305757	23.91619897093138	26.69811227016115
34	26.252221175231472	23.50479508882217	24.374386226974714	25.868597508971643
35	26.086864719043067	23.40269969048451	24.179717709949706	26.330717880522712
36	25.741330171384476	23.160585380071442	24.58942414220821	26.508660306335873
37	25.403454848661994	22.761187850564795	25.05001266877206	26.785344632001152
38	25.464066228697003	22.596700819909678	25.15902207016016	26.780210881233156
39	24.909828151833164	22.633092488660207	26.018304305560847	26.438775053945783
40	25.465059857877907	22.07579072182192	26.190326357504468	26.26882306279571
41	24.732672349121547	22.299605694820045	26.403666822887256	26.56405513317115
42	24.208905567138693	22.477009904826886	26.697201443411988	26.61688308462243
43	23.881794560542655	22.062252524232132	26.692812914529675	27.36314000069554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	46.0
2	59.0
3	119.5
4	180.0
5	180.0
6	202.5
7	225.0
8	223.5
9	222.0
10	317.0
11	412.0
12	412.0
13	686.5
14	961.0
15	1342.5
16	1724.0
17	1803.0
18	1882.0
19	1882.0
20	2478.5
21	3075.0
22	3828.5
23	4582.0
24	6234.5
25	7887.0
26	7887.0
27	10439.0
28	12991.0
29	16245.5
30	19500.0
31	24507.0
32	29514.0
33	29514.0
34	36880.5
35	44247.0
36	53317.0
37	62387.0
38	74349.5
39	86312.0
40	86312.0
41	99413.5
42	112515.0
43	128085.5
44	143656.0
45	161300.0
46	178944.0
47	178944.0
48	196006.5
49	213069.0
50	225672.0
51	238275.0
52	239984.5
53	241694.0
54	241694.0
55	234658.0
56	227622.0
57	215499.0
58	203376.0
59	189354.5
60	175333.0
61	175333.0
62	155462.5
63	135592.0
64	115672.0
65	95752.0
66	80546.5
67	65341.0
68	65341.0
69	53728.5
70	42116.0
71	34503.0
72	26890.0
73	21763.5
74	16637.0
75	16637.0
76	13445.0
77	10253.0
78	8198.5
79	6144.0
80	4769.0
81	3394.0
82	3394.0
83	2432.0
84	1470.0
85	1097.0
86	724.0
87	500.0
88	276.0
89	276.0
90	177.5
91	79.0
92	53.5
93	28.0
94	19.0
95	10.0
96	10.0
97	7.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2415388.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.84140926039203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4009606445396	53.42994412602049
2	9.460329096510458	12.26842141369659
3	3.1261751140927796	6.081167999776279
4	1.4809752522412427	3.8411408974034695
5	0.906081927172605	2.937581453162181
6	0.556136810118068	2.163641670578075
7	0.4088663076493989	1.8558027310956022
8	0.28535805659823654	1.4802414826909094
9	0.20762521690036922	1.2116440495633056
>10	1.1261450569249143	12.389790005276396
>50	0.03346392451555221	1.4589860650867432
>100	0.007627101894342283	0.7820741241337753
>500	2.554908424188908E-4	0.09956398151613627
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.1401215870907696E-5
2	0.0	0.0	0.0	0.0	4.1401215870907696E-5
3	0.0	0.0	0.0	0.0	4.1401215870907696E-5
4	0.0	0.0	0.0	0.0	4.1401215870907696E-5
5	0.0	0.0	0.0	0.0	4.1401215870907696E-5
6	0.0	0.0	0.0	0.0	8.280243174181539E-5
7	4.1401215870907696E-5	0.0	0.0	0.0	8.280243174181539E-5
8	4.1401215870907696E-5	0.0	0.0	0.0	8.280243174181539E-5
9	4.1401215870907696E-5	0.0	0.0	0.0	2.070060793545385E-4
10	4.1401215870907696E-5	0.0	0.0	0.0	2.070060793545385E-4
11	4.1401215870907696E-5	0.0	0.0	0.0	2.070060793545385E-4
12	4.1401215870907696E-5	0.0	0.0	0.0	2.070060793545385E-4
13	4.1401215870907696E-5	0.0	0.0	0.0	2.070060793545385E-4
14	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.0	2.070060793545385E-4
15	4.1401215870907696E-5	4.1401215870907696E-5	0.0	8.280243174181539E-5	3.3120972696726157E-4
16	4.1401215870907696E-5	4.1401215870907696E-5	0.0	8.280243174181539E-5	4.5541337457998465E-4
17	4.1401215870907696E-5	4.1401215870907696E-5	0.0	8.280243174181539E-5	4.5541337457998465E-4
18	4.1401215870907696E-5	4.1401215870907696E-5	0.0	2.070060793545385E-4	4.5541337457998465E-4
19	4.1401215870907696E-5	4.1401215870907696E-5	0.0	2.898085110963539E-4	4.5541337457998465E-4
20	4.1401215870907696E-5	4.1401215870907696E-5	0.0	2.898085110963539E-4	4.5541337457998465E-4
21	4.1401215870907696E-5	4.1401215870907696E-5	0.0	4.968145904508923E-4	5.382158063218001E-4
22	4.1401215870907696E-5	4.1401215870907696E-5	0.0	7.452218856763386E-4	5.382158063218001E-4
23	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.0010350303967726923	6.624194539345231E-4
24	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.001366240123739954	7.038206698054308E-4
25	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.002194264441158108	7.038206698054308E-4
26	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.003974516723607139	7.038206698054308E-4
27	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.014117814611979525	7.038206698054308E-4
28	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.048273817705478374	7.038206698054308E-4
29	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.0984106901251476	7.038206698054308E-4
30	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.16552206105188896	7.038206698054308E-4
31	4.1401215870907696E-5	4.1401215870907696E-5	0.0	0.4388114870157507	7.038206698054308E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1260	0.0	20.702381	37
TCTTATA	2420	0.0	17.506199	37
CGACTTA	120	1.9375511E-6	15.416667	19
GCTTATA	175	2.242814E-9	14.799999	1
GTATAAT	180	3.3360266E-9	14.388888	1
CTCGTTA	490	0.0	13.969387	22
GGTATCA	1300	0.0	13.946154	1
GGATACG	135	6.5736695E-6	13.703704	1
GTCTTAG	375	0.0	13.32	1
TCGTTAC	465	0.0	13.129032	23
CGTTACG	370	0.0	13.0	24
CGGTAAA	100	0.0018334959	12.950001	34
CTAGCGT	100	0.0018334959	12.950001	4
TAGACTG	365	0.0	12.671233	5
TTATAAC	205	2.0683729E-8	12.634147	3
CTCTTAT	3705	0.0	12.433198	37
TTTAGAC	275	6.184564E-11	12.109092	3
GTATATG	200	2.042434E-7	12.025001	1
GTCCTAT	185	1.0405547E-6	12.0	1
TATATGA	155	2.7225933E-5	11.935483	2
>>END_MODULE