FastQCFastQC Report
Thu 9 Feb 2017
SRR2727523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022541
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10500.020.08571437
CAATACG656.905445E-519.9230774
ATACCGT851.246266E-619.5882366
ATACTAG752.068245E-417.2666661
TACCGTA650.00158049517.0769237
TCTTATA18950.016.69393237
TACTTAC3050.016.3770531
CTCGTTA4300.015.48837222
GATATCG855.366654E-415.2352945
GTATCGC750.0041058414.86
AATACCG750.0041058414.85
TCGTTAC3750.014.79999923
GTATATG1803.3342076E-914.3888891
CCTAATA1551.211447E-714.3225812
GTACCGT1702.4394467E-814.1470596
CTCTATA1702.4394467E-814.1470592
TAATACT2500.014.0599994
TTAATAC1601.7852653E-713.8750013
AATACGT800.006300801413.8750015
GGGCGTC5250.013.74285811
Produced by FastQC (version 0.11.5)