FastQCFastQC Report
Thu 9 Feb 2017
SRR2727522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107699
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10400.021.34615537
TACTTAC6500.020.77692231
GGTATCA9550.019.3717271
GTACTTA7250.018.8827630
TCTTATA20250.017.81481437
TATACCG953.6092733E-617.5263165
TTTCGTA650.001580524517.07692516
TTACATA4350.017.01149634
ATATACT1801.0913936E-1116.4444454
GTATTAG1708.54925E-1116.323531
ACTTACA6550.015.81679332
TATATAC1902.7284841E-1115.5789483
CATATAA3150.015.26984237
TACATAT4000.015.26249935
ACGACCG750.00410591614.85
GTATAGA1651.6470949E-814.5757571
GTCTTAG3050.014.5573771
AGTGTAC9950.014.50251327
CTTACAT5800.014.35344833
TATTAGG1551.2115197E-714.3225812
Produced by FastQC (version 0.11.5)