##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727522.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2107699
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.077360666774524	33.0	31.0	34.0	30.0	34.0
2	32.27383464147395	34.0	31.0	34.0	30.0	34.0
3	32.36727065866616	34.0	31.0	34.0	30.0	34.0
4	35.976861496826636	37.0	35.0	37.0	35.0	37.0
5	35.698401906534095	37.0	35.0	37.0	33.0	37.0
6	35.697659865094586	37.0	35.0	37.0	33.0	37.0
7	35.67343202231438	37.0	35.0	37.0	33.0	37.0
8	35.644614340093156	37.0	35.0	37.0	33.0	37.0
9	37.31060981667686	39.0	37.0	39.0	34.0	39.0
10	37.20649485529006	39.0	37.0	39.0	34.0	39.0
11	37.30866219512369	39.0	37.0	39.0	34.0	39.0
12	37.26165073855422	39.0	37.0	39.0	34.0	39.0
13	37.31611060213057	39.0	37.0	39.0	34.0	39.0
14	38.53809059073426	40.0	38.0	41.0	35.0	41.0
15	38.52850525620594	40.0	38.0	41.0	34.0	41.0
16	38.51009987669017	40.0	38.0	41.0	34.0	41.0
17	38.518166018961914	40.0	38.0	41.0	34.0	41.0
18	38.48062365641394	40.0	38.0	41.0	34.0	41.0
19	38.5202749538715	40.0	38.0	41.0	34.0	41.0
20	38.496774444548294	40.0	38.0	41.0	34.0	41.0
21	38.469463144405346	40.0	38.0	41.0	34.0	41.0
22	38.44337782577114	40.0	38.0	41.0	34.0	41.0
23	38.38997171797301	40.0	38.0	41.0	34.0	41.0
24	38.33334978096968	40.0	38.0	41.0	34.0	41.0
25	38.25901041847057	40.0	38.0	41.0	34.0	41.0
26	38.13166680821123	40.0	38.0	41.0	34.0	41.0
27	38.075024944263866	40.0	38.0	41.0	34.0	41.0
28	37.99262703070979	40.0	38.0	41.0	33.0	41.0
29	37.8963931756859	40.0	37.0	41.0	33.0	41.0
30	37.835422420374066	40.0	37.0	41.0	33.0	41.0
31	37.756878472685145	40.0	37.0	41.0	33.0	41.0
32	37.657421671690315	40.0	37.0	41.0	33.0	41.0
33	37.60649172391314	39.0	37.0	41.0	33.0	41.0
34	37.5532289003316	39.0	37.0	41.0	32.0	41.0
35	37.54595746356572	39.0	37.0	41.0	32.0	41.0
36	37.48360985131178	39.0	37.0	40.0	32.0	41.0
37	37.459163761049375	39.0	37.0	40.0	32.0	41.0
38	37.42649922972873	39.0	37.0	40.0	32.0	41.0
39	37.368704924185096	39.0	37.0	40.0	32.0	41.0
40	37.313601230536236	39.0	36.0	40.0	32.0	41.0
41	37.268375607712485	39.0	36.0	40.0	32.0	41.0
42	37.23522096845897	39.0	36.0	40.0	32.0	41.0
43	36.10566072290209	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	1.0
14	3.0
15	7.0
16	11.0
17	23.0
18	57.0
19	105.0
20	245.0
21	475.0
22	902.0
23	1600.0
24	2690.0
25	4243.0
26	6555.0
27	9573.0
28	14048.0
29	19598.0
30	26943.0
31	36033.0
32	48442.0
33	64655.0
34	89518.0
35	128048.0
36	195221.0
37	335172.0
38	646976.0
39	476550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69044109239507	17.16426301858093	12.362865855133965	27.782430033890037
2	18.72231281601405	19.43930324016854	35.31557399799497	26.522809945822434
3	20.099833989578208	21.38597589124443	29.142870969716263	29.371319149461094
4	15.796657871925735	14.634252803649858	32.892125488506665	36.676963835917746
5	16.28923295024574	35.135994276222554	33.04826732849425	15.526505445037456
6	37.490979499444656	32.82537022601424	14.60213246768158	15.081517806859518
7	31.155349981188017	29.56162146492455	20.01490725193683	19.26812130195061
8	27.86137868832314	34.90128334264048	18.745987923323018	18.491350045713357
9	28.731521910861087	13.948054252528468	17.25896344781679	40.061460388793655
10	16.853070576016783	26.187515390005878	32.39959785529148	24.559816178685857
11	38.315243305614324	20.411738108714765	20.158049133201658	21.114969452469257
12	22.694369547074796	23.134944790503766	29.161706676332816	25.00897898608862
13	32.11905495044596	18.710593875121635	22.932543973309283	26.23780720112312
14	23.515644311640322	18.728290899222326	23.863227149607226	33.89283763953012
15	26.7688602594583	26.116300287659673	20.919970071627876	26.194869381254154
16	25.837038400644495	25.009643217556203	23.131860858689972	26.021457523109326
17	25.67491847744863	24.476123013769993	23.63012934958929	26.218829159192087
18	25.67942576240725	24.938475560314828	24.158905042892748	25.223193634385176
19	27.197669116890033	24.150554704443092	23.871530042952052	24.780246135714826
20	25.998446647267944	23.7949061986555	23.975909273572746	26.230737880503813
21	25.76458972557277	24.383699949565855	24.69655297079896	25.155157354062418
22	26.594499499216916	23.43607887084446	23.469053218699635	26.500368411238984
23	25.836042053443116	23.909770797443088	24.329754865376888	25.92443228373691
24	25.45254327112173	23.689957626776877	24.30906879967206	26.54843030242933
25	26.2181649277245	23.425213941838944	23.80942440073274	26.547196729703813
26	25.74774671335898	23.732041434758948	24.370937216367235	26.149274635514843
27	26.51147056576864	24.205685916252747	23.619216975478945	25.663626542499664
28	25.877651410376906	24.799651183589308	22.976478140379626	26.34621926565416
29	25.85454564432587	24.73337037214517	24.01984344064309	25.39224054288587
30	27.012822988481748	23.873665072669294	23.2676013036017	25.845910635247254
31	26.136749127840357	23.886570141182396	24.085887026563093	25.89079370441415
32	25.63112664569277	24.22490118370792	23.979989552587917	26.163982618011396
33	25.60104644923208	23.60716591885274	24.090678982150678	26.701108649764503
34	26.112409789063808	23.80747915143481	24.79590302030793	25.28420803919345
35	26.095614221954843	23.53281943958791	24.213609248758956	26.157957089698293
36	25.632217883103802	23.35527985732308	24.84249411324862	26.1700081463245
37	25.13874134779207	23.17693370827618	25.411550700550695	26.272774243381054
38	25.711403763061046	22.65100472126238	25.06501165489	26.572579860786572
39	24.6665202194431	23.153638161805837	26.16137313724588	26.018468481505185
40	25.830158860444495	22.25716290608858	26.076114283870705	25.83656394959622
41	24.672640637965856	22.810183047958933	26.273058914010015	26.244117400065193
42	24.4111232201562	22.86574126571204	26.54809818669554	26.175037327436222
43	23.824891504906535	22.490924937574103	26.329091582811397	27.355091974707964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	46.0
2	56.0
3	91.5
4	127.0
5	127.0
6	181.5
7	236.0
8	222.5
9	209.0
10	295.5
11	382.0
12	382.0
13	615.5
14	849.0
15	1243.0
16	1637.0
17	1749.5
18	1862.0
19	1862.0
20	2434.5
21	3007.0
22	3691.0
23	4375.0
24	6025.5
25	7676.0
26	7676.0
27	9969.0
28	12262.0
29	15138.5
30	18015.0
31	22898.0
32	27781.0
33	27781.0
34	33890.0
35	39999.0
36	48304.0
37	56609.0
38	66802.5
39	76996.0
40	76996.0
41	88679.5
42	100363.0
43	113926.0
44	127489.0
45	144649.5
46	161810.0
47	161810.0
48	178351.0
49	194892.0
50	207258.0
51	219624.0
52	218875.5
53	218127.0
54	218127.0
55	207937.5
56	197748.0
57	184741.5
58	171735.0
59	159873.0
60	148011.0
61	148011.0
62	129844.0
63	111677.0
64	93536.0
65	75395.0
66	62999.5
67	50604.0
68	50604.0
69	41440.5
70	32277.0
71	25875.0
72	19473.0
73	15576.0
74	11679.0
75	11679.0
76	9379.5
77	7080.0
78	5506.5
79	3933.0
80	3008.0
81	2083.0
82	2083.0
83	1509.5
84	936.0
85	688.0
86	440.0
87	295.0
88	150.0
89	150.0
90	97.5
91	45.0
92	25.0
93	5.0
94	6.5
95	8.0
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2107699.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.62416460397183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.88826619872712	53.56584958564377
2	9.161629226186562	11.84125270307279
3	3.066761643537296	5.945607277593042
4	1.4691948676043733	3.7978196383750245
5	0.8155285843669434	2.6351426737686743
6	0.5609995930090573	2.175247802482712
7	0.40080492100544396	1.8131178232396419
8	0.2779698496696076	1.4370855455992022
9	0.21581812883287058	1.2552359653994964
>10	1.090642118829447	11.932331507296107
>50	0.037727409543794596	1.640620631151037
>100	0.013774424117353318	1.575022044557423
>500	7.358621418410268E-4	0.2854695205095316
>1k	1.4717242836818903E-4	0.10019728131159819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.744510482758686E-5
2	0.0	0.0	0.0	0.0	4.744510482758686E-5
3	0.0	0.0	0.0	0.0	4.744510482758686E-5
4	0.0	0.0	0.0	0.0	1.4233531448276058E-4
5	0.0	0.0	0.0	0.0	1.4233531448276058E-4
6	0.0	0.0	0.0	0.0	1.4233531448276058E-4
7	0.0	0.0	0.0	0.0	1.4233531448276058E-4
8	0.0	0.0	0.0	0.0	1.4233531448276058E-4
9	0.0	0.0	0.0	0.0	2.3722552413793431E-4
10	0.0	0.0	0.0	0.0	2.3722552413793431E-4
11	0.0	0.0	0.0	4.744510482758686E-5	2.3722552413793431E-4
12	0.0	0.0	0.0	4.744510482758686E-5	2.3722552413793431E-4
13	0.0	0.0	0.0	4.744510482758686E-5	2.3722552413793431E-4
14	0.0	0.0	0.0	4.744510482758686E-5	2.8467062896552116E-4
15	0.0	4.744510482758686E-5	0.0	4.744510482758686E-5	3.3211573379310805E-4
16	0.0	4.744510482758686E-5	0.0	9.489020965517372E-5	3.795608386206949E-4
17	0.0	4.744510482758686E-5	0.0	1.8978041931034745E-4	3.795608386206949E-4
18	0.0	4.744510482758686E-5	0.0	3.3211573379310805E-4	4.7445104827586863E-4
19	0.0	4.744510482758686E-5	0.0	4.7445104827586863E-4	4.7445104827586863E-4
20	0.0	4.744510482758686E-5	0.0	5.218961531034555E-4	4.7445104827586863E-4
21	0.0	4.744510482758686E-5	0.0	6.167863627586292E-4	4.7445104827586863E-4
22	0.0	4.744510482758686E-5	0.0	9.014569917241504E-4	4.7445104827586863E-4
23	0.0	4.744510482758686E-5	0.0	0.0011386825158620846	6.167863627586292E-4
24	0.0	4.744510482758686E-5	0.0	0.0014233531448276059	6.167863627586292E-4
25	0.0	4.744510482758686E-5	0.0	0.0018503590882758877	6.167863627586292E-4
26	0.0	4.744510482758686E-5	0.0	0.003083931813793146	6.167863627586292E-4
27	0.0	4.744510482758686E-5	0.0	0.010390477957241523	6.167863627586292E-4
28	0.0	4.744510482758686E-5	0.0	0.03805097407172466	6.642314675862161E-4
29	0.0	4.744510482758686E-5	0.0	0.0808939037310356	6.642314675862161E-4
30	0.0	4.744510482758686E-5	0.0	0.13588278022620878	6.642314675862161E-4
31	0.0	4.744510482758686E-5	0.0	0.44902047208828205	6.642314675862161E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1040	0.0	21.346155	37
TACTTAC	650	0.0	20.776922	31
GGTATCA	955	0.0	19.371727	1
GTACTTA	725	0.0	18.88276	30
TCTTATA	2025	0.0	17.814814	37
TATACCG	95	3.6092733E-6	17.526316	5
TTTCGTA	65	0.0015805245	17.076925	16
TTACATA	435	0.0	17.011496	34
ATATACT	180	1.0913936E-11	16.444445	4
GTATTAG	170	8.54925E-11	16.32353	1
ACTTACA	655	0.0	15.816793	32
TATATAC	190	2.7284841E-11	15.578948	3
CATATAA	315	0.0	15.269842	37
TACATAT	400	0.0	15.262499	35
ACGACCG	75	0.004105916	14.8	5
GTATAGA	165	1.6470949E-8	14.575757	1
GTCTTAG	305	0.0	14.557377	1
AGTGTAC	995	0.0	14.502513	27
CTTACAT	580	0.0	14.353448	33
TATTAGG	155	1.2115197E-7	14.322581	2
>>END_MODULE