FastQCFastQC Report
Thu 9 Feb 2017
SRR2727521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287005
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9750.021.25128237
AATACCG450.003826052620.5555575
CGTTACG5000.019.9824
CTCGTTA7100.018.76056522
TACTTAC4950.017.93939231
GTACTTA4950.017.93939230
TCGTTAC6500.017.64615423
TCTCGTT7750.017.18709821
GTACCGT650.001580073117.0769236
AGTGTAC5900.016.93220327
GTTACGG9050.016.3535925
GCTTATA1708.54925E-1116.323531
TACGGAT10800.016.2731527
CTATACT803.3829594E-416.18754
CTATATG700.002592719615.8571431
ACGGATG11200.015.85714328
TCTTATA16750.015.79402937
TACATAT2600.015.65384635
GATCTCG9200.015.48369619
TCGTTAT1201.9358395E-615.41666723
Produced by FastQC (version 0.11.5)