##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727521.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1287005
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.569609286677206	34.0	31.0	34.0	31.0	34.0
2	32.74412686819399	34.0	31.0	34.0	31.0	34.0
3	32.83930987059102	34.0	31.0	34.0	31.0	34.0
4	36.296222625397725	37.0	37.0	37.0	35.0	37.0
5	36.150978434427216	37.0	35.0	37.0	35.0	37.0
6	36.17053779899845	37.0	36.0	37.0	35.0	37.0
7	36.146842475359456	37.0	35.0	37.0	35.0	37.0
8	36.137549582169456	37.0	35.0	37.0	35.0	37.0
9	37.853612068329184	39.0	38.0	39.0	35.0	39.0
10	37.82046612095524	39.0	37.0	39.0	35.0	39.0
11	37.90026456773672	39.0	38.0	39.0	35.0	39.0
12	37.878179960450815	39.0	38.0	39.0	35.0	39.0
13	37.90819771484959	39.0	38.0	39.0	35.0	39.0
14	39.25142792763043	40.0	39.0	41.0	36.0	41.0
15	39.26879071953877	40.0	39.0	41.0	36.0	41.0
16	39.24259190912234	40.0	39.0	41.0	36.0	41.0
17	39.26351723575277	40.0	39.0	41.0	36.0	41.0
18	39.23197190376106	40.0	39.0	41.0	36.0	41.0
19	39.28336020450581	40.0	39.0	41.0	36.0	41.0
20	39.25187703233476	40.0	39.0	41.0	36.0	41.0
21	39.235806387698574	40.0	39.0	41.0	36.0	41.0
22	39.21112505390422	40.0	39.0	41.0	36.0	41.0
23	39.16771418914456	40.0	39.0	41.0	36.0	41.0
24	39.126624993686896	40.0	39.0	41.0	36.0	41.0
25	39.06299897824795	40.0	39.0	41.0	36.0	41.0
26	38.96381599139087	40.0	38.0	41.0	35.0	41.0
27	38.917126973088685	40.0	38.0	41.0	35.0	41.0
28	38.8361342807526	40.0	38.0	41.0	35.0	41.0
29	38.761012583478696	40.0	38.0	41.0	35.0	41.0
30	38.704418397752924	40.0	38.0	41.0	35.0	41.0
31	38.630818062089894	40.0	38.0	41.0	35.0	41.0
32	38.5466233619916	40.0	38.0	41.0	35.0	41.0
33	38.50183332621086	40.0	38.0	41.0	34.0	41.0
34	38.461263942253524	40.0	38.0	41.0	34.0	41.0
35	38.454690541217786	40.0	38.0	41.0	34.0	41.0
36	38.381613902043895	40.0	38.0	41.0	34.0	41.0
37	38.380725016608324	40.0	38.0	41.0	34.0	41.0
38	38.34798543906201	40.0	38.0	41.0	34.0	41.0
39	38.290683408378364	40.0	38.0	41.0	34.0	41.0
40	38.24908450239121	40.0	38.0	41.0	34.0	41.0
41	38.198191926216296	40.0	38.0	41.0	34.0	41.0
42	38.16703198511272	40.0	38.0	41.0	34.0	41.0
43	37.05976511357765	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	1.0
14	0.0
15	1.0
16	3.0
17	4.0
18	16.0
19	29.0
20	59.0
21	140.0
22	254.0
23	484.0
24	806.0
25	1389.0
26	2250.0
27	3320.0
28	4941.0
29	7060.0
30	9870.0
31	13762.0
32	18790.0
33	25661.0
34	37357.0
35	54192.0
36	82741.0
37	139915.0
38	329361.0
39	554595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23091207881865	16.418040333953638	12.238025493296451	28.11302209393126
2	18.28703074191631	19.440406214428073	35.82635654096138	26.446206502694242
3	19.982517550436867	21.160368452337014	29.74324109074945	29.11387290647666
4	15.652930641295098	14.409112629710064	32.760167986915356	37.17778874207948
5	16.360387100283216	35.32589228480076	32.79000470083644	15.523715914079588
6	37.45750793508961	32.280760370006334	14.643455153631882	15.618276541272177
7	30.638731007261043	30.389081627499504	19.860373502822444	19.11181386241701
8	28.072385111168952	34.95052466773633	18.858434893415332	18.11865532767938
9	29.52552631885657	13.901655393724187	17.07064075120143	39.50217753621781
10	17.036996748264382	26.43696022936974	31.93592876484551	24.59011425752037
11	38.157660615149126	20.742032859235202	19.723311098247482	21.376995427368193
12	22.598669002839927	23.056242982738993	28.970905318938158	25.374182695482922
13	32.3625782339618	18.638156028919855	22.626485522589267	26.37278021452908
14	23.44225546909297	19.084618940874357	24.13681376529229	33.336311824740385
15	27.554749204548546	25.76027288161274	20.990205943255855	25.694771970582863
16	25.523133165760814	25.1084494621233	23.566963609309987	25.80145376280589
17	25.680009013174	24.331995602192688	23.651112466540532	26.33688291809278
18	25.500988729647517	25.346133076406076	24.202236976546324	24.950641217400086
19	27.47137734507636	24.304567581322527	24.043574034288913	24.180481039312202
20	25.838594255655572	23.58926344497496	24.03914514706625	26.532997152303217
21	25.318938154863424	24.893687281712193	25.099980186557165	24.68739437686722
22	26.713882230449766	23.38662242959429	23.132000264179236	26.767495075776704
23	25.486070372686974	24.032385266568507	24.54232889538114	25.93921546536338
24	24.96330628086138	23.291517903970846	24.800680650036323	26.94449516513145
25	25.966954285336886	22.973570421249335	23.952509897008948	27.10696539640483
26	25.284672553719684	23.681959277547485	24.91676411513553	26.116604053597303
27	26.71543622596649	24.139067058791536	23.77683070384342	25.368666011398556
28	25.47993209039592	25.564391746729807	22.63145830824278	26.324217854631488
29	25.61054541357648	24.88545110547356	24.350410449065855	25.153593031884103
30	27.347368502841867	23.82888955365364	23.136895350056914	25.686846593447576
31	25.857630700735427	24.049945415907477	24.499982517550436	25.59244136580666
32	25.368277512519377	24.21987482566113	23.946293914942054	26.46555374687744
33	25.28156456268624	23.345674647728643	24.189416513533356	27.183344276051763
34	25.907280857494726	23.86859413910591	25.30130030574862	24.922824697650746
35	25.859184696252154	23.33300958426735	24.450254661015304	26.357551058465194
36	25.118006534551146	23.252668016052773	25.52165687001993	26.10766857937615
37	24.291125520102874	23.132544162610092	26.211164680789896	26.36516563649714
38	25.6589523739224	22.185772394046644	25.419403965019562	26.735871267011397
39	23.71164059191689	23.005815828221333	27.24418320053147	26.038360379330307
40	25.774336541039077	21.670622880252992	26.857859915074144	25.697180663633784
41	24.2982738994798	22.615374454644698	26.99080423152979	26.09554741434571
42	23.724694154257364	22.629982012501895	27.600747471843544	26.044576361397198
43	23.046996709414493	22.15756737541812	26.886531132357682	27.908904782809703
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	33.5
2	45.0
3	114.0
4	183.0
5	183.0
6	236.5
7	290.0
8	268.5
9	247.0
10	327.0
11	407.0
12	407.0
13	769.5
14	1132.0
15	1636.0
16	2140.0
17	2012.5
18	1885.0
19	1885.0
20	2165.0
21	2445.0
22	2645.0
23	2845.0
24	3684.5
25	4524.0
26	4524.0
27	5941.5
28	7359.0
29	9094.5
30	10830.0
31	13725.0
32	16620.0
33	16620.0
34	20649.0
35	24678.0
36	29176.5
37	33675.0
38	39473.5
39	45272.0
40	45272.0
41	51964.5
42	58657.0
43	66750.5
44	74844.0
45	86418.5
46	97993.0
47	97993.0
48	110804.0
49	123615.0
50	132590.0
51	141565.0
52	142035.5
53	142506.0
54	142506.0
55	131641.5
56	120777.0
57	111054.5
58	101332.0
59	94506.5
60	87681.0
61	87681.0
62	76454.0
63	65227.0
64	54165.0
65	43103.0
66	35966.5
67	28830.0
68	28830.0
69	23594.5
70	18359.0
71	14815.0
72	11271.0
73	9145.5
74	7020.0
75	7020.0
76	5749.0
77	4478.0
78	3604.5
79	2731.0
80	2027.5
81	1324.0
82	1324.0
83	979.5
84	635.0
85	460.5
86	286.0
87	200.5
88	115.0
89	115.0
90	76.5
91	38.0
92	23.5
93	9.0
94	9.0
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1287005.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.69836013491086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.3558012398733	58.63803573180687
2	8.168848299536325	11.223729647380013
3	2.6273401640903136	5.414818823687764
4	1.2539091863295053	3.4456601943574956
5	0.7072287527124992	2.4292727775803544
6	0.4526894659908344	1.8659414378351293
7	0.29334856833415923	1.4106795914736403
8	0.21760367086869412	1.1959212278412927
9	0.15808790881132836	0.9774342083246025
>10	0.7082266223512895	7.984183263275605
>50	0.031563100475772286	1.4891248369092602
>100	0.02354211477213624	3.036160006036466
>500	0.0015845426220667516	0.7109040363541418
>1k	2.2636323172382165E-4	0.17813421713740993
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.769977583614671E-5	0.0
3	0.0	0.0	0.0	1.5539955167229343E-4	0.0
4	0.0	0.0	0.0	1.5539955167229343E-4	0.0
5	0.0	0.0	0.0	1.5539955167229343E-4	0.0
6	0.0	0.0	0.0	1.5539955167229343E-4	7.769977583614671E-5
7	0.0	0.0	0.0	1.5539955167229343E-4	7.769977583614671E-5
8	0.0	0.0	0.0	1.5539955167229343E-4	1.5539955167229343E-4
9	0.0	0.0	0.0	3.8849887918073357E-4	2.3309932750844014E-4
10	0.0	0.0	0.0	4.661986550168803E-4	2.3309932750844014E-4
11	0.0	0.0	0.0	5.43898430853027E-4	2.3309932750844014E-4
12	0.0	0.0	0.0	6.215982066891737E-4	2.3309932750844014E-4
13	7.769977583614671E-5	0.0	0.0	7.769977583614671E-4	2.3309932750844014E-4
14	7.769977583614671E-5	0.0	0.0	8.546975341976138E-4	3.1079910334458685E-4
15	7.769977583614671E-5	0.0	0.0	9.323973100337606E-4	3.1079910334458685E-4
16	7.769977583614671E-5	0.0	0.0	0.001087796861706054	3.8849887918073357E-4
17	7.769977583614671E-5	0.0	0.0	0.0014762957408867875	4.661986550168803E-4
18	7.769977583614671E-5	0.0	0.0	0.001631695292559081	4.661986550168803E-4
19	7.769977583614671E-5	0.0	0.0	0.0018647946200675211	4.661986550168803E-4
20	7.769977583614671E-5	7.769977583614671E-5	0.0	0.0022532934992482547	4.661986550168803E-4
21	7.769977583614671E-5	7.769977583614671E-5	0.0	0.002719492154265135	4.661986550168803E-4
22	7.769977583614671E-5	7.769977583614671E-5	0.0	0.003263390585118162	4.661986550168803E-4
23	7.769977583614671E-5	7.769977583614671E-5	0.0	0.004040388343479629	5.43898430853027E-4
24	7.769977583614671E-5	7.769977583614671E-5	0.0	0.005594383860202563	6.215982066891737E-4
25	7.769977583614671E-5	7.769977583614671E-5	0.0	0.006526781170236324	6.215982066891737E-4
26	7.769977583614671E-5	7.769977583614671E-5	0.0	0.009090873772829165	6.215982066891737E-4
27	7.769977583614671E-5	7.769977583614671E-5	0.0	0.020201941717398145	6.992979825253204E-4
28	7.769977583614671E-5	7.769977583614671E-5	0.0	0.06316991775478728	6.992979825253204E-4
29	7.769977583614671E-5	7.769977583614671E-5	0.0	0.13504221040322298	6.992979825253204E-4
30	7.769977583614671E-5	7.769977583614671E-5	0.0	0.22734954409656527	6.992979825253204E-4
31	7.769977583614671E-5	7.769977583614671E-5	0.0	0.6702382663626015	7.769977583614671E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	975	0.0	21.251282	37
AATACCG	45	0.0038260526	20.555557	5
CGTTACG	500	0.0	19.98	24
CTCGTTA	710	0.0	18.760565	22
TACTTAC	495	0.0	17.939392	31
GTACTTA	495	0.0	17.939392	30
TCGTTAC	650	0.0	17.646154	23
TCTCGTT	775	0.0	17.187098	21
GTACCGT	65	0.0015800731	17.076923	6
AGTGTAC	590	0.0	16.932203	27
GTTACGG	905	0.0	16.35359	25
GCTTATA	170	8.54925E-11	16.32353	1
TACGGAT	1080	0.0	16.27315	27
CTATACT	80	3.3829594E-4	16.1875	4
CTATATG	70	0.0025927196	15.857143	1
ACGGATG	1120	0.0	15.857143	28
TCTTATA	1675	0.0	15.794029	37
TACATAT	260	0.0	15.653846	35
GATCTCG	920	0.0	15.483696	19
TCGTTAT	120	1.9358395E-6	15.416667	23
>>END_MODULE