FastQCFastQC Report
Thu 9 Feb 2017
SRR2727519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523959
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTA16570.10872995927055781No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC16030.10518655685618838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC7650.021.76470631
CTTATAC11550.021.3030337
GTACTTA8750.020.08571430
AGTGTAC10150.017.49753827
TTACATA4250.016.97646934
GTGTACT9900.016.63131328
CTACAGT11900.016.4789923
ACTTACT3500.015.85714332
ACTTACA6650.015.85714332
GTGTTAC700.002593013715.8571421
TAGTACT1302.5903864E-715.6538464
CTTACAT5450.015.61467833
CAGTGTA12200.015.31557326
TCTTATA19350.015.29715837
CTATAGT1101.4524072E-515.1363644
ACAGTGT12400.014.91935425
TACAGTG13150.014.77186324
GCTACAG12850.014.68482422
ACATATA3150.014.6825436
TGTACTT12050.014.58506229
Produced by FastQC (version 0.11.5)