##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727519.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523959
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40012821867255	33.0	31.0	34.0	31.0	34.0
2	32.58483725612041	34.0	31.0	34.0	31.0	34.0
3	32.67354633556415	34.0	31.0	34.0	31.0	34.0
4	36.182621710951544	37.0	35.0	37.0	35.0	37.0
5	36.011190589773086	37.0	35.0	37.0	35.0	37.0
6	36.022977652285924	37.0	35.0	37.0	35.0	37.0
7	36.0039036483265	37.0	35.0	37.0	35.0	37.0
8	35.98840388750616	37.0	35.0	37.0	35.0	37.0
9	37.680259114582476	39.0	37.0	39.0	35.0	39.0
10	37.63478282552221	39.0	37.0	39.0	35.0	39.0
11	37.7112940702473	39.0	37.0	39.0	35.0	39.0
12	37.687609706035396	39.0	37.0	39.0	35.0	39.0
13	37.72329636164753	39.0	37.0	39.0	35.0	39.0
14	39.02464830090573	40.0	38.0	41.0	36.0	41.0
15	39.029639248824935	40.0	38.0	41.0	36.0	41.0
16	39.000072836605185	40.0	38.0	41.0	36.0	41.0
17	39.026720535132505	40.0	38.0	41.0	36.0	41.0
18	38.987459636381296	40.0	38.0	41.0	36.0	41.0
19	39.03923071421213	40.0	39.0	41.0	36.0	41.0
20	39.00940904578142	40.0	38.0	41.0	36.0	41.0
21	38.98911125561777	40.0	38.0	41.0	36.0	41.0
22	38.96779244061028	40.0	38.0	41.0	36.0	41.0
23	38.919306228054694	40.0	38.0	41.0	35.0	41.0
24	38.87891209671651	40.0	38.0	41.0	35.0	41.0
25	38.806717241080634	40.0	38.0	41.0	35.0	41.0
26	38.69209342246084	40.0	38.0	41.0	35.0	41.0
27	38.64479818682786	40.0	38.0	41.0	35.0	41.0
28	38.55892382931562	40.0	38.0	41.0	35.0	41.0
29	38.48027276324363	40.0	38.0	41.0	34.0	41.0
30	38.430007631438905	40.0	38.0	41.0	34.0	41.0
31	38.3483039898055	40.0	38.0	41.0	34.0	41.0
32	38.26312846999165	40.0	38.0	41.0	34.0	41.0
33	38.22126841995093	40.0	38.0	41.0	34.0	41.0
34	38.17342198838683	40.0	38.0	41.0	34.0	41.0
35	38.1732303821822	40.0	38.0	41.0	34.0	41.0
36	38.09739304010147	40.0	38.0	41.0	34.0	41.0
37	38.09186795707758	40.0	38.0	41.0	34.0	41.0
38	38.06706545254826	40.0	38.0	41.0	33.0	41.0
39	38.005450277861804	40.0	38.0	41.0	33.0	41.0
40	37.965039085697185	40.0	37.0	41.0	33.0	41.0
41	37.911262048388444	40.0	37.0	41.0	33.0	41.0
42	37.87909976580735	40.0	37.0	41.0	33.0	41.0
43	36.78103610398968	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	5.0
17	4.0
18	17.0
19	43.0
20	91.0
21	233.0
22	389.0
23	721.0
24	1291.0
25	2060.0
26	3247.0
27	4920.0
28	7071.0
29	10135.0
30	14179.0
31	19727.0
32	26460.0
33	35741.0
34	50749.0
35	72226.0
36	109220.0
37	184793.0
38	430703.0
39	549930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.302608534744046	16.41041524082997	12.194685027615572	28.092291196810415
2	19.011075757287433	19.738654386371287	34.98348708856341	26.266782767777876
3	20.49425214195395	21.19335231459639	29.645941918384942	28.666453625064715
4	16.367763174731078	14.469549377640737	32.225932587425255	36.93675486020293
5	16.624331756956717	35.1561951469823	32.49142529425004	15.728047801810941
6	37.644451064628385	31.700459133086913	14.5464543337452	16.108635468539507
7	30.352063277292896	30.223057181984554	19.88609929794699	19.538780242775562
8	28.092094341120728	34.240488097120725	19.15537097782814	18.51204658393041
9	29.65388176453566	13.683176515903641	17.158073150261917	39.50486856929878
10	17.182811348599273	26.117631773558216	32.32396672088947	24.37559015695304
11	38.07084048849083	20.509278792933404	19.621197158191265	21.7986835603845
12	22.504279970786616	22.980473884139926	28.553983407690104	25.961262737383358
13	32.45382585751979	18.687904333384296	22.523112498433356	26.335157310662556
14	23.648995806317625	19.450063945289866	23.748407929609655	33.15253231878286
15	28.049639130711522	25.92307273358404	20.744586960672827	25.28270117503161
16	25.435854901608245	25.088929557816186	23.744930145758513	25.730285394817052
17	25.861128809895806	23.90011804779525	23.465526303529163	26.773226838779784
18	25.252844728762387	25.353175511939625	24.297569685273686	25.0964100740243
19	27.545754183675548	24.301966129010033	24.03378306109285	24.11849662622157
20	25.814605248566398	23.353843508913297	23.88673186089652	26.944819381623788
21	25.37161432820699	24.85224340024896	25.441366860919484	24.334775410624566
22	26.944031958865033	23.002390484258434	22.984345379370442	27.069232177506088
23	25.572013420308554	24.03942625753055	24.612670025899646	25.77589029626125
24	24.88629943456484	23.354762168798505	24.805194890413716	26.95374350622294
25	26.066843005618917	22.822333146757888	23.80477427542342	27.306049572199775
26	25.419319023674518	23.672093540574256	24.841088244500014	26.06749919125121
27	27.096267025556465	24.073154199030288	23.592760697630315	25.23781807778293
28	25.24575792393365	25.565320326859187	22.400077692378865	26.7888440568283
29	25.691636061075133	25.142999253916937	24.21823684232975	24.94712784267818
30	27.73788533681024	23.450565271112936	22.941627694708323	25.869921697368497
31	26.04505764262687	24.044347649772728	24.436156090813466	25.474438616786934
32	25.62063677566129	24.057208888165626	23.896246552564733	26.42590778360835
33	25.455343614887276	23.215322721936747	24.08706533443485	27.242268328741126
34	26.258711684500696	23.755822827254537	25.230468798701278	24.754996689543486
35	26.109757545970723	23.10016214346974	24.259576537164058	26.53050377339548
36	25.333489942970903	23.03782450840213	25.467679904774343	26.161005643852626
37	24.394553921726242	23.160924933019853	26.348674734687744	26.095846410566164
38	26.022288001186382	21.821256346135296	25.462364801152788	26.69409085152553
39	23.808580152090705	22.949305066606122	27.21772698609346	26.024387795209712
40	26.13187100177892	21.427479348197686	26.743632866763477	25.69701678325992
41	24.390748045058956	22.626330498392676	26.77526101423988	26.207660442308487
42	23.995199345914163	22.702513650301615	27.382495198361635	25.919791805422587
43	23.272870201888633	22.238787263961825	26.414424535043267	28.073917999106275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	62.5
2	90.0
3	149.5
4	209.0
5	209.0
6	260.5
7	312.0
8	303.5
9	295.0
10	404.5
11	514.0
12	514.0
13	815.0
14	1116.0
15	1544.5
16	1973.0
17	1879.5
18	1786.0
19	1786.0
20	2196.5
21	2607.0
22	2939.5
23	3272.0
24	4279.5
25	5287.0
26	5287.0
27	6843.0
28	8399.0
29	10264.0
30	12129.0
31	15388.5
32	18648.0
33	18648.0
34	22840.0
35	27032.0
36	32146.0
37	37260.0
38	44023.5
39	50787.0
40	50787.0
41	58456.0
42	66125.0
43	75834.5
44	85544.0
45	100206.5
46	114869.0
47	114869.0
48	131352.0
49	147835.0
50	160802.5
51	173770.0
52	174098.0
53	174426.0
54	174426.0
55	160502.0
56	146578.0
57	133573.0
58	120568.0
59	112430.5
60	104293.0
61	104293.0
62	90818.0
63	77343.0
64	64363.0
65	51383.0
66	42796.5
67	34210.0
68	34210.0
69	27917.5
70	21625.0
71	17645.5
72	13666.0
73	11084.5
74	8503.0
75	8503.0
76	6930.0
77	5357.0
78	4260.5
79	3164.0
80	2416.5
81	1669.0
82	1669.0
83	1197.5
84	726.0
85	546.0
86	366.0
87	244.0
88	122.0
89	122.0
90	83.5
91	45.0
92	29.0
93	13.0
94	8.5
95	4.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1523959.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.92027151352127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.36744563459288	54.771574775000154
2	8.507970798283768	11.04679548507385
3	2.7217382821059037	5.300879647891807
4	1.3412851620482853	3.483063875889282
5	0.7757822235748392	2.5181996294920914
6	0.5128090823540605	1.9975022916615182
7	0.3494560491511636	1.588074711505518
8	0.2700947077363009	1.402769740848466
9	0.1956613289332772	1.1432147939140278
>10	0.8868493958560871	9.726541951403842
>50	0.03815651328488044	1.7380054454562854
>100	0.030013241680793976	3.826432000855691
>500	0.002230621064863075	1.0175712848525202
>1k	5.069593329234261E-4	0.4393743661550277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTA	1657	0.10872995927055781	No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC	1603	0.10518655685618838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.561856322906325E-5	0.0
3	0.0	0.0	0.0	6.561856322906325E-5	0.0
4	0.0	0.0	0.0	6.561856322906325E-5	0.0
5	0.0	0.0	0.0	6.561856322906325E-5	0.0
6	6.561856322906325E-5	0.0	0.0	6.561856322906325E-5	6.561856322906325E-5
7	6.561856322906325E-5	0.0	0.0	6.561856322906325E-5	6.561856322906325E-5
8	6.561856322906325E-5	0.0	0.0	6.561856322906325E-5	6.561856322906325E-5
9	6.561856322906325E-5	0.0	0.0	6.561856322906325E-5	1.9685568968718975E-4
10	6.561856322906325E-5	0.0	0.0	6.561856322906325E-5	1.9685568968718975E-4
11	6.561856322906325E-5	0.0	0.0	1.312371264581265E-4	1.9685568968718975E-4
12	6.561856322906325E-5	0.0	0.0	1.312371264581265E-4	1.9685568968718975E-4
13	6.561856322906325E-5	0.0	0.0	1.312371264581265E-4	1.9685568968718975E-4
14	6.561856322906325E-5	0.0	0.0	1.9685568968718975E-4	1.9685568968718975E-4
15	6.561856322906325E-5	0.0	0.0	4.5932994260344275E-4	2.62474252916253E-4
16	6.561856322906325E-5	0.0	0.0	5.24948505832506E-4	3.2809281614531625E-4
17	6.561856322906325E-5	0.0	0.0	6.561856322906325E-4	3.2809281614531625E-4
18	6.561856322906325E-5	0.0	0.0	7.87422758748759E-4	3.2809281614531625E-4
19	6.561856322906325E-5	0.0	0.0	9.186598852068855E-4	3.2809281614531625E-4
20	6.561856322906325E-5	0.0	0.0	0.0013779898278103281	3.2809281614531625E-4
21	6.561856322906325E-5	0.0	0.0	0.0019029383336428341	3.2809281614531625E-4
22	6.561856322906325E-5	0.0	0.0	0.0022310311497881503	3.2809281614531625E-4
23	6.561856322906325E-5	0.0	0.0	0.003084072471765973	5.24948505832506E-4
24	6.561856322906325E-5	0.0	0.0	0.0045276808628053646	5.24948505832506E-4
25	6.561856322906325E-5	0.0	0.0	0.005840052127386629	6.561856322906325E-4
26	6.561856322906325E-5	0.0	0.0	0.008530413219778222	7.218041955196957E-4
27	6.561856322906325E-5	0.0	0.0	0.022507167187568695	7.218041955196957E-4
28	6.561856322906325E-5	0.0	0.0	0.06798083150530952	7.218041955196957E-4
29	6.561856322906325E-5	0.0	0.0	0.13576480732093185	7.218041955196957E-4
30	6.561856322906325E-5	0.0	0.0	0.22474357905954162	7.218041955196957E-4
31	6.561856322906325E-5	0.0	0.0	0.6437181052771105	7.218041955196957E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTAC	765	0.0	21.764706	31
CTTATAC	1155	0.0	21.30303	37
GTACTTA	875	0.0	20.085714	30
AGTGTAC	1015	0.0	17.497538	27
TTACATA	425	0.0	16.976469	34
GTGTACT	990	0.0	16.631313	28
CTACAGT	1190	0.0	16.47899	23
ACTTACT	350	0.0	15.857143	32
ACTTACA	665	0.0	15.857143	32
GTGTTAC	70	0.0025930137	15.857142	1
TAGTACT	130	2.5903864E-7	15.653846	4
CTTACAT	545	0.0	15.614678	33
CAGTGTA	1220	0.0	15.315573	26
TCTTATA	1935	0.0	15.297158	37
CTATAGT	110	1.4524072E-5	15.136364	4
ACAGTGT	1240	0.0	14.919354	25
TACAGTG	1315	0.0	14.771863	24
GCTACAG	1285	0.0	14.684824	22
ACATATA	315	0.0	14.68254	36
TGTACTT	1205	0.0	14.585062	29
>>END_MODULE