FastQCFastQC Report
Thu 9 Feb 2017
SRR2727518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2467659
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.2768615923026642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53200.21558894482584506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48470.19642098037046446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.10649769680494753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG450.003827032920.5555578
CTTATAC16250.020.03692237
TATACTG3250.017.0769235
CGACTTA2300.015.28260719
TCTTATA28100.015.20818537
TATACCG1101.4531321E-515.1363645
GTCTTAG3150.014.6825391
TTATACT3450.013.9420284
TCGTTAC6800.013.87523
AGGGCGT7350.013.84353710
CTCGTTA7700.013.69480522
GACCGTG2850.013.6315797
ATAGCGT950.001245781713.6315796
GTACTTA7600.013.63157930
CGTTATG1254.8487847E-513.3224
GGGCGTC6950.013.30935311
GCGTATA850.00940872613.0588249
ATTAGAC1307.004157E-512.8076933
CTCTTAT44650.012.76147837
TACCGTG1755.311613E-712.6857157
Produced by FastQC (version 0.11.5)