##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727518.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2467659
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61180860078317	34.0	31.0	34.0	31.0	34.0
2	32.7764918086332	34.0	31.0	34.0	31.0	34.0
3	32.87123018212808	34.0	31.0	34.0	31.0	34.0
4	36.322122708202386	37.0	37.0	37.0	35.0	37.0
5	36.184927496060034	37.0	35.0	37.0	35.0	37.0
6	36.20764011559134	37.0	36.0	37.0	35.0	37.0
7	36.183800922250605	37.0	36.0	37.0	35.0	37.0
8	36.175650282312105	37.0	36.0	37.0	35.0	37.0
9	37.88942921205888	39.0	38.0	39.0	35.0	39.0
10	37.87067500007092	39.0	38.0	39.0	35.0	39.0
11	37.94340303907469	39.0	38.0	39.0	35.0	39.0
12	37.917982995219354	39.0	38.0	39.0	35.0	39.0
13	37.94890542007627	39.0	38.0	39.0	35.0	39.0
14	39.29592986713318	40.0	39.0	41.0	36.0	41.0
15	39.31521980954419	40.0	39.0	41.0	36.0	41.0
16	39.29837064197282	40.0	39.0	41.0	36.0	41.0
17	39.320656136038245	40.0	39.0	41.0	36.0	41.0
18	39.30165310523051	40.0	39.0	41.0	36.0	41.0
19	39.34187097974234	40.0	39.0	41.0	36.0	41.0
20	39.32054955729296	40.0	39.0	41.0	36.0	41.0
21	39.30128190321272	40.0	39.0	41.0	36.0	41.0
22	39.26636500424086	40.0	39.0	41.0	36.0	41.0
23	39.2200368851612	40.0	39.0	41.0	36.0	41.0
24	39.18442864269334	40.0	39.0	41.0	36.0	41.0
25	39.1218888023021	40.0	39.0	41.0	36.0	41.0
26	39.01963845085565	40.0	39.0	41.0	36.0	41.0
27	38.97103489582637	40.0	38.0	41.0	35.0	41.0
28	38.901698330279835	40.0	38.0	41.0	35.0	41.0
29	38.82129094822259	40.0	38.0	41.0	35.0	41.0
30	38.75319482959355	40.0	38.0	41.0	35.0	41.0
31	38.671503234442035	40.0	38.0	41.0	35.0	41.0
32	38.571022171215716	40.0	38.0	41.0	35.0	41.0
33	38.518773055758516	40.0	38.0	41.0	35.0	41.0
34	38.46472304317574	40.0	38.0	41.0	35.0	41.0
35	38.44381780464805	40.0	38.0	41.0	35.0	41.0
36	38.37777423866101	40.0	38.0	41.0	34.0	41.0
37	38.3537348555858	40.0	38.0	41.0	34.0	41.0
38	38.29876575329087	40.0	38.0	41.0	34.0	41.0
39	38.242546073019	40.0	38.0	41.0	34.0	41.0
40	38.172395375536084	40.0	38.0	41.0	34.0	41.0
41	38.11416407210235	40.0	38.0	41.0	34.0	41.0
42	38.054312204401015	40.0	38.0	41.0	34.0	41.0
43	36.97613406066235	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	4.0
13	1.0
14	2.0
15	5.0
16	6.0
17	15.0
18	19.0
19	46.0
20	110.0
21	241.0
22	450.0
23	923.0
24	1545.0
25	2534.0
26	4091.0
27	6249.0
28	9239.0
29	13338.0
30	18667.0
31	25782.0
32	34714.0
33	48198.0
34	69363.0
35	101810.0
36	156219.0
37	274281.0
38	621777.0
39	1078023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.489841991944594	17.056773241359522	12.36743002173315	27.085954744962738
2	18.009862788983405	20.0910255428323	35.88088953943799	26.018222128746316
3	19.863157753968437	21.837660714061382	29.86988882985858	28.429292702111596
4	15.14123304719169	14.785592336704545	33.67624943316723	36.39692518293654
5	15.903129241114758	35.385926499569024	33.3378315237235	15.373112735592724
6	36.24033952827356	33.26922398921407	14.92844027477054	15.561996207741831
7	29.986841780002827	30.32185565347562	20.590284151902676	19.101018414618874
8	27.560331472055093	34.712575765128	19.302221255043747	18.424871507773158
9	28.21852614157791	14.359115258631764	18.19380230412711	39.22855629566322
10	16.89398737832091	26.581833227362452	32.60961097137003	23.914568422946605
11	36.89071301991078	21.210588659129968	20.66638867039571	21.23230965056355
12	22.232002071599034	23.88486415667643	29.676750312745803	24.206383458978735
13	31.601084266505218	19.268667186187393	23.600667677341157	25.529580869966228
14	22.98084946096685	19.67508476657431	25.181193998036193	32.16287177442264
15	26.39088301908813	26.6235326680064	21.94152433541263	25.04405997749284
16	24.967590740860064	25.427216645411704	24.36276649245297	25.242426121275262
17	24.73745359468225	25.196917402282892	24.96195787181292	25.103671131221937
18	24.78563691336607	25.219408354233707	25.537239950900837	24.45771478149939
19	25.984424914463467	24.858864210978908	25.528324618596006	23.628386255961622
20	25.07988340366315	24.37002033101008	25.428270275593185	25.121825989733587
21	24.679058168085625	25.309615307463467	25.912372819745354	24.098953704705554
22	25.59912046194389	24.343720100710836	24.751596553656725	25.30556288368855
23	24.8695626097447	24.612882087841147	25.42437994876926	25.09317535364489
24	24.458930508631866	24.35547212965811	25.669389490201038	25.51620787150899
25	25.073642671049768	24.07103250489634	25.13228124307289	25.723043580981003
26	24.639344415091387	24.596024004937473	25.72215204775052	25.042479532220618
27	25.3392790494959	24.91936689793849	25.127337286067487	24.614016766498125
28	24.820163563928404	25.608157366961965	24.478341618513742	25.093337450595886
29	24.7218517631488	25.41988175837909	25.498174585710586	24.36009189276152
30	25.681465713050304	24.798361524019324	24.779598801941436	24.740573960988936
31	24.86640171920026	24.775546378166514	25.499917127933802	24.858134774699423
32	24.420432482770106	24.99060850790162	25.35062583606568	25.238333173262596
33	24.47477548559181	24.449083118858805	25.44488521307036	25.631256182479024
34	24.987163947692935	24.6464361566975	26.115763969008682	24.25063592660088
35	24.76748205485442	24.445557510174623	25.6141954783866	25.17276495658436
36	24.3507713180792	24.30048073903242	26.1926789722567	25.15606897063168
37	23.76385878275726	24.066250644841933	26.68209019155402	25.487800380846785
38	24.43279237528362	23.599127756306686	26.435459680612272	25.532620187797423
39	23.370125288785847	23.97612474008767	27.480336626738133	25.173413344388347
40	24.49175514120873	23.242595512589055	27.442000697827375	24.823648648374835
41	23.5233069074779	23.679487319763385	27.622981943615386	25.174223829143326
42	23.064856205820984	23.852850008854546	27.86296647956626	25.21932730575821
43	22.724817326867285	23.498465549737627	27.548295773443577	26.228421349951514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	296.0
1	350.0
2	404.0
3	949.5
4	1495.0
5	1495.0
6	1877.5
7	2260.0
8	2032.5
9	1805.0
10	2485.5
11	3166.0
12	3166.0
13	5481.5
14	7797.0
15	11000.5
16	14204.0
17	12982.5
18	11761.0
19	11761.0
20	13057.0
21	14353.0
22	13229.0
23	12105.0
24	14774.0
25	17443.0
26	17443.0
27	21035.5
28	24628.0
29	28527.5
30	32427.0
31	38757.5
32	45088.0
33	45088.0
34	52949.5
35	60811.0
36	69878.5
37	78946.0
38	89370.5
39	99795.0
40	99795.0
41	112075.0
42	124355.0
43	137957.5
44	151560.0
45	167517.0
46	183474.0
47	183474.0
48	201614.0
49	219754.0
50	230658.0
51	241562.0
52	242655.5
53	243749.0
54	243749.0
55	228166.5
56	212584.0
57	195663.0
58	178742.0
59	165909.5
60	153077.0
61	153077.0
62	133876.5
63	114676.0
64	96613.5
65	78551.0
66	65340.5
67	52130.0
68	52130.0
69	42713.0
70	33296.0
71	27026.5
72	20757.0
73	16917.0
74	13077.0
75	13077.0
76	10543.0
77	8009.0
78	6417.0
79	4825.0
80	3717.5
81	2610.0
82	2610.0
83	1906.0
84	1202.0
85	887.5
86	573.0
87	391.0
88	209.0
89	209.0
90	136.5
91	64.0
92	43.0
93	22.0
94	17.0
95	12.0
96	12.0
97	8.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2467659.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.7356895412469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41083882403319	51.70100799298283
2	9.50066982178995	11.920621447474158
3	3.024151226474308	5.691666374096196
4	1.4866098052084116	3.730539648341137
5	0.8947301217596866	2.8065755570958864
6	0.5781446729500224	2.176218282727099
7	0.3932320689856299	1.7268779498281266
8	0.3026571254224757	1.5189922766360526
9	0.2169418290460253	1.224899571198753
>10	1.1312159549104324	11.945620784266044
>50	0.040866072582326045	1.7372624780619745
>100	0.01780963908042478	2.1131356255779954
>500	0.0014865232852179777	0.6191118994736258
>1k	5.17051577463658E-4	0.594743162055888
>5k	1.292628943659145E-4	0.4927269501842889
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6832	0.2768615923026642	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5320	0.21558894482584506	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4847	0.19642098037046446	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2628	0.10649769680494753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.0524237749218994E-5	0.0
4	0.0	0.0	0.0	8.104847549843799E-5	0.0
5	0.0	0.0	0.0	8.104847549843799E-5	0.0
6	0.0	0.0	0.0	8.104847549843799E-5	0.0
7	0.0	0.0	0.0	8.104847549843799E-5	0.0
8	0.0	0.0	0.0	8.104847549843799E-5	0.0
9	0.0	0.0	0.0	8.104847549843799E-5	4.0524237749218994E-5
10	0.0	0.0	0.0	8.104847549843799E-5	4.0524237749218994E-5
11	4.0524237749218994E-5	0.0	0.0	1.6209695099687598E-4	4.0524237749218994E-5
12	4.0524237749218994E-5	0.0	0.0	1.6209695099687598E-4	4.0524237749218994E-5
13	4.0524237749218994E-5	0.0	0.0	1.6209695099687598E-4	4.0524237749218994E-5
14	4.0524237749218994E-5	4.0524237749218994E-5	0.0	1.6209695099687598E-4	2.0262118874609498E-4
15	4.0524237749218994E-5	4.0524237749218994E-5	0.0	1.6209695099687598E-4	2.0262118874609498E-4
16	4.0524237749218994E-5	4.0524237749218994E-5	0.0	3.2419390199375195E-4	2.4314542649531398E-4
17	4.0524237749218994E-5	4.0524237749218994E-5	0.0	5.26815090739847E-4	2.4314542649531398E-4
18	4.0524237749218994E-5	4.0524237749218994E-5	0.0	7.699605172351609E-4	2.83669664244533E-4
19	4.0524237749218994E-5	4.0524237749218994E-5	0.0	8.91533230482818E-4	2.83669664244533E-4
20	4.0524237749218994E-5	4.0524237749218994E-5	0.0	0.001134678656978132	2.83669664244533E-4
21	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.0016209695099687598	2.83669664244533E-4
22	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.0024719785027023586	2.83669664244533E-4
23	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.0032014147821883008	4.0524237749218995E-4
24	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.004781860054407842	4.0524237749218995E-4
25	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.006281256851128944	4.0524237749218995E-4
26	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.009077429255825055	4.0524237749218995E-4
27	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.02018107039911106	4.0524237749218995E-4
28	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.06248837460929569	4.0524237749218995E-4
29	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.1280160670497828	4.0524237749218995E-4
30	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.21259015123240285	4.0524237749218995E-4
31	8.104847549843799E-5	4.0524237749218994E-5	0.0	0.5973677886612372	4.0524237749218995E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAACG	45	0.0038270329	20.555557	8
CTTATAC	1625	0.0	20.036922	37
TATACTG	325	0.0	17.076923	5
CGACTTA	230	0.0	15.282607	19
TCTTATA	2810	0.0	15.208185	37
TATACCG	110	1.4531321E-5	15.136364	5
GTCTTAG	315	0.0	14.682539	1
TTATACT	345	0.0	13.942028	4
TCGTTAC	680	0.0	13.875	23
AGGGCGT	735	0.0	13.843537	10
CTCGTTA	770	0.0	13.694805	22
GACCGTG	285	0.0	13.631579	7
ATAGCGT	95	0.0012457817	13.631579	6
GTACTTA	760	0.0	13.631579	30
CGTTATG	125	4.8487847E-5	13.32	24
GGGCGTC	695	0.0	13.309353	11
GCGTATA	85	0.009408726	13.058824	9
ATTAGAC	130	7.004157E-5	12.807693	3
CTCTTAT	4465	0.0	12.761478	37
TACCGTG	175	5.311613E-7	12.685715	7
>>END_MODULE