FastQCFastQC Report
Thu 9 Feb 2017
SRR2727517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869638
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC358.8714843E-426.428573
CTTATAC9000.018.08888837
TCTTATA16950.017.68141737
GTATACG852.7245944E-517.4117641
ATAGCGT650.001580434517.0769236
TATACTG2150.015.4883725
CTCGTTA7900.015.45569622
CGTTACG6050.014.98347124
GTTATAT1001.0939461E-414.7999991
TCGTTAC7300.014.19178123
TAGGACT3050.013.950824
ATCTCGT9050.013.69613320
GTTACGG9600.013.48958325
TTATATA1652.5973168E-713.4545452
TATACCG1254.8475536E-513.3200015
ATATTAG1254.8475536E-513.3200011
TCTCGTT8950.013.2290521
ATTATAC1857.389826E-813.03
GATCTCG9950.012.82914519
TACGGAT11450.012.76419227
Produced by FastQC (version 0.11.5)