FastQCFastQC Report
Thu 9 Feb 2017
SRR2727516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326445
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42780.1838857140400912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36750.1579663391999381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29930.1286512253674598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12400.022.2298437
GTTCTAG2400.015.4166671
GTATATA2950.015.0508471
GGTATCA28850.014.876951
CGTATAC1001.0941286E-414.7999993
GTACTTA7800.014.70512730
TCTTATA20250.014.43456837
CGTTACA2009.858923E-1013.87499924
TCGTTAC7950.013.72955923
TACTTAC8100.013.70370431
TTAGTAC1356.5734203E-613.7037043
CTCGTTA10150.013.48768522
TAGTATA1254.848551E-513.322
ATATGCC2358.094503E-1012.5957453
ATCTCGT11750.012.43829820
GTATACG1050.002644971512.3333341
TTAATAC2255.7134457E-912.3333333
AGTGTAC10050.012.33333327
CGTTACG6700.012.14925424
CTCTTAT29650.012.10455337
Produced by FastQC (version 0.11.5)