FastQCFastQC Report
Thu 9 Feb 2017
SRR2727515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912378
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT27390.1432248227076446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.14160380426882133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23380.12225616483770467No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG19350.10118292513300194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11000.019.5090937
TCTTATA20050.016.4239437
TAGGACG700.00259333815.8571424
TAGTACT1659.749783E-1015.696974
CAATAGT1907.1322575E-913.6315794
CGTTATC1102.457698E-413.45454514
GTCTTAC1805.163747E-813.3611111
CTAGATA1254.847668E-513.324
TACTTAC5550.012.66666731
CCCTATA1050.002644675812.3333332
AGTATCG2859.094947E-1212.33333310
CTAGGGT1807.4689524E-712.3333334
CTCTTAT29950.012.23038437
TCGTTAC5300.012.21698123
TAATACC2154.0159648E-812.0465124
GTAGAGT4150.012.0361446
TACGGAT7900.011.94303827
ATAGTAC1552.721897E-511.9354843
AATACCC1401.3992359E-411.8928575
GTTACGG6300.011.74603225
Produced by FastQC (version 0.11.5)