FastQCFastQC Report
Thu 9 Feb 2017
SRR2727514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425524
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC5200.023.12531
GTACTTA5900.022.26271230
CTTATAC9700.020.97938237
ACTTACA4850.019.83505232
ATAGTCT759.268331E-619.7333344
AGTGTAC7400.019.027
GTTAACC609.2383014E-418.53
GTGTACT7600.018.25657828
TACATAT3100.017.90322735
TTACATA3150.017.61904734
TATAAGT953.607618E-617.5263164
ACATATC1401.869921E-917.17857236
CATATAA2200.016.81818237
ACATATA2200.016.81818236
TAATACT1005.879785E-616.654
TGTACTT8450.016.63905329
GTATAAT904.447162E-516.4444451
CAGTGTA8550.016.2280726
CTTACAT3900.016.12820433
TAGAGTG1504.6729838E-916.0333335
Produced by FastQC (version 0.11.5)