##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727514.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1425524
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56235110738227	34.0	31.0	34.0	31.0	34.0
2	32.72940827372952	34.0	31.0	34.0	31.0	34.0
3	32.828310852711006	34.0	31.0	34.0	31.0	34.0
4	36.28686784649013	37.0	37.0	37.0	35.0	37.0
5	36.14819112129996	37.0	35.0	37.0	35.0	37.0
6	36.171101293278824	37.0	36.0	37.0	35.0	37.0
7	36.14364893190153	37.0	35.0	37.0	35.0	37.0
8	36.13621096523103	37.0	35.0	37.0	35.0	37.0
9	37.85816583936854	39.0	38.0	39.0	35.0	39.0
10	37.8191773691639	39.0	37.0	39.0	35.0	39.0
11	37.90510156265345	39.0	38.0	39.0	35.0	39.0
12	37.87470431925383	39.0	38.0	39.0	35.0	39.0
13	37.908477163485145	39.0	38.0	39.0	35.0	39.0
14	39.23498306587613	40.0	39.0	41.0	36.0	41.0
15	39.25776416251147	40.0	39.0	41.0	36.0	41.0
16	39.22802772875097	40.0	39.0	41.0	36.0	41.0
17	39.250466495127405	40.0	39.0	41.0	36.0	41.0
18	39.2218391272262	40.0	39.0	41.0	36.0	41.0
19	39.2702325600972	40.0	39.0	41.0	36.0	41.0
20	39.237575095193066	40.0	39.0	41.0	36.0	41.0
21	39.22137754257382	40.0	39.0	41.0	36.0	41.0
22	39.19499636624848	40.0	39.0	41.0	36.0	41.0
23	39.14807677738151	40.0	39.0	41.0	36.0	41.0
24	39.115709732000305	40.0	39.0	41.0	36.0	41.0
25	39.043744616014884	40.0	39.0	41.0	36.0	41.0
26	38.94504126202014	40.0	38.0	41.0	35.0	41.0
27	38.901232108333495	40.0	38.0	41.0	35.0	41.0
28	38.808109859953255	40.0	38.0	41.0	35.0	41.0
29	38.73537941136031	40.0	38.0	41.0	35.0	41.0
30	38.68617434711727	40.0	38.0	41.0	35.0	41.0
31	38.61398475227355	40.0	38.0	41.0	35.0	41.0
32	38.52811106652712	40.0	38.0	41.0	35.0	41.0
33	38.47973446957049	40.0	38.0	41.0	34.0	41.0
34	38.44127142019356	40.0	38.0	41.0	34.0	41.0
35	38.43049783798799	40.0	38.0	41.0	34.0	41.0
36	38.356219186769216	40.0	38.0	41.0	34.0	41.0
37	38.357838942031144	40.0	38.0	41.0	34.0	41.0
38	38.31849761912111	40.0	38.0	41.0	34.0	41.0
39	38.26052034199354	40.0	38.0	41.0	34.0	41.0
40	38.21836952587259	40.0	38.0	41.0	34.0	41.0
41	38.16584498051243	40.0	38.0	41.0	34.0	41.0
42	38.13043835109055	40.0	38.0	41.0	34.0	41.0
43	37.01433367659892	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	3.0
18	17.0
19	31.0
20	59.0
21	135.0
22	323.0
23	556.0
24	948.0
25	1519.0
26	2372.0
27	3521.0
28	5345.0
29	7569.0
30	10803.0
31	15062.0
32	20881.0
33	28717.0
34	42281.0
35	61093.0
36	93558.0
37	159326.0
38	370884.0
39	600516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80627755127237	16.160303158698134	11.881174922344345	28.152244367685146
2	18.555352277478317	19.478311133309575	35.04935728896883	26.916979300243277
3	20.283629037462717	21.10858884171715	29.426442487113512	29.18133963370662
4	15.765500966662083	14.168965236642805	32.67619485887295	37.38933893782217
5	16.613119105676226	35.14062197479664	32.632491631147566	15.61376728837957
6	37.77340823444572	31.955968471944352	14.517328364867938	15.753294928741992
7	30.862054935588596	29.996618787196848	19.80562936856903	19.335696908645524
8	28.575948212727393	34.27785151284721	18.909257227517738	18.236943046907665
9	29.63415558068472	13.661643016883614	16.882142987420767	39.8220584150109
10	17.14555489770779	25.982165154708024	31.980941744930284	24.891338202653902
11	38.3085097129196	20.45654790799734	19.638182170205482	21.596760208877576
12	22.9164152971118	22.770644338502894	28.668686041062795	25.644254323322514
13	32.65416787090221	18.38573044017498	22.5996896579784	26.360412030944406
14	23.863365330923926	18.798490940875073	23.751125901773666	33.587017826427335
15	27.885254825593957	25.566809117208827	20.753561497386226	25.79437455981099
16	25.914681197931426	24.693446059133343	23.392310476708918	25.999562266226313
17	25.94898437346548	23.93365527342928	23.434400262640263	26.68296009046498
18	25.741130980607835	24.964083382671916	24.059924631223325	25.234861005496928
19	27.57933223151627	24.12467275191438	23.766558823281827	24.52943619328752
20	26.03618037998659	23.295363669780375	23.8250636257264	26.843392324506638
21	25.679469444218405	24.649392083191866	24.756861336603244	24.914277135986488
22	26.959700432963597	23.07642663329414	22.891301724839426	27.07257120890283
23	25.78855213942382	23.766979721141137	24.131898165165932	26.312569974269113
24	25.182038324153083	23.273547130739296	24.396713068317336	27.14770147679029
25	26.306607254595505	22.81161173014274	23.548814330730313	27.33296668453144
26	25.662282781629774	23.48553935254685	24.492397181667936	26.359780684155442
27	27.051877064153253	23.888198304623423	23.536397843880565	25.52352678734276
28	25.698900895390047	25.15783669724256	22.403200507322218	26.740061900045177
29	25.914751347574644	24.69295501163081	24.023587116035927	25.368706524758615
30	27.627384737121226	23.429980835117473	22.92279891464472	26.01983551311658
31	26.202996231561166	23.75449308464817	24.167884932137234	25.87462575165343
32	25.687466503545366	23.93183138270559	23.70286294723905	26.677839166509997
33	25.646428962262295	23.236367819833266	23.83151739290254	27.28568582500189
34	26.32154912860113	23.665192588830493	24.941705646485083	25.071552636083293
35	26.16209898956454	23.288278555815264	23.996930251612742	26.552692203007457
36	25.565756872560545	23.143419542568207	25.012206037920095	26.278617546951153
37	24.7711718638199	23.031811460206914	25.72780254839624	26.469214127576947
38	26.044247588956765	22.123022832305875	24.898914364121545	26.93381521461582
39	24.18563279187162	22.957382688751647	26.533330901479037	26.3236536178977
40	26.155995970604494	21.597110957093673	26.299662439916833	25.947230632385004
41	24.735255246491818	22.523717594372314	26.308290846032754	26.43273631310311
42	24.143402706653834	22.37984067613032	27.08940712327537	26.387349493940476
43	23.572033862635774	21.93649493098678	26.498396379156013	27.99307482722143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	27.5
2	34.0
3	79.5
4	125.0
5	125.0
6	159.5
7	194.0
8	183.5
9	173.0
10	260.5
11	348.0
12	348.0
13	581.5
14	815.0
15	1237.5
16	1660.0
17	1600.5
18	1541.0
19	1541.0
20	1854.5
21	2168.0
22	2420.5
23	2673.0
24	3494.5
25	4316.0
26	4316.0
27	5586.5
28	6857.0
29	8524.5
30	10192.0
31	13410.5
32	16629.0
33	16629.0
34	20756.5
35	24884.0
36	29469.0
37	34054.0
38	40741.5
39	47429.0
40	47429.0
41	54115.0
42	60801.0
43	69919.0
44	79037.0
45	92579.5
46	106122.0
47	106122.0
48	120029.0
49	133936.0
50	145777.5
51	157619.0
52	158722.0
53	159825.0
54	159825.0
55	148266.5
56	136708.0
57	126566.5
58	116425.0
59	109056.0
60	101687.0
61	101687.0
62	89187.5
63	76688.0
64	63997.5
65	51307.0
66	43004.5
67	34702.0
68	34702.0
69	28493.0
70	22284.0
71	18176.5
72	14069.0
73	11344.5
74	8620.0
75	8620.0
76	7009.0
77	5398.0
78	4237.0
79	3076.0
80	2409.0
81	1742.0
82	1742.0
83	1265.0
84	788.0
85	579.0
86	370.0
87	254.5
88	139.0
89	139.0
90	93.5
91	48.0
92	31.0
93	14.0
94	9.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1425524.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.45489833699925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.85455381694646	54.88691295182663
2	8.881468065649608	11.626711786408011
3	2.7814583587163813	5.4618022229513254
4	1.3623888221381286	3.5670008739406143
5	0.8136006811334427	2.662707493525142
6	0.5244765155360195	2.0597734202732254
7	0.3677120771927025	1.684798964095461
8	0.260912748500484	1.3662413942340987
9	0.19379268812002165	1.1416212629414837
>10	0.8952280725183784	9.713046734145673
>50	0.03793083850431654	1.6911144900971262
>100	0.023904678724948097	2.941915904520906
>500	0.0022510567792834303	0.94355453838415
>1k	3.215795398976329E-4	0.25279796265601523
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.014964321891458E-5	0.0
3	0.0	0.0	0.0	1.4029928643782916E-4	0.0
4	0.0	0.0	0.0	1.4029928643782916E-4	0.0
5	0.0	0.0	0.0	1.4029928643782916E-4	0.0
6	0.0	0.0	0.0	1.4029928643782916E-4	0.0
7	0.0	0.0	0.0	1.4029928643782916E-4	0.0
8	0.0	0.0	0.0	1.4029928643782916E-4	7.014964321891458E-5
9	0.0	0.0	0.0	1.4029928643782916E-4	7.014964321891458E-5
10	0.0	0.0	0.0	1.4029928643782916E-4	7.014964321891458E-5
11	0.0	0.0	0.0	1.4029928643782916E-4	7.014964321891458E-5
12	0.0	0.0	0.0	1.4029928643782916E-4	7.014964321891458E-5
13	0.0	0.0	0.0	1.4029928643782916E-4	7.014964321891458E-5
14	0.0	0.0	0.0	1.4029928643782916E-4	1.4029928643782916E-4
15	0.0	0.0	0.0	2.1044892965674377E-4	1.4029928643782916E-4
16	0.0	0.0	0.0	2.1044892965674377E-4	2.8059857287565833E-4
17	0.0	0.0	0.0	3.5074821609457296E-4	2.8059857287565833E-4
18	0.0	0.0	0.0	4.2089785931348755E-4	2.8059857287565833E-4
19	0.0	0.0	0.0	6.313467889702313E-4	2.8059857287565833E-4
20	0.0	0.0	0.0	9.820950050648041E-4	2.8059857287565833E-4
21	0.0	0.0	0.0	0.0014029928643782919	2.8059857287565833E-4
22	0.0	0.0	0.0	0.0019641900101296083	2.8059857287565833E-4
23	0.0	0.0	0.0	0.002174638939786352	3.5074821609457296E-4
24	0.0	0.0	0.0	0.003437332517726815	3.5074821609457296E-4
25	0.0	0.0	0.0	0.004489577166010533	3.5074821609457296E-4
26	0.0	0.0	0.0	0.007155263608329288	3.5074821609457296E-4
27	0.0	0.0	0.0	0.017467261161509734	3.5074821609457296E-4
28	0.0	0.0	0.0	0.053313728846375086	3.5074821609457296E-4
29	0.0	0.0	0.0	0.1086617973460987	3.5074821609457296E-4
30	0.0	0.0	0.0	0.18435326237930755	3.5074821609457296E-4
31	0.0	0.0	0.0	0.5246491816342622	3.5074821609457296E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTAC	520	0.0	23.125	31
GTACTTA	590	0.0	22.262712	30
CTTATAC	970	0.0	20.979382	37
ACTTACA	485	0.0	19.835052	32
ATAGTCT	75	9.268331E-6	19.733334	4
AGTGTAC	740	0.0	19.0	27
GTTAACC	60	9.2383014E-4	18.5	3
GTGTACT	760	0.0	18.256578	28
TACATAT	310	0.0	17.903227	35
TTACATA	315	0.0	17.619047	34
TATAAGT	95	3.607618E-6	17.526316	4
ACATATC	140	1.869921E-9	17.178572	36
CATATAA	220	0.0	16.818182	37
ACATATA	220	0.0	16.818182	36
TAATACT	100	5.879785E-6	16.65	4
TGTACTT	845	0.0	16.639053	29
GTATAAT	90	4.447162E-5	16.444445	1
CAGTGTA	855	0.0	16.22807	26
CTTACAT	390	0.0	16.128204	33
TAGAGTG	150	4.6729838E-9	16.033333	5
>>END_MODULE