FastQCFastQC Report
Thu 9 Feb 2017
SRR2727513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183556
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG12140.10257224837692513No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC11860.10020649635505206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTA400.001931220423.1250022
GCTTATA2250.023.0222231
CTTATAC9600.021.77604337
GTAGTAC705.1021325E-621.1428593
ACATATA1850.020.036
CGACTTA2250.018.91111219
TACTTAC5600.018.83035931
GTACTTA5550.018.66666630
CATATAA1800.018.537
TTATAGC2800.018.53
TTACATA2400.018.534
ATAGCAA3200.017.343755
TACATAT2350.017.31914735
AGTGTAC7050.017.31914727
TAGCAAT3000.017.2666666
CTTATAG3000.017.2666662
CTAATAC752.0673311E-417.2666663
ACTTACA4350.016.58620832
TATAGCA2950.016.3050844
TTACACG803.3826704E-416.18754
Produced by FastQC (version 0.11.5)