##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727513.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1183556
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.930013450990067	33.0	31.0	34.0	30.0	34.0
2	32.12203647313689	33.0	31.0	34.0	30.0	34.0
3	32.21067275228211	34.0	31.0	34.0	30.0	34.0
4	35.85556323486172	37.0	35.0	37.0	35.0	37.0
5	35.536182487351674	37.0	35.0	37.0	33.0	37.0
6	35.53575665198774	37.0	35.0	37.0	33.0	37.0
7	35.507400579271284	37.0	35.0	37.0	33.0	37.0
8	35.47618617116554	37.0	35.0	37.0	33.0	37.0
9	37.13170732943773	39.0	37.0	39.0	33.0	39.0
10	36.993239863597495	39.0	37.0	39.0	33.0	39.0
11	37.1124272953709	39.0	37.0	39.0	33.0	39.0
12	37.06403414794061	39.0	37.0	39.0	33.0	39.0
13	37.129284968349616	39.0	37.0	39.0	33.0	39.0
14	38.27940714254332	40.0	38.0	41.0	34.0	41.0
15	38.26444122627066	40.0	38.0	41.0	34.0	41.0
16	38.22188979651153	40.0	38.0	41.0	34.0	41.0
17	38.249150019095	40.0	38.0	41.0	34.0	41.0
18	38.208680451115114	40.0	38.0	41.0	33.0	41.0
19	38.27215104312766	40.0	38.0	41.0	34.0	41.0
20	38.2371463623183	40.0	38.0	41.0	34.0	41.0
21	38.20519350161716	40.0	38.0	41.0	34.0	41.0
22	38.191036165589125	40.0	38.0	41.0	34.0	41.0
23	38.12832345913501	40.0	38.0	41.0	34.0	41.0
24	38.06455714811973	40.0	37.0	41.0	33.0	41.0
25	37.97700320052452	40.0	37.0	41.0	33.0	41.0
26	37.84126902318099	40.0	37.0	41.0	33.0	41.0
27	37.76352280753932	39.0	37.0	41.0	33.0	41.0
28	37.64975801736462	39.0	37.0	40.0	33.0	41.0
29	37.54089709316669	39.0	37.0	40.0	32.0	41.0
30	37.476673685064334	39.0	37.0	40.0	32.0	41.0
31	37.37253328106148	39.0	37.0	40.0	32.0	41.0
32	37.26266522243139	39.0	36.0	40.0	31.0	41.0
33	37.211379943154355	39.0	36.0	40.0	31.0	41.0
34	37.154663573164264	39.0	36.0	40.0	31.0	41.0
35	37.15337423831234	39.0	36.0	40.0	31.0	41.0
36	37.05501471835722	39.0	36.0	40.0	31.0	41.0
37	37.05462774891936	39.0	36.0	40.0	31.0	41.0
38	37.03444450452704	39.0	36.0	40.0	31.0	41.0
39	36.957488281078376	39.0	36.0	40.0	31.0	41.0
40	36.9089954340986	39.0	36.0	40.0	31.0	41.0
41	36.84668068093102	39.0	36.0	40.0	31.0	41.0
42	36.813272037824994	39.0	36.0	40.0	31.0	41.0
43	35.655004072473126	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	0.0
14	3.0
15	3.0
16	8.0
17	19.0
18	35.0
19	73.0
20	158.0
21	303.0
22	602.0
23	1099.0
24	1763.0
25	2845.0
26	4357.0
27	6464.0
28	9185.0
29	12945.0
30	17696.0
31	24112.0
32	31126.0
33	42075.0
34	58103.0
35	82250.0
36	125476.0
37	207736.0
38	351867.0
39	203250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14776825093194	16.544886764969295	12.48635468030241	27.820990303796357
2	18.93556367421567	19.851532162398737	34.929145726944896	26.283758436440692
3	20.568946463031747	21.338660781576877	29.707424067809214	28.38496868758217
4	16.475857500616787	14.63428853387588	32.17194623659548	36.717907728911854
5	16.801063912480693	35.10877389831998	32.42575763208501	15.664404557114322
6	37.836485979539624	31.726677909621515	14.407598795494255	16.029237315344606
7	30.650936668818375	30.27647192021332	19.619350499680625	19.45324091128768
8	28.38192700641119	34.76244470054649	18.835864124722445	18.019764168319877
9	30.03322191767859	13.699985467523293	16.656415074571886	39.61037754022623
10	17.30480011085238	26.23914711259966	31.738675652018156	24.717377124529808
11	38.43899232482451	20.606460530807162	19.182869251645045	21.771677892723286
12	22.71561294945064	22.963847929460034	28.585128206861356	25.73541091422797
13	32.9420830108588	18.424814710922004	22.305999885092046	26.32710239312715
14	23.762120254554915	19.096688285133954	24.022690941535508	33.118500518775626
15	28.328613094775406	25.51767723707201	20.724325676182623	25.42938399196996
16	25.630979860691	24.861687997864063	23.730943022552374	25.776389118892556
17	26.009838148765247	23.924427741484138	23.45043242567314	26.615301684077476
18	25.743606555160888	25.047484022724735	24.128558344514328	25.080351077600042
19	27.74063922619631	24.219724288500082	23.95467557090666	24.08496091439695
20	26.15575435382863	23.095907586966735	23.928060860660587	26.82027719854405
21	25.559584844316618	24.82763806697782	25.005491924336493	24.60728516436907
22	27.110504276941693	23.097005971834033	22.68528062888448	27.1072091223398
23	25.802750355707715	23.91226101680022	24.21465481988178	26.070333807610286
24	25.129862887772102	23.0552673468767	24.659923146855746	27.15494661849545
25	26.269056977447626	22.56124763002342	23.535852971891487	27.633842420637468
26	25.580031701077093	23.38841592624261	24.6935506220238	26.3380017506565
27	27.25963114546333	23.776568240117072	23.570240867352286	25.39355974706731
28	25.688771802939613	25.50162391978073	22.198611641527734	26.610992635751916
29	25.952215188803912	24.814710922001158	24.23729844637685	24.995775442818083
30	27.959640270506846	23.34608586327981	22.823170175302224	25.871103690911117
31	26.13623689964818	23.914711259965728	24.368344210159893	25.5807076302262
32	25.667226561311846	23.818475847361682	23.78763657993369	26.72666101139279
33	25.60174592499214	23.17220308967214	23.892828053763402	27.333222931572315
34	26.265508349414812	23.756036892212958	25.247305577429373	24.731149180942854
35	26.244808019223427	22.857304597332107	24.279290544765097	26.61859683867937
36	25.449915339874075	22.901408974311313	25.44552180040488	26.20315388540973
37	24.329224810655347	22.889580214201946	26.21641899496095	26.56477598018176
38	26.08309197029967	21.691326815123237	25.285242100922982	26.940339113654105
39	23.777244169266176	22.90605598721142	27.08321363754651	26.233486205975893
40	26.25562288560913	21.16055345078729	26.798985430347187	25.78483823325639
41	24.663133810314005	22.182980779954644	26.892010179492985	26.261875230238367
42	23.816279077627083	22.17115201984528	27.740047788190843	26.272521114336794
43	23.341016394661512	21.596358769673763	26.80194262037453	28.260682215290196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	23.0
2	33.0
3	77.0
4	121.0
5	121.0
6	166.5
7	212.0
8	191.0
9	170.0
10	261.5
11	353.0
12	353.0
13	633.0
14	913.0
15	1345.5
16	1778.0
17	1639.0
18	1500.0
19	1500.0
20	1639.0
21	1778.0
22	1854.5
23	1931.0
24	2525.5
25	3120.0
26	3120.0
27	4106.0
28	5092.0
29	6294.5
30	7497.0
31	10157.5
32	12818.0
33	12818.0
34	16583.5
35	20349.0
36	24043.0
37	27737.0
38	32686.0
39	37635.0
40	37635.0
41	43378.5
42	49122.0
43	57184.5
44	65247.0
45	77100.0
46	88953.0
47	88953.0
48	102415.5
49	115878.0
50	126928.0
51	137978.0
52	138970.5
53	139963.0
54	139963.0
55	128410.0
56	116857.0
57	105825.0
58	94793.0
59	88892.5
60	82992.0
61	82992.0
62	71607.5
63	60223.0
64	50017.0
65	39811.0
66	33021.0
67	26231.0
68	26231.0
69	21396.5
70	16562.0
71	13594.5
72	10627.0
73	8583.5
74	6540.0
75	6540.0
76	5369.0
77	4198.0
78	3271.5
79	2345.0
80	1803.0
81	1261.0
82	1261.0
83	908.0
84	555.0
85	391.5
86	228.0
87	160.0
88	92.0
89	92.0
90	65.5
91	39.0
92	22.5
93	6.0
94	5.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1183556.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.41883743681055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.10138701588953	58.909568013240566
2	7.780102612339386	10.64611151750707
3	2.4582461727903624	5.045710358274166
4	1.1933814485887586	3.2659908532439905
5	0.6572053406915557	2.248261268368962
6	0.4121433885502153	1.691902290112406
7	0.29247380982249194	1.4007502634138802
8	0.2069910468802891	1.1329669429902185
9	0.15633466103346452	0.9626612183088742
>10	0.6715470501660888	7.761299719998845
>50	0.039515307000641105	1.8843819631421062
>100	0.028074915729403477	3.7668129232650274
>500	0.0021025199430476068	0.9016133778737565
>1k	4.947105748347309E-4	0.3819692902601724
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1214	0.10257224837692513	No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC	1186	0.10020649635505206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.449114363832382E-5	0.0
3	0.0	0.0	0.0	8.449114363832382E-5	0.0
4	0.0	0.0	0.0	1.6898228727664765E-4	0.0
5	8.449114363832382E-5	0.0	0.0	2.534734309149715E-4	0.0
6	8.449114363832382E-5	0.0	0.0	2.534734309149715E-4	0.0
7	8.449114363832382E-5	0.0	0.0	2.534734309149715E-4	0.0
8	8.449114363832382E-5	0.0	0.0	2.534734309149715E-4	0.0
9	8.449114363832382E-5	0.0	0.0	5.06946861829943E-4	1.6898228727664765E-4
10	8.449114363832382E-5	0.0	0.0	5.914380054682668E-4	1.6898228727664765E-4
11	8.449114363832382E-5	0.0	0.0	5.914380054682668E-4	1.6898228727664765E-4
12	8.449114363832382E-5	0.0	0.0	5.914380054682668E-4	1.6898228727664765E-4
13	8.449114363832382E-5	0.0	0.0	5.914380054682668E-4	1.6898228727664765E-4
14	8.449114363832382E-5	0.0	0.0	6.759291491065906E-4	3.379645745532953E-4
15	8.449114363832382E-5	0.0	0.0	6.759291491065906E-4	3.379645745532953E-4
16	8.449114363832382E-5	0.0	0.0	6.759291491065906E-4	3.379645745532953E-4
17	8.449114363832382E-5	0.0	0.0	8.449114363832383E-4	3.379645745532953E-4
18	8.449114363832382E-5	0.0	0.0	0.0012673671545748574	4.2245571819161915E-4
19	8.449114363832382E-5	0.0	0.0	0.001520840585489829	4.2245571819161915E-4
20	8.449114363832382E-5	0.0	0.0	0.0017743140164048004	4.2245571819161915E-4
21	1.6898228727664765E-4	0.0	0.0	0.002534734309149715	4.2245571819161915E-4
22	1.6898228727664765E-4	0.0	0.0	0.0032106634582563056	4.2245571819161915E-4
23	1.6898228727664765E-4	0.0	0.0	0.004140066038277867	5.06946861829943E-4
24	1.6898228727664765E-4	0.0	0.0	0.005407433192852725	5.06946861829943E-4
25	1.6898228727664765E-4	0.0	0.0	0.006505818060150935	5.06946861829943E-4
26	1.6898228727664765E-4	0.0	0.0	0.008787078938385678	5.06946861829943E-4
27	1.6898228727664765E-4	0.0	0.0	0.021460750484134254	5.06946861829943E-4
28	1.6898228727664765E-4	0.0	0.0	0.06750842376702074	5.06946861829943E-4
29	1.6898228727664765E-4	0.0	0.0	0.14507129362700202	5.06946861829943E-4
30	1.6898228727664765E-4	0.0	0.0	0.24020832136375464	5.06946861829943E-4
31	1.6898228727664765E-4	0.0	0.0	0.6694233310464397	5.06946861829943E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGTA	40	0.0019312204	23.125002	2
GCTTATA	225	0.0	23.022223	1
CTTATAC	960	0.0	21.776043	37
GTAGTAC	70	5.1021325E-6	21.142859	3
ACATATA	185	0.0	20.0	36
CGACTTA	225	0.0	18.911112	19
TACTTAC	560	0.0	18.830359	31
GTACTTA	555	0.0	18.666666	30
CATATAA	180	0.0	18.5	37
TTATAGC	280	0.0	18.5	3
TTACATA	240	0.0	18.5	34
ATAGCAA	320	0.0	17.34375	5
TACATAT	235	0.0	17.319147	35
AGTGTAC	705	0.0	17.319147	27
TAGCAAT	300	0.0	17.266666	6
CTTATAG	300	0.0	17.266666	2
CTAATAC	75	2.0673311E-4	17.266666	3
ACTTACA	435	0.0	16.586208	32
TATAGCA	295	0.0	16.305084	4
TTACACG	80	3.3826704E-4	16.1875	4
>>END_MODULE