FastQCFastQC Report
Thu 9 Feb 2017
SRR2727512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168238
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.15957657784800378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25300.11668460750157501No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22940.10580019352119095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11650.022.2317637
GTATACG609.2404167E-418.51
ACCGTGC1900.017.5263168
GGACCGT1851.8189894E-1217.06
TCTTATA21100.016.74644537
TACTTAC9050.016.5580131
CGTTACA2800.016.51785724
GTATAGG1606.311893E-1016.18751
GTGCGAT803.3842959E-416.187511
GTACTTA9850.015.776648530
TCGTTAC8750.015.64571523
TATACCG957.064289E-515.5789485
AGTGTAC11600.015.15086227
TAGAGTG2500.014.7999995
CTATTAG1252.9614912E-614.7999991
CTCGTTA9400.014.76063922
GTGTACT11500.014.63913128
GTCTTAG3300.014.5757591
GACCGTG2550.014.5098047
ACTTACA8700.014.4597732
Produced by FastQC (version 0.11.5)