FastQCFastQC Report
Thu 9 Feb 2017
SRR2727510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930389
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16000.019.42500137
TACTTAC8600.018.28488331
GTACTTA9900.016.25757630
ACTTACA6850.015.66423332
TCTTATA25600.014.8867237
AGTGTAC10950.014.86757927
CGATCGA750.0041057514.810
ACGGATG17150.014.23906728
TCGTTAC11350.014.18061623
TACGGAT16750.014.13731327
CAGTGTA12050.014.12448126
GTGTACT10900.014.08715528
TTACATA4500.013.97777734
TAGACAG2650.013.9622645
CGTTACG9750.013.85128224
CGGATGG18850.013.83819529
ACAGCTA13800.013.80797119
GTTACGG14500.013.65172425
GAATAGA1652.5974623E-713.4545451
ATACTAC1102.457716E-413.4545453
Produced by FastQC (version 0.11.5)