FastQCFastQC Report
Thu 9 Feb 2017
SRR2727509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654070
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.14114171819130616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32670.12309396511772486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27660.10421729645412518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20250.021.46913537
CGTATAC609.2411606E-418.53
CTAGTAC2100.017.6190473
TATACTA1708.54925E-1116.323535
TACTTAC11700.016.28632431
TAGTACT2100.015.8571424
TCTTATA33350.015.47676237
TACGGAT15850.014.94006427
GTACTTA13350.014.82771630
ATACCGT1508.11342E-814.86
TCGTTAC9850.014.64974523
ACGGATG17050.014.10557228
CCGACTA1458.921852E-714.03448313
AGTGTAC14400.014.00347227
CGTTACG7950.013.72955924
GTATACG950.001245825813.63157751
GTGTTAG3400.013.60294151
ATCTCGT12800.013.585937520
GTATTAT2601.8189894E-1213.5192311
TACACTC3150.013.5079375
Produced by FastQC (version 0.11.5)