##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727509.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2654070
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58752745782892	34.0	31.0	34.0	31.0	34.0
2	32.7574031581684	34.0	31.0	34.0	31.0	34.0
3	32.8474983704273	34.0	31.0	34.0	31.0	34.0
4	36.30422935340816	37.0	37.0	37.0	35.0	37.0
5	36.1658143907282	37.0	35.0	37.0	35.0	37.0
6	36.19013552769897	37.0	36.0	37.0	35.0	37.0
7	36.16585885074621	37.0	36.0	37.0	35.0	37.0
8	36.15542468736695	37.0	36.0	37.0	35.0	37.0
9	37.864334022840396	39.0	38.0	39.0	35.0	39.0
10	37.84163982110494	39.0	38.0	39.0	35.0	39.0
11	37.91793886370744	39.0	38.0	39.0	35.0	39.0
12	37.89342556903171	39.0	38.0	39.0	35.0	39.0
13	37.92238448872863	39.0	38.0	39.0	35.0	39.0
14	39.27497541511716	40.0	39.0	41.0	36.0	41.0
15	39.298448043947594	40.0	39.0	41.0	36.0	41.0
16	39.27465854329388	40.0	39.0	41.0	36.0	41.0
17	39.29234647164544	40.0	39.0	41.0	36.0	41.0
18	39.270774320195024	40.0	39.0	41.0	36.0	41.0
19	39.313325948448984	40.0	39.0	41.0	36.0	41.0
20	39.28731269333514	40.0	39.0	41.0	36.0	41.0
21	39.266441352338106	40.0	39.0	41.0	36.0	41.0
22	39.23718063201046	40.0	39.0	41.0	36.0	41.0
23	39.1961534548825	40.0	39.0	41.0	36.0	41.0
24	39.157335714581755	40.0	39.0	41.0	36.0	41.0
25	39.097804504025895	40.0	39.0	41.0	36.0	41.0
26	38.99855731009355	40.0	39.0	41.0	36.0	41.0
27	38.95299068977081	40.0	38.0	41.0	35.0	41.0
28	38.878793701748634	40.0	38.0	41.0	35.0	41.0
29	38.80009231105434	40.0	38.0	41.0	35.0	41.0
30	38.74423244300263	40.0	38.0	41.0	35.0	41.0
31	38.67325918306601	40.0	38.0	41.0	35.0	41.0
32	38.582008763898465	40.0	38.0	41.0	35.0	41.0
33	38.53649338563037	40.0	38.0	41.0	35.0	41.0
34	38.49081410814334	40.0	38.0	41.0	35.0	41.0
35	38.47386052364858	40.0	38.0	41.0	35.0	41.0
36	38.40834341219335	40.0	38.0	41.0	34.0	41.0
37	38.3990279080808	40.0	38.0	41.0	34.0	41.0
38	38.35693519763985	40.0	38.0	41.0	34.0	41.0
39	38.302634444457006	40.0	38.0	41.0	34.0	41.0
40	38.2438669665834	40.0	38.0	41.0	34.0	41.0
41	38.19057221550298	40.0	38.0	41.0	34.0	41.0
42	38.14666757093822	40.0	38.0	41.0	34.0	41.0
43	37.07037644071181	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	3.0
13	2.0
14	1.0
15	1.0
16	9.0
17	14.0
18	27.0
19	71.0
20	113.0
21	268.0
22	505.0
23	1010.0
24	1720.0
25	2750.0
26	4268.0
27	6731.0
28	9955.0
29	14519.0
30	20225.0
31	27484.0
32	37681.0
33	52067.0
34	75418.0
35	109431.0
36	166631.0
37	287920.0
38	678875.0
39	1156364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2481057394869	16.863797865165576	12.360826956334988	27.527269439012535
2	18.274273097544526	19.7375351818151	35.98944262962168	25.9987490910187
3	20.168797356512826	21.627575761001026	29.422471901645398	28.781154980840746
4	15.487873341697844	14.530438157245287	33.20066916094903	36.78101934010783
5	16.20793724355424	35.18256865870154	33.10364835893552	15.505845738808697
6	36.91296763084621	32.61918487455116	14.825155327478177	15.642692167124453
7	30.341475545106196	29.851887855256265	20.46287400106252	19.34376259857502
8	27.429155975539455	34.81185500005652	19.300508275968607	18.45848074843542
9	28.61002158948332	14.269179034464049	17.940107080823037	39.18069229522959
10	17.03738032531169	26.27394906690479	32.59484489858971	24.0938257091938
11	37.309453028744535	20.87107725116519	20.46524771388848	21.354222006201795
12	22.21697242348544	23.409781957521844	29.637161039460153	24.736084579532566
13	31.724897986865457	19.056166566820014	23.356090834077474	25.86284461223706
14	23.21140738563791	19.431966752949243	24.637971116059486	32.71865474535336
15	26.765043876009297	26.268711827495128	21.62169799590817	25.3445463005874
16	25.285429547826542	25.188182677924846	24.107766562298657	25.418621211949947
17	25.180421013763766	24.83724995949617	24.47282852373901	25.509500503001053
18	24.971647319023234	25.105291118923013	25.19545452832819	24.72760703372556
19	26.388979944010522	24.708843399006057	24.892485880176483	24.00969077680694
20	25.337613552016336	23.991793735658817	24.900586646169845	25.770006066155
21	24.895311728778818	24.97345586212873	25.661757225694874	24.469475183397574
22	25.908434969688066	23.807171626972913	24.21590236881469	26.06849103452433
23	25.012640962747778	24.36145241082564	25.182832404571094	25.44307422185549
24	24.79263922956064	24.034143786712484	25.221565369413767	25.95165161431311
25	25.49586107374711	23.728311611977077	24.62188261801686	26.15394469625895
26	24.968520046569985	24.1922405965178	25.25815068931867	25.581088667593544
27	25.880025771739252	24.667284585561045	24.503724468457875	24.948965174241824
28	25.113655630785924	25.270810491057134	23.90189407212319	25.71363980603375
29	25.034155090106893	25.16041400565923	25.127747195816237	24.67768370841764
30	26.327828580255986	24.201057244157088	24.244462278688957	25.226651896897973
31	25.381320010399122	24.22754486505631	25.102088490507036	25.289046634037536
32	24.869012497786418	24.602327745688697	24.873307787661968	25.655351968862917
33	24.949831767813208	23.836447418493105	25.004916976568065	26.208803837125622
34	25.43365472651437	24.170010587512763	25.73858262969703	24.657752056275832
35	25.402419679963227	23.833320146039856	25.134265486592287	25.62999468740463
36	24.865772191389073	23.722057067070573	25.822265426307524	25.58990531523283
37	24.181916829623937	23.500811960498407	26.352658369975167	25.96461283990249
38	25.03784753228061	22.799285625473328	26.182617640077314	25.980249202168743
39	23.796169656414488	23.232695445108835	27.3122411993655	25.658893699111175
40	25.021457610387067	22.50675377815958	27.205499478159958	25.266289133293395
41	23.89092977954613	22.987374108444765	27.360582049456116	25.761114062552988
42	23.502921927454814	23.279792921814423	27.69953316981089	25.517751980919872
43	23.00696665875429	22.879464369816922	27.2007897304894	26.91277924093939
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	189.0
1	271.5
2	354.0
3	723.0
4	1092.0
5	1092.0
6	1346.0
7	1600.0
8	1472.5
9	1345.0
10	1845.0
11	2345.0
12	2345.0
13	3821.0
14	5297.0
15	6862.0
16	8427.0
17	7877.5
18	7328.0
19	7328.0
20	8624.5
21	9921.0
22	9786.0
23	9651.0
24	12167.5
25	14684.0
26	14684.0
27	18231.5
28	21779.0
29	25823.0
30	29867.0
31	36371.0
32	42875.0
33	42875.0
34	51169.5
35	59464.0
36	69435.5
37	79407.0
38	91345.0
39	103283.0
40	103283.0
41	116489.5
42	129696.0
43	145507.0
44	161318.0
45	181889.5
46	202461.0
47	202461.0
48	222309.5
49	242158.0
50	257100.5
51	272043.0
52	272317.5
53	272592.0
54	272592.0
55	255184.0
56	237776.0
57	218763.0
58	199750.0
59	185967.5
60	172185.0
61	172185.0
62	150462.0
63	128739.0
64	107341.5
65	85944.0
66	71502.0
67	57060.0
68	57060.0
69	46920.0
70	36780.0
71	30205.0
72	23630.0
73	18907.5
74	14185.0
75	14185.0
76	11369.0
77	8553.0
78	6828.5
79	5104.0
80	3979.0
81	2854.0
82	2854.0
83	2093.0
84	1332.0
85	994.5
86	657.0
87	441.5
88	226.0
89	226.0
90	148.0
91	70.0
92	51.5
93	33.0
94	21.5
95	10.0
96	10.0
97	7.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2654070.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.82648388684105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.86195373892377	52.059252014106626
2	9.158644123480261	11.508108148982881
3	3.0226548713037937	5.697083327023482
4	1.4629818584297924	3.6765602462152054
5	0.8352251928615386	2.6237131060599577
6	0.5869529069659396	2.2125712411098073
7	0.3973142459259771	1.747329994877656
8	0.2893967900497832	1.454542621357298
9	0.2149609348304894	1.2154715737585224
>10	1.1019451929917716	11.91173118675585
>50	0.04452711324448121	1.9158934865553017
>100	0.02134460554800445	2.583664200461124
>500	0.0014988753174619708	0.6542866704270153
>1k	5.995501269550076E-4	0.7397921823092685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3746	0.14114171819130616	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3267	0.12309396511772486	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2766	0.10421729645412518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.767798136447042E-5	0.0
3	0.0	0.0	0.0	7.535596272894084E-5	0.0
4	0.0	0.0	0.0	7.535596272894084E-5	0.0
5	0.0	0.0	0.0	1.1303394409341125E-4	0.0
6	0.0	0.0	0.0	1.1303394409341125E-4	3.767798136447042E-5
7	0.0	0.0	0.0	1.1303394409341125E-4	3.767798136447042E-5
8	0.0	0.0	0.0	1.1303394409341125E-4	3.767798136447042E-5
9	0.0	0.0	0.0	1.5071192545788168E-4	7.535596272894084E-5
10	0.0	0.0	0.0	1.883899068223521E-4	7.535596272894084E-5
11	0.0	0.0	0.0	2.637458695512929E-4	7.535596272894084E-5
12	0.0	0.0	0.0	2.637458695512929E-4	7.535596272894084E-5
13	0.0	0.0	0.0	3.3910183228023374E-4	1.1303394409341125E-4
14	0.0	0.0	0.0	4.52135776373645E-4	2.260678881868225E-4
15	0.0	0.0	0.0	5.651697204670563E-4	2.260678881868225E-4
16	3.767798136447042E-5	0.0	0.0	6.028477018315267E-4	2.260678881868225E-4
17	3.767798136447042E-5	0.0	0.0	9.0427155274729E-4	2.260678881868225E-4
18	3.767798136447042E-5	0.0	0.0	0.0012433733850275238	3.0142385091576335E-4
19	3.767798136447042E-5	0.0	0.0	0.0014694412732143463	3.0142385091576335E-4
20	3.767798136447042E-5	0.0	0.0	0.0017331871427656391	3.0142385091576335E-4
21	3.767798136447042E-5	0.0	0.0	0.0022983568632326955	3.0142385091576335E-4
22	3.767798136447042E-5	0.0	0.0	0.0033910183228023374	3.0142385091576335E-4
23	3.767798136447042E-5	0.0	0.0	0.004596713726465391	3.3910183228023374E-4
24	3.767798136447042E-5	0.0	0.0	0.0071964944406138496	3.3910183228023374E-4
25	3.767798136447042E-5	0.0	0.0	0.009457173322482075	3.3910183228023374E-4
26	3.767798136447042E-5	0.0	0.0	0.013827819160760643	3.3910183228023374E-4
27	3.767798136447042E-5	0.0	0.0	0.030556842886585507	3.3910183228023374E-4
28	3.767798136447042E-5	0.0	0.0	0.0803671342504154	3.3910183228023374E-4
29	3.767798136447042E-5	0.0	0.0	0.14867731446420027	3.3910183228023374E-4
30	3.767798136447042E-5	0.0	0.0	0.24140282660216197	3.3910183228023374E-4
31	3.767798136447042E-5	0.0	0.0	0.6634715738469595	3.3910183228023374E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2025	0.0	21.469135	37
CGTATAC	60	9.2411606E-4	18.5	3
CTAGTAC	210	0.0	17.619047	3
TATACTA	170	8.54925E-11	16.32353	5
TACTTAC	1170	0.0	16.286324	31
TAGTACT	210	0.0	15.857142	4
TCTTATA	3335	0.0	15.476762	37
TACGGAT	1585	0.0	14.940064	27
GTACTTA	1335	0.0	14.827716	30
ATACCGT	150	8.11342E-8	14.8	6
TCGTTAC	985	0.0	14.649745	23
ACGGATG	1705	0.0	14.105572	28
CCGACTA	145	8.921852E-7	14.034483	13
AGTGTAC	1440	0.0	14.003472	27
CGTTACG	795	0.0	13.729559	24
GTATACG	95	0.0012458258	13.6315775	1
GTGTTAG	340	0.0	13.6029415	1
ATCTCGT	1280	0.0	13.5859375	20
GTATTAT	260	1.8189894E-12	13.519231	1
TACACTC	315	0.0	13.507937	5
>>END_MODULE