FastQCFastQC Report
Thu 9 Feb 2017
SRR2727508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722211
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12050.021.95435737
TCTTATA23350.017.19271937
TAAGACT1452.9831426E-916.5862064
TATACCG803.3837903E-416.18755
GTACTTA5950.016.16806830
ATACTTA1151.2429991E-616.0869566
TTAGTAC700.002593197915.8571423
ATACCGT700.002593197915.8571426
TACTTAC5850.015.81196531
TATAGGG957.062941E-515.5789482
CGACTTA2850.015.57894719
TAAGGTA750.00410551114.84
GTATTAG1356.5710465E-613.7037041
GCTTATA3250.013.6615381
TAACGAA950.001245514513.63157916
TACCGTC950.001245514513.6315797
TACGGAT11100.013.527
GCGACTT3300.013.45454518
GTCTTAA2202.8376235E-1013.4545451
GTGTAAG3050.013.3442621
Produced by FastQC (version 0.11.5)