##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727508.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1722211
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51632000956909	34.0	31.0	34.0	31.0	34.0
2	32.693553809608694	34.0	31.0	34.0	31.0	34.0
3	32.78552686052986	34.0	31.0	34.0	31.0	34.0
4	36.260893700017014	37.0	37.0	37.0	35.0	37.0
5	36.11318125363268	37.0	35.0	37.0	35.0	37.0
6	36.13118369351955	37.0	35.0	37.0	35.0	37.0
7	36.10658275902314	37.0	35.0	37.0	35.0	37.0
8	36.09296189607429	37.0	35.0	37.0	35.0	37.0
9	37.79756313250815	39.0	38.0	39.0	35.0	39.0
10	37.76667957642821	39.0	37.0	39.0	35.0	39.0
11	37.84748210294789	39.0	38.0	39.0	35.0	39.0
12	37.82321329964795	39.0	38.0	39.0	35.0	39.0
13	37.853887822107744	39.0	38.0	39.0	35.0	39.0
14	39.187762707356995	40.0	39.0	41.0	36.0	41.0
15	39.20073498543442	40.0	39.0	41.0	36.0	41.0
16	39.18235976892495	40.0	39.0	41.0	36.0	41.0
17	39.20170873371497	40.0	39.0	41.0	36.0	41.0
18	39.171193309066076	40.0	39.0	41.0	36.0	41.0
19	39.211931639038426	40.0	39.0	41.0	36.0	41.0
20	39.18473926830104	40.0	39.0	41.0	36.0	41.0
21	39.16519230222081	40.0	39.0	41.0	36.0	41.0
22	39.136805536603816	40.0	39.0	41.0	36.0	41.0
23	39.09158111288338	40.0	39.0	41.0	36.0	41.0
24	39.0548004861193	40.0	39.0	41.0	36.0	41.0
25	38.992639113325836	40.0	38.0	41.0	36.0	41.0
26	38.88661900313028	40.0	38.0	41.0	35.0	41.0
27	38.84003063503833	40.0	38.0	41.0	35.0	41.0
28	38.76854984667965	40.0	38.0	41.0	35.0	41.0
29	38.686303826883	40.0	38.0	41.0	35.0	41.0
30	38.63390258220392	40.0	38.0	41.0	35.0	41.0
31	38.56646427179945	40.0	38.0	41.0	35.0	41.0
32	38.480869649537716	40.0	38.0	41.0	34.0	41.0
33	38.43438986279846	40.0	38.0	41.0	34.0	41.0
34	38.387667945449195	40.0	38.0	41.0	34.0	41.0
35	38.38258726718155	40.0	38.0	41.0	34.0	41.0
36	38.31971518007956	40.0	38.0	41.0	34.0	41.0
37	38.30794310337119	40.0	38.0	41.0	34.0	41.0
38	38.27167751222121	40.0	38.0	41.0	34.0	41.0
39	38.22433720374565	40.0	38.0	41.0	34.0	41.0
40	38.17155098881612	40.0	38.0	41.0	34.0	41.0
41	38.12773115489333	40.0	38.0	41.0	34.0	41.0
42	38.08676985572615	40.0	38.0	41.0	33.0	41.0
43	36.99869644311876	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	1.0
12	0.0
13	2.0
14	1.0
15	3.0
16	3.0
17	9.0
18	20.0
19	34.0
20	92.0
21	190.0
22	380.0
23	670.0
24	1203.0
25	1965.0
26	3201.0
27	4797.0
28	6769.0
29	10089.0
30	13940.0
31	19531.0
32	26392.0
33	36638.0
34	51553.0
35	74320.0
36	112671.0
37	194533.0
38	455156.0
39	708044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.145642432895855	16.649527845310477	12.318002846341129	27.88682687545254
2	18.52949493412828	19.401049000383807	35.67750989861288	26.391946166875023
3	20.198454196378957	21.17127343862047	29.355404186827283	29.274868178173293
4	15.74824455307741	14.430055318424978	32.73518750025403	37.086512628243575
5	16.377958333793014	34.91807914361249	33.0039698968361	15.6999926257584
6	37.34838530238165	32.240706858799534	14.71271522478953	15.69819261402929
7	30.69049030577554	29.628076931340004	20.147299024335577	19.534133738548878
8	27.713619295196697	34.540541199655564	19.06961458264986	18.676224922497884
9	28.77945849840699	14.093278930398192	17.614682521479654	39.51258004971516
10	17.239234913724278	26.08182156541794	32.26323603786063	24.41570748299715
11	37.71825868026624	20.68120572914701	20.17911858651466	21.42141700407209
12	22.4927143073642	23.202325382894433	29.21709360815835	25.08786670158302
13	32.134854556149044	18.720528436991753	22.95642055474039	26.188196452118817
14	23.490617584024257	19.131279500595454	24.159815492991278	33.21828742238901
15	27.06846025254745	26.045705201046793	21.140905498803573	25.744929047602184
16	25.518708218679357	25.00309195563145	23.736986931334197	25.741212894354987
17	25.484449930931806	24.53050177939869	24.0014725257242	25.9835757639453
18	25.3934041763756	24.914252667065767	24.65894132600477	25.033401830553863
19	26.917665721563733	24.31711329215758	24.4081009818193	24.357120004459386
20	25.736567702796	23.745812795296278	24.439688284420434	26.077931217487286
21	25.302764876080808	24.75858068494511	25.17606727630935	24.762587162664737
22	26.37626864536343	23.588573061024462	23.683799487983762	26.351358805628344
23	25.464823996595076	24.089673100450526	24.683386646583955	25.762116256370444
24	25.098434512379725	23.686818862497102	24.788542170500595	26.426204454622575
25	25.916162421445456	23.345919866961715	24.163763905816417	26.57415380577641
26	25.36814594727359	23.894400860289476	24.833019879677927	25.904433312759007
27	26.25810658508162	24.32048105603785	24.099892521880303	25.321519837000228
28	25.51359850796447	25.02504048574768	23.341216610508237	26.12014439577961
29	25.458146533729025	24.834645696723573	24.702780321342736	25.004427448204662
30	26.7669292554745	23.903458983829506	23.687457576336467	25.642154184359523
31	25.686399633958906	23.94503344828247	24.679670493336765	25.688896424421863
32	25.21851271417962	24.258641943408794	24.41652039151997	26.10632495089161
33	25.25224841787679	23.538288862398392	24.538398605048975	26.671064114675847
34	25.827671522246693	23.828729464624253	25.38126861342774	24.96233039970131
35	25.62206373086689	23.382152361121836	24.84678125967143	26.149002648339838
36	25.141692858772824	23.269100011554915	25.534443805085438	26.054763324586826
37	24.440907647204668	22.995904682991807	26.2121772535421	26.351010416261424
38	25.28075828107009	22.299300143826745	25.881439614542007	26.538501960561163
39	23.967272302871134	22.761032184790363	27.261642156506955	26.01005335583155
40	25.300500345195797	21.892149103681255	27.21507411112808	25.592276439994865
41	24.125499140349238	22.529120996207784	27.23319035820814	26.112189505234838
42	23.52638555902848	22.66859287276646	27.77638744613755	26.02863412206751
43	23.04229853368722	22.272996746623964	27.250087242503966	27.43461747718485
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	77.5
2	106.0
3	214.0
4	322.0
5	322.0
6	433.0
7	544.0
8	486.0
9	428.0
10	599.0
11	770.0
12	770.0
13	1308.0
14	1846.0
15	2584.0
16	3322.0
17	3126.5
18	2931.0
19	2931.0
20	3512.5
21	4094.0
22	4350.5
23	4607.0
24	5950.0
25	7293.0
26	7293.0
27	9334.5
28	11376.0
29	13641.5
30	15907.0
31	20141.0
32	24375.0
33	24375.0
34	29904.0
35	35433.0
36	41723.5
37	48014.0
38	55615.5
39	63217.0
40	63217.0
41	72379.0
42	81541.0
43	92211.5
44	102882.0
45	117102.0
46	131322.0
47	131322.0
48	145413.5
49	159505.0
50	170052.0
51	180599.0
52	180788.0
53	180977.0
54	180977.0
55	170438.0
56	159899.0
57	147562.0
58	135225.0
59	126307.5
60	117390.0
61	117390.0
62	102257.5
63	87125.0
64	73280.0
65	59435.0
66	49369.5
67	39304.0
68	39304.0
69	32077.5
70	24851.0
71	20240.0
72	15629.0
73	12665.5
74	9702.0
75	9702.0
76	7694.5
77	5687.0
78	4496.5
79	3306.0
80	2553.0
81	1800.0
82	1800.0
83	1312.5
84	825.0
85	589.5
86	354.0
87	258.0
88	162.0
89	162.0
90	100.5
91	39.0
92	25.0
93	11.0
94	8.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1722211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.85539087025037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.64947416802818	55.08768817426408
2	8.822962642336615	11.620793068893898
3	2.9325140410407897	5.793655752157161
4	1.3986892678009526	3.6844491374822415
5	0.7990977911639207	2.6312448690326846
6	0.5314468572503511	2.0999184306592817
7	0.3827204193678821	1.7642941968048613
8	0.2755560457874563	1.4517480881594835
9	0.2050715011509908	1.2154557478182746
>10	0.9511129011200022	10.338299040353213
>50	0.03300961055580665	1.4858655592302084
>100	0.01711022296787603	2.1688084153881815
>500	8.818081636599761E-4	0.3784540593495722
>1k	3.527232654639903E-4	0.2793254604068501
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.806489448737698E-5	0.0	0.0	0.0	0.0
7	5.806489448737698E-5	0.0	0.0	0.0	0.0
8	5.806489448737698E-5	0.0	0.0	5.806489448737698E-5	0.0
9	5.806489448737698E-5	0.0	0.0	2.3225957794950793E-4	0.0
10	5.806489448737698E-5	0.0	0.0	2.903244724368849E-4	0.0
11	5.806489448737698E-5	0.0	0.0	2.903244724368849E-4	0.0
12	5.806489448737698E-5	0.0	0.0	3.483893669242619E-4	0.0
13	5.806489448737698E-5	5.806489448737698E-5	0.0	3.483893669242619E-4	0.0
14	5.806489448737698E-5	5.806489448737698E-5	0.0	5.225840503863928E-4	0.0
15	5.806489448737698E-5	5.806489448737698E-5	0.0	5.225840503863928E-4	0.0
16	5.806489448737698E-5	5.806489448737698E-5	0.0	6.387138393611468E-4	5.806489448737698E-5
17	5.806489448737698E-5	5.806489448737698E-5	0.0	6.387138393611468E-4	5.806489448737698E-5
18	1.1612978897475396E-4	5.806489448737698E-5	0.0	8.129085228232777E-4	5.806489448737698E-5
19	1.1612978897475396E-4	5.806489448737698E-5	0.0	0.0011032329952601627	5.806489448737698E-5
20	1.1612978897475396E-4	5.806489448737698E-5	0.0	0.0013935574676970477	5.806489448737698E-5
21	1.1612978897475396E-4	5.806489448737698E-5	0.0	0.0017419468346213094	1.1612978897475396E-4
22	1.1612978897475396E-4	5.806489448737698E-5	5.806489448737698E-5	0.0025548553574445874	1.1612978897475396E-4
23	1.1612978897475396E-4	5.806489448737698E-5	5.806489448737698E-5	0.003019374513343603	2.3225957794950793E-4
24	1.1612978897475396E-4	5.806489448737698E-5	5.806489448737698E-5	0.004819386242452289	2.3225957794950793E-4
25	1.1612978897475396E-4	5.806489448737698E-5	5.806489448737698E-5	0.006329073499124091	2.3225957794950793E-4
26	1.1612978897475396E-4	5.806489448737698E-5	5.806489448737698E-5	0.009987161851828841	2.3225957794950793E-4
27	1.1612978897475396E-4	5.806489448737698E-5	5.806489448737698E-5	0.025606618468933248	2.3225957794950793E-4
28	1.7419468346213096E-4	5.806489448737698E-5	5.806489448737698E-5	0.07496177878320369	2.3225957794950793E-4
29	1.7419468346213096E-4	5.806489448737698E-5	5.806489448737698E-5	0.14667192347511426	2.3225957794950793E-4
30	1.7419468346213096E-4	5.806489448737698E-5	5.806489448737698E-5	0.241666090856463	2.3225957794950793E-4
31	1.7419468346213096E-4	5.806489448737698E-5	5.806489448737698E-5	0.6869657666801571	2.3225957794950793E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1205	0.0	21.954357	37
TCTTATA	2335	0.0	17.192719	37
TAAGACT	145	2.9831426E-9	16.586206	4
TATACCG	80	3.3837903E-4	16.1875	5
GTACTTA	595	0.0	16.168068	30
ATACTTA	115	1.2429991E-6	16.086956	6
TTAGTAC	70	0.0025931979	15.857142	3
ATACCGT	70	0.0025931979	15.857142	6
TACTTAC	585	0.0	15.811965	31
TATAGGG	95	7.062941E-5	15.578948	2
CGACTTA	285	0.0	15.578947	19
TAAGGTA	75	0.004105511	14.8	4
GTATTAG	135	6.5710465E-6	13.703704	1
GCTTATA	325	0.0	13.661538	1
TAACGAA	95	0.0012455145	13.631579	16
TACCGTC	95	0.0012455145	13.631579	7
TACGGAT	1110	0.0	13.5	27
GCGACTT	330	0.0	13.454545	18
GTCTTAA	220	2.8376235E-10	13.454545	1
GTGTAAG	305	0.0	13.344262	1
>>END_MODULE