FastQCFastQC Report
Thu 9 Feb 2017
SRR2727507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4228569
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64560.1526757633610803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58820.13910143124068686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49220.11639871549926227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC32800.020.53048737
TACTTAC20300.016.58620631
AGTGTAC23900.016.02301227
GTACTTA22000.015.89318330
TCTTATA55000.015.00181937
GTGTACT26200.013.91030628
CAGTGTA28300.013.72791526
ACTTACA19600.013.49744932
TACGGAT24650.013.43407827
ACGGATG26150.012.94646428
GTTACGG18450.012.83468825
CTACAGT30500.012.6770523
CGTTACG10100.012.63861324
CGGATGG28650.012.59162229
ACAGCTA32950.012.57663219
TAAGACT5150.012.5728154
TATACTG6200.012.5322585
TTACGGA27450.012.40072826
CAGCTAC34200.012.38742720
CTTACAT15850.012.37223933
Produced by FastQC (version 0.11.5)