FastQCFastQC Report
Thu 9 Feb 2017
SRR2727506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604447
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13400.017.67164237
TCTTATA23400.015.73290637
TACTTAC4900.015.47959131
TTATACC2151.2732926E-1114.6279074
CTCTTAT38050.014.19710937
GTACTTA5250.014.09523830
TACGGAT8300.014.04216927
CGTATAC800.006301443613.8750013
CGTTACG4950.013.82828324
TTTATAC2501.0913936E-1113.323
TATACCG1254.8489863E-513.325
GTTCTAA3350.013.2537321
TCGTTAC6600.013.17424323
TAATACT3550.013.028174
CTCGTTA6900.012.86956522
GTATTAA2602.3646862E-1112.8076931
ATTACTC2358.094503E-1012.5957453
ATTATAC2358.094503E-1012.5957453
TAAGACT3250.012.5230774
TAGACAG5400.012.3333345
Produced by FastQC (version 0.11.5)