##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727506.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2604447
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.606676196520795	34.0	31.0	34.0	31.0	34.0
2	32.78297888188932	34.0	31.0	34.0	31.0	34.0
3	32.878721279411714	34.0	31.0	34.0	31.0	34.0
4	36.330290844851135	37.0	37.0	37.0	35.0	37.0
5	36.19162225224779	37.0	35.0	37.0	35.0	37.0
6	36.20890845542259	37.0	36.0	37.0	35.0	37.0
7	36.18418228514537	37.0	36.0	37.0	35.0	37.0
8	36.17149437097395	37.0	36.0	37.0	35.0	37.0
9	37.89275727246513	39.0	38.0	39.0	35.0	39.0
10	37.86668455914058	39.0	38.0	39.0	35.0	39.0
11	37.94153039013656	39.0	38.0	39.0	35.0	39.0
12	37.914628326089954	39.0	38.0	39.0	35.0	39.0
13	37.94602501029969	39.0	38.0	39.0	35.0	39.0
14	39.31451897466142	40.0	39.0	41.0	36.0	41.0
15	39.331474973382065	40.0	39.0	41.0	36.0	41.0
16	39.32055864450304	40.0	39.0	41.0	36.0	41.0
17	39.330465162086234	40.0	39.0	41.0	36.0	41.0
18	39.30541877028022	40.0	39.0	41.0	36.0	41.0
19	39.33786673332189	40.0	39.0	41.0	36.0	41.0
20	39.31556756578268	40.0	39.0	41.0	36.0	41.0
21	39.296176501192	40.0	39.0	41.0	36.0	41.0
22	39.26470724879408	40.0	39.0	41.0	36.0	41.0
23	39.227519316000674	40.0	39.0	41.0	36.0	41.0
24	39.18689264938008	40.0	39.0	41.0	36.0	41.0
25	39.13228374391954	40.0	39.0	41.0	36.0	41.0
26	39.04308361813467	40.0	39.0	41.0	36.0	41.0
27	38.997186734842366	40.0	39.0	41.0	36.0	41.0
28	38.940833889113506	40.0	38.0	41.0	35.0	41.0
29	38.860153422204405	40.0	38.0	41.0	35.0	41.0
30	38.799882278272506	40.0	38.0	41.0	35.0	41.0
31	38.74303527773842	40.0	38.0	41.0	35.0	41.0
32	38.663377676719854	40.0	38.0	41.0	35.0	41.0
33	38.61783211560842	40.0	38.0	41.0	35.0	41.0
34	38.581048107333345	40.0	38.0	41.0	35.0	41.0
35	38.569580797766285	40.0	38.0	41.0	35.0	41.0
36	38.51893472971422	40.0	38.0	41.0	35.0	41.0
37	38.5007416161665	40.0	38.0	41.0	35.0	41.0
38	38.46209809606415	40.0	38.0	41.0	34.0	41.0
39	38.41818051970341	40.0	38.0	41.0	34.0	41.0
40	38.36154508039519	40.0	38.0	41.0	34.0	41.0
41	38.32356504087048	40.0	38.0	41.0	34.0	41.0
42	38.28461550571004	40.0	38.0	41.0	34.0	41.0
43	37.21839530618208	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	1.0
12	1.0
13	1.0
14	0.0
15	2.0
16	2.0
17	6.0
18	16.0
19	40.0
20	116.0
21	229.0
22	465.0
23	827.0
24	1519.0
25	2621.0
26	3968.0
27	6105.0
28	9098.0
29	12888.0
30	18443.0
31	25796.0
32	35566.0
33	48537.0
34	70220.0
35	103746.0
36	159343.0
37	278084.0
38	665929.0
39	1160872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45816981493576	16.588627067473443	11.924604340191987	28.028598777398813
2	18.34589070155776	19.07253248002359	35.53380045744836	27.047776360970293
3	20.103307919109124	20.93588389397058	28.890854757267086	30.069953429653207
4	15.312156476979567	14.270937362134841	32.98888401261381	37.428022148271786
5	16.38428426456749	34.62020920371964	33.24137523243898	15.754131299273896
6	37.513184180749306	32.54971208859308	14.672212565661733	15.26489116499587
7	31.198945495915254	29.0960422692418	20.159020321780403	19.545991913062544
8	27.919823286862815	34.39359679809188	18.821807470069462	18.864772444975845
9	28.207331537174685	14.109674721735555	17.58634366527712	40.09665007581264
10	17.158882480618725	25.83949682984526	32.37696908403204	24.62465160550397
11	37.9264004988391	20.387475729012724	20.27655006993807	21.409573702210103
12	22.760225107287653	23.117268272305022	29.29328183679683	24.829224783610492
13	32.10489597215839	18.551807735000942	23.04258831145345	26.300707981387216
14	23.576367651175087	18.635357141074476	24.040535284457697	33.747739923292734
15	26.580076307945603	25.87558894460129	21.141340177012623	26.40299457044048
16	26.06918090481396	24.602612377982737	23.230382495785094	26.097824221418215
17	25.588656632290846	24.51268925802675	24.051708481685363	25.846945627997037
18	25.74373753814149	24.42518507767676	24.441695300384303	25.389382083797447
19	26.775549665629594	24.15660598967842	24.183444700544875	24.884399644147106
20	26.002180117314733	23.80198176426704	24.234933557872363	25.960904560545867
21	25.682534526523288	24.250023133509725	24.589788158484318	25.477654181482674
22	26.40253382003934	23.627510945701715	23.696085963738174	26.273869270520766
23	25.786894492381684	23.813846087096415	24.229250969591625	26.170008450930276
24	25.61215490274903	23.769345277519566	24.27056492222725	26.347934897504153
25	26.203528042613268	23.448624602458796	23.917860490153956	26.42998686477398
26	25.778754568628198	23.813154961494707	24.29245056628144	26.115639903595657
27	26.211975133300854	24.106422591820838	23.88096206219593	25.800640212682385
28	25.910874746155326	24.434976023701	23.52902554745787	26.125123682685807
29	25.838844100110308	24.406601478164077	24.232322638932562	25.52223178279305
30	26.411249681794253	23.975147123362465	23.79576163385164	25.817841560991646
31	25.972308132974103	23.742698545986922	24.18732268308781	26.097670637951165
32	25.45373355649011	24.083615446964366	24.250522279777627	26.212128716767896
33	25.574450161589006	23.683492119440327	24.24725863110288	26.494799087867786
34	25.976262907250558	23.715898230987232	24.767177062923533	25.540661798838677
35	25.82778609048293	23.661260912585284	24.463427360971444	26.04752563596034
36	25.507257394755968	23.433842193755527	24.923755407577886	26.13514500391062
37	25.11535078271894	23.095382628250835	25.330367636584654	26.45889895244557
38	25.398021153818835	22.825114122114982	25.34660908822487	26.430255635841316
39	24.68639215925684	22.94479403881131	26.175537455743964	26.193276346187886
40	25.33881472727224	22.461274888680784	26.30316531685997	25.896745067187005
41	24.709045720646262	22.67606136734593	26.400690818434775	26.21420209357303
42	24.209285118875524	22.796202034443397	26.742797991281837	26.251714855399243
43	23.872438179774825	22.4027595877359	26.71046867146845	27.014333561020827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	56.5
2	78.0
3	181.5
4	285.0
5	285.0
6	367.0
7	449.0
8	434.0
9	419.0
10	554.0
11	689.0
12	689.0
13	1185.5
14	1682.0
15	2390.5
16	3099.0
17	3025.5
18	2952.0
19	2952.0
20	3713.0
21	4474.0
22	5198.5
23	5923.0
24	7856.0
25	9789.0
26	9789.0
27	12699.0
28	15609.0
29	19231.5
30	22854.0
31	28792.5
32	34731.0
33	34731.0
34	43224.5
35	51718.0
36	61418.0
37	71118.0
38	83886.5
39	96655.0
40	96655.0
41	110785.5
42	124916.0
43	141719.5
44	158523.0
45	177562.5
46	196602.0
47	196602.0
48	215696.5
49	234791.0
50	247609.0
51	260427.0
52	262107.0
53	263787.0
54	263787.0
55	254553.0
56	245319.0
57	228595.5
58	211872.0
59	197406.0
60	182940.0
61	182940.0
62	160856.5
63	138773.0
64	117312.5
65	95852.0
66	80095.0
67	64338.0
68	64338.0
69	52809.5
70	41281.0
71	33693.0
72	26105.0
73	21001.0
74	15897.0
75	15897.0
76	12696.0
77	9495.0
78	7531.0
79	5567.0
80	4302.0
81	3037.0
82	3037.0
83	2203.5
84	1370.0
85	1008.0
86	646.0
87	450.0
88	254.0
89	254.0
90	159.5
91	65.0
92	42.5
93	20.0
94	13.5
95	7.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2604447.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.61566977054748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9621137054362	52.140747591810936
2	9.7674337292001	12.427236772450012
3	3.1981521581580066	6.103577747080304
4	1.5341623494272403	3.9038706158228234
5	0.875894654774932	2.786031255597488
6	0.5582959449633152	2.130982228141321
7	0.39353805907615935	1.7524631045831809
8	0.2712571175978066	1.3804962572810102
9	0.227897561663275	1.3048070421855495
>10	1.1579724172816062	12.645542631832718
>50	0.04096359325401667	1.7582733303375075
>100	0.01147224634414764	1.2252615550875592
>500	6.650779325145449E-4	0.3015504768274118
>1k	1.8138489068189928E-4	0.1391593909622579
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.679173352346967E-5
2	0.0	0.0	0.0	0.0	7.679173352346967E-5
3	0.0	0.0	0.0	0.0	7.679173352346967E-5
4	0.0	0.0	0.0	0.0	7.679173352346967E-5
5	0.0	0.0	0.0	0.0	7.679173352346967E-5
6	0.0	0.0	0.0	0.0	1.151876002852045E-4
7	0.0	0.0	0.0	0.0	1.151876002852045E-4
8	0.0	0.0	0.0	0.0	1.5358346704693934E-4
9	0.0	0.0	0.0	0.0	3.071669340938787E-4
10	0.0	0.0	0.0	0.0	3.071669340938787E-4
11	0.0	0.0	0.0	0.0	3.071669340938787E-4
12	0.0	0.0	0.0	0.0	3.071669340938787E-4
13	0.0	0.0	0.0	0.0	3.071669340938787E-4
14	0.0	3.8395866761734835E-5	0.0	0.0	3.455628008556135E-4
15	0.0	3.8395866761734835E-5	0.0	0.0	4.991462679025528E-4
16	0.0	3.8395866761734835E-5	0.0	7.679173352346967E-5	5.375421346642877E-4
17	0.0	3.8395866761734835E-5	0.0	2.687710673321438E-4	5.375421346642877E-4
18	0.0	3.8395866761734835E-5	0.0	3.455628008556135E-4	5.759380014260225E-4
19	0.0	3.8395866761734835E-5	0.0	4.2235453437908314E-4	5.759380014260225E-4
20	0.0	3.8395866761734835E-5	0.0	4.60750401140818E-4	5.759380014260225E-4
21	0.0	3.8395866761734835E-5	0.0	9.598966690433708E-4	6.143338681877574E-4
22	0.0	3.8395866761734835E-5	0.0	0.0014974388037076584	6.143338681877574E-4
23	0.0	3.8395866761734835E-5	0.0	0.0019197933380867417	8.447090687581663E-4
24	0.0	3.8395866761734835E-5	0.0	0.0026109189397979684	8.831049355199011E-4
25	0.0	3.8395866761734835E-5	0.0	0.003225252807985726	8.831049355199011E-4
26	0.0	3.8395866761734835E-5	0.0	0.004569108144646445	9.21500802281636E-4
27	0.0	3.8395866761734835E-5	0.0	0.013054594698989843	9.21500802281636E-4
28	0.0	3.8395866761734835E-5	0.0	0.04592145664703486	9.21500802281636E-4
29	0.0	3.8395866761734835E-5	0.0	0.09549052063643453	9.21500802281636E-4
30	0.0	3.8395866761734835E-5	0.0	0.1593812429279613	9.21500802281636E-4
31	0.0	3.8395866761734835E-5	0.0	0.4481181609762072	9.598966690433708E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1340	0.0	17.671642	37
TCTTATA	2340	0.0	15.732906	37
TACTTAC	490	0.0	15.479591	31
TTATACC	215	1.2732926E-11	14.627907	4
CTCTTAT	3805	0.0	14.197109	37
GTACTTA	525	0.0	14.095238	30
TACGGAT	830	0.0	14.042169	27
CGTATAC	80	0.0063014436	13.875001	3
CGTTACG	495	0.0	13.828283	24
TTTATAC	250	1.0913936E-11	13.32	3
TATACCG	125	4.8489863E-5	13.32	5
GTTCTAA	335	0.0	13.253732	1
TCGTTAC	660	0.0	13.174243	23
TAATACT	355	0.0	13.02817	4
CTCGTTA	690	0.0	12.869565	22
GTATTAA	260	2.3646862E-11	12.807693	1
ATTACTC	235	8.094503E-10	12.595745	3
ATTATAC	235	8.094503E-10	12.595745	3
TAAGACT	325	0.0	12.523077	4
TAGACAG	540	0.0	12.333334	5
>>END_MODULE