##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727505.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1456083
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.008934243446284	33.0	31.0	34.0	30.0	34.0
2	32.19823801253088	33.0	31.0	34.0	30.0	34.0
3	32.29062628984749	34.0	31.0	34.0	30.0	34.0
4	35.92756456877802	37.0	35.0	37.0	35.0	37.0
5	35.59835119289216	37.0	35.0	37.0	33.0	37.0
6	35.59709851704882	37.0	35.0	37.0	33.0	37.0
7	35.57461902927237	37.0	35.0	37.0	33.0	37.0
8	35.5353863756393	37.0	35.0	37.0	33.0	37.0
9	37.19517637387429	39.0	37.0	39.0	34.0	39.0
10	37.07454932170762	39.0	37.0	39.0	33.0	39.0
11	37.181017840329154	39.0	37.0	39.0	34.0	39.0
12	37.13632052568432	39.0	37.0	39.0	33.0	39.0
13	37.19080643067737	39.0	37.0	39.0	34.0	39.0
14	38.39131423140027	40.0	38.0	41.0	34.0	41.0
15	38.369325100286176	40.0	38.0	41.0	34.0	41.0
16	38.35510819094791	40.0	38.0	41.0	34.0	41.0
17	38.351675007537345	40.0	38.0	41.0	34.0	41.0
18	38.31606783404518	40.0	38.0	41.0	34.0	41.0
19	38.35497427001071	40.0	38.0	41.0	34.0	41.0
20	38.33270974250781	40.0	38.0	41.0	34.0	41.0
21	38.30201437692769	40.0	38.0	41.0	34.0	41.0
22	38.27144331744825	40.0	38.0	41.0	34.0	41.0
23	38.2194799334928	40.0	38.0	41.0	34.0	41.0
24	38.16145576866154	40.0	38.0	41.0	34.0	41.0
25	38.08729790815496	40.0	38.0	41.0	34.0	41.0
26	37.94971440501675	40.0	37.0	41.0	33.0	41.0
27	37.88778386946348	40.0	37.0	41.0	33.0	41.0
28	37.804092211776386	40.0	37.0	41.0	33.0	41.0
29	37.696664956599314	39.0	37.0	41.0	33.0	41.0
30	37.63335469200588	39.0	37.0	40.0	33.0	41.0
31	37.55469846155748	39.0	37.0	40.0	32.0	41.0
32	37.44719634801038	39.0	37.0	40.0	32.0	41.0
33	37.397002780748075	39.0	37.0	40.0	32.0	41.0
34	37.34283622568219	39.0	37.0	40.0	32.0	41.0
35	37.321219326096106	39.0	37.0	40.0	32.0	41.0
36	37.2675973828415	39.0	36.0	40.0	31.0	41.0
37	37.23360138123994	39.0	36.0	40.0	31.0	41.0
38	37.18682932222957	39.0	36.0	40.0	31.0	41.0
39	37.13203093504972	39.0	36.0	40.0	31.0	41.0
40	37.0750678361055	39.0	36.0	40.0	31.0	41.0
41	37.037153788623314	39.0	36.0	40.0	31.0	41.0
42	36.98351261569567	39.0	36.0	40.0	31.0	41.0
43	35.85575822257385	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	2.0
14	2.0
15	2.0
16	8.0
17	12.0
18	39.0
19	83.0
20	212.0
21	373.0
22	687.0
23	1216.0
24	2107.0
25	3271.0
26	4907.0
27	7366.0
28	10496.0
29	14932.0
30	20166.0
31	27358.0
32	36136.0
33	48260.0
34	66588.0
35	96136.0
36	147584.0
37	250901.0
38	438575.0
39	278661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.939447819938835	17.1502586047636	12.21983911631411	27.69045445898345
2	18.82372090052559	19.66694206305547	34.91964400381023	26.589693032608718
3	20.48612613429317	21.327424329519676	28.815115621842985	29.371333914344167
4	15.760708695864178	14.631171437342513	32.70232534821161	36.9057945185817
5	16.510253879758228	34.739228464311445	32.99550918457258	15.755008471357746
6	37.66536660341478	32.633991331538105	14.45082457524743	15.249817489799689
7	31.15674037812405	29.159738833569236	20.015136499773707	19.668384288533
8	27.923545567113962	34.35223129450725	18.75676043192593	18.96746270645286
9	28.281972936982303	14.084224594339744	17.542475257248384	40.091327211429565
10	17.163994085501994	25.929085086495757	32.35454297591552	24.552377852086728
11	38.02901345596371	20.387917447013663	20.12872892548021	21.45434017154242
12	22.78633841614798	23.222302574784543	29.10898623224088	24.882372776826596
13	32.20551300990397	18.559724960733696	22.960229602296025	26.274532427066315
14	23.63319948107354	18.77502862130799	24.031597099890597	33.560174797727875
15	26.78727792303049	25.88650509620674	21.030188526340876	26.2960284544219
16	26.072964247230413	24.710679267596696	23.169489651345422	26.046866833827465
17	25.66543253372232	24.530263728097918	24.04327225851823	25.76103147966153
18	25.854982167912134	24.374503376524554	24.42456920381599	25.345945251747327
19	26.84345603925051	24.196903610577145	24.130698593418096	24.82894175675425
20	26.07117863473442	23.889160164633473	24.08434134592602	25.955319854706083
21	25.757185545054778	24.32890158047309	24.486859608964597	25.42705326550753
22	26.50638734193037	23.72543323423184	23.590344781169755	26.177834642668035
23	25.943919405693222	23.826869759484865	24.016556748482056	26.21265408633986
24	25.685280303389295	23.87398245841755	24.111400242980654	26.3293369952125
25	26.303033549598474	23.481971838143842	23.792531057638886	26.422463554618798
26	25.822909820387984	23.83092172630269	24.24717547007966	26.098992983229664
27	26.376655726356258	24.151988588562602	23.790402058124435	25.680953626956704
28	26.01383300265163	24.57304975059801	23.358627221113082	26.05449002563727
29	25.847633685717092	24.509179765164486	24.12094640209384	25.52224014702459
30	26.529943691396713	24.0375720340118	23.650162799785452	25.782321474806036
31	26.09397953276015	23.772065191338683	24.10219747088593	26.03175780501524
32	25.543186755150632	24.173278583707113	23.97315262934874	26.310382031793516
33	25.691323914914193	23.660670442550323	24.14168697800881	26.506318664526678
34	26.122205945677546	23.75166800244217	24.637469155261066	25.488656896619215
35	25.974000108510296	23.600783746530933	24.385079696693115	26.040136448265656
36	25.751485320548344	23.441726879580354	24.772626285726844	26.034161514144454
37	25.19684660833208	23.108366762059582	25.17672412905034	26.518062500558003
38	25.595038194938063	22.78805535124028	25.17081787233283	26.44608858148883
39	24.735815197347954	22.99298872385709	26.066645926090754	26.204550152704208
40	25.610147223750296	22.38375147570571	26.246237336745224	25.759863963798768
41	24.886493421048115	22.62061984103928	26.280438683783824	26.212448054128785
42	24.245801922005818	22.852749465518105	26.627122217620837	26.27432639485524
43	23.985651916820675	22.475298454827094	26.469301543936712	27.06974808441552
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	34.5
2	45.0
3	96.5
4	148.0
5	148.0
6	204.5
7	261.0
8	254.5
9	248.0
10	323.5
11	399.0
12	399.0
13	707.0
14	1015.0
15	1424.0
16	1833.0
17	1768.5
18	1704.0
19	1704.0
20	2098.5
21	2493.0
22	2879.5
23	3266.0
24	4286.0
25	5306.0
26	5306.0
27	6889.5
28	8473.0
29	10393.0
30	12313.0
31	15623.5
32	18934.0
33	18934.0
34	23467.0
35	28000.0
36	33369.5
37	38739.0
38	45858.0
39	52977.0
40	52977.0
41	60981.0
42	68985.0
43	78324.5
44	87664.0
45	99076.5
46	110489.0
47	110489.0
48	121243.0
49	131997.0
50	139619.0
51	147241.0
52	148564.0
53	149887.0
54	149887.0
55	144090.0
56	138293.0
57	128615.5
58	118938.0
59	111044.5
60	103151.0
61	103151.0
62	90260.5
63	77370.0
64	65358.0
65	53346.0
66	44402.0
67	35458.0
68	35458.0
69	29127.0
70	22796.0
71	18445.5
72	14095.0
73	11420.5
74	8746.0
75	8746.0
76	6985.0
77	5224.0
78	4158.5
79	3093.0
80	2404.5
81	1716.0
82	1716.0
83	1265.0
84	814.0
85	614.5
86	415.0
87	270.5
88	126.0
89	126.0
90	85.5
91	45.0
92	27.5
93	10.0
94	7.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1456083.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.82253865307649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.57692543326618	59.05375645222597
2	8.686162132917866	12.129797825450943
3	2.7236867934832105	5.705241792505664
4	1.2452121389300757	3.4777549080690107
5	0.7355297317539584	2.567827656293887
6	0.4792594342304683	2.0077866222845064
7	0.3260914607458805	1.5937973535657193
8	0.23908680262947815	1.3354918014429766
9	0.1709733187868432	1.0744012043675215
>10	0.7882929356871522	8.86433743251336
>50	0.020417195251268814	0.9767864186154354
>100	0.007673992863630631	0.9032359989524625
>500	6.886294540605468E-4	0.30978453371255804
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3735480738391974E-4
2	0.0	0.0	0.0	0.0	1.3735480738391974E-4
3	0.0	0.0	0.0	1.3735480738391974E-4	2.060322110758796E-4
4	0.0	0.0	0.0	1.3735480738391974E-4	2.747096147678395E-4
5	0.0	0.0	0.0	1.3735480738391974E-4	2.747096147678395E-4
6	0.0	0.0	0.0	1.3735480738391974E-4	2.747096147678395E-4
7	0.0	0.0	0.0	2.060322110758796E-4	2.747096147678395E-4
8	0.0	0.0	0.0	2.060322110758796E-4	2.747096147678395E-4
9	0.0	0.0	0.0	2.747096147678395E-4	2.747096147678395E-4
10	0.0	0.0	0.0	2.747096147678395E-4	2.747096147678395E-4
11	0.0	0.0	0.0	2.747096147678395E-4	2.747096147678395E-4
12	0.0	0.0	0.0	2.747096147678395E-4	2.747096147678395E-4
13	0.0	0.0	0.0	2.747096147678395E-4	2.747096147678395E-4
14	0.0	0.0	0.0	2.747096147678395E-4	4.120644221517592E-4
15	0.0	0.0	0.0	4.120644221517592E-4	6.180966332276388E-4
16	0.0	0.0	0.0	5.49419229535679E-4	7.554514406115585E-4
17	0.0	0.0	0.0	5.49419229535679E-4	7.554514406115585E-4
18	6.867740369195987E-5	0.0	0.0	6.180966332276388E-4	0.001030161055379398
19	6.867740369195987E-5	0.0	0.0	7.554514406115585E-4	0.001030161055379398
20	6.867740369195987E-5	0.0	0.0	9.614836516874381E-4	0.001030161055379398
21	6.867740369195987E-5	0.0	0.0	0.001510902881223117	0.001030161055379398
22	6.867740369195987E-5	0.0	0.0	0.001991644707066836	0.001030161055379398
23	6.867740369195987E-5	0.0	0.0	0.0025410639366025153	0.001098838459071358
24	6.867740369195987E-5	0.0	0.0	0.003159160569830154	0.001098838459071358
25	6.867740369195987E-5	0.0	0.0	0.003983289414133672	0.001098838459071358
26	6.867740369195987E-5	0.0	0.0	0.005768901910124629	0.001098838459071358
27	6.867740369195987E-5	0.0	0.0	0.012499287471936696	0.001098838459071358
28	6.867740369195987E-5	0.0	0.0	0.045395763840385475	0.001098838459071358
29	6.867740369195987E-5	0.0	0.0	0.0929205271952217	0.001098838459071358
30	6.867740369195987E-5	0.0	0.0	0.15837009291365944	0.001098838459071358
31	6.867740369195987E-5	0.0	0.0	0.4438620600611366	0.001098838459071358
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	55	5.143999E-4	20.181818	1
CTTATAC	725	0.0	20.15862	37
CGCAATA	75	2.0677436E-4	17.266666	36
TACTTAC	295	0.0	16.932203	31
TCTTATA	1195	0.0	16.410042	37
TATACTC	80	3.3833404E-4	16.1875	5
TAGACTG	245	0.0	15.857142	5
TCTAGTA	105	9.346442E-6	15.857142	2
CCGTATC	75	0.004105105	14.8	9
ATACGAC	75	0.004105105	14.8	3
GTACTTA	375	0.0	14.306666	30
CTCTTAT	2000	0.0	14.1525	37
TACGACC	105	1.6561095E-4	14.095238	4
GTTCTAG	165	2.595989E-7	13.454545	1
TATACTG	195	1.0260919E-8	13.28205	5
GTAAAAC	195	1.0260919E-8	13.28205	3
CTAGATA	155	1.8883002E-6	13.129032	4
TTGTACG	85	0.009406307	13.058823	7
CGTTATG	85	0.009406307	13.058823	24
CTCGTTA	370	0.0	13.000001	22
>>END_MODULE