FastQCFastQC Report
Thu 9 Feb 2017
SRR2727504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038976
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTA10610.10211977947517557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT250.00549610329.62
CTTATAC4850.020.97938237
GATATAT555.142636E-420.1818181
ATACCGA555.142636E-420.1818186
TCTTATA10450.018.94258537
TATACCG500.007034437718.55
TACTTAC4400.017.6590931
GGTATCA8000.017.5751
GTCTAGA953.6057136E-617.5263161
GCTCTAT752.0670252E-417.2666661
GTGTAGA1757.2759576E-1216.9142861
TAAGATT1107.805811E-716.8181824
TCGTTAC5350.016.25233723
AGGGCGT5600.016.187510
GTACTTA5100.015.96078430
GGGCGTC5350.015.56074711
GGCGTCA6100.015.46721312
GTCCTAT855.363533E-415.2352941
ACTGTCG855.363533E-415.2352948
CGTTACG4500.015.21111124
Produced by FastQC (version 0.11.5)