##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727504.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038976
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08424737433781	33.0	31.0	34.0	30.0	34.0
2	32.27517767494148	34.0	31.0	34.0	30.0	34.0
3	32.3607994794875	34.0	31.0	34.0	30.0	34.0
4	35.96729953338672	37.0	35.0	37.0	35.0	37.0
5	35.702363673463104	37.0	35.0	37.0	33.0	37.0
6	35.70483245041272	37.0	35.0	37.0	33.0	37.0
7	35.682360324011334	37.0	35.0	37.0	33.0	37.0
8	35.65867257761488	37.0	35.0	37.0	33.0	37.0
9	37.322838063631885	39.0	37.0	39.0	34.0	39.0
10	37.21206649624246	39.0	37.0	39.0	34.0	39.0
11	37.31944048755698	39.0	37.0	39.0	34.0	39.0
12	37.2760352500924	39.0	37.0	39.0	34.0	39.0
13	37.333207889306394	39.0	37.0	39.0	34.0	39.0
14	38.541549564186276	40.0	38.0	41.0	35.0	41.0
15	38.53639641339165	40.0	38.0	41.0	34.0	41.0
16	38.50959406184551	40.0	38.0	41.0	34.0	41.0
17	38.52483502987557	40.0	38.0	41.0	34.0	41.0
18	38.47190406708143	40.0	38.0	41.0	34.0	41.0
19	38.529344277442405	40.0	38.0	41.0	34.0	41.0
20	38.49504704632253	40.0	38.0	41.0	34.0	41.0
21	38.46634955956634	40.0	38.0	41.0	34.0	41.0
22	38.446435721325614	40.0	38.0	41.0	34.0	41.0
23	38.39419582358014	40.0	38.0	41.0	34.0	41.0
24	38.34303198533941	40.0	38.0	41.0	34.0	41.0
25	38.25671045336947	40.0	38.0	41.0	34.0	41.0
26	38.12412317512628	40.0	38.0	41.0	34.0	41.0
27	38.06750011549833	40.0	38.0	41.0	34.0	41.0
28	37.96160739035358	40.0	37.0	41.0	33.0	41.0
29	37.86496993193298	40.0	37.0	41.0	33.0	41.0
30	37.81611317297031	40.0	37.0	41.0	33.0	41.0
31	37.723781877540965	40.0	37.0	41.0	33.0	41.0
32	37.62394511519034	39.0	37.0	41.0	33.0	41.0
33	37.57209117438709	39.0	37.0	41.0	33.0	41.0
34	37.51912459960577	39.0	37.0	41.0	32.0	41.0
35	37.522597249599606	39.0	37.0	41.0	32.0	41.0
36	37.43967425619071	39.0	37.0	40.0	32.0	41.0
37	37.42143803129235	39.0	37.0	40.0	32.0	41.0
38	37.38726881082913	39.0	37.0	40.0	32.0	41.0
39	37.31418146328693	39.0	36.0	40.0	32.0	41.0
40	37.277941935136134	39.0	36.0	40.0	32.0	41.0
41	37.2171195484785	39.0	36.0	40.0	31.0	41.0
42	37.18987445330787	39.0	36.0	40.0	31.0	41.0
43	36.05520146759887	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	3.0
17	9.0
18	22.0
19	64.0
20	107.0
21	239.0
22	453.0
23	734.0
24	1264.0
25	2086.0
26	3219.0
27	4876.0
28	6851.0
29	9799.0
30	13454.0
31	18095.0
32	24044.0
33	32179.0
34	44936.0
35	63545.0
36	96760.0
37	164172.0
38	317128.0
39	234933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.067693575212516	16.4906600344955	12.140992669705557	28.30065372058642
2	19.288318498213624	19.55598589380313	34.41272945669582	26.742966151287423
3	20.56034018110139	20.97969539238635	29.349956110632007	29.110008315880254
4	16.54186429715412	14.436714611309597	31.822294258962668	37.19912683257361
5	16.720309227547123	35.20581803622028	32.330679438216094	15.743193298016509
6	38.114162406061354	31.782254835530367	14.303891523962056	15.799691234446222
7	30.90427497844031	30.060655876555376	19.473500831588026	19.561568313416284
8	28.45089780707158	34.523607860046816	18.554134070469384	18.47136026241222
9	29.82263305408402	13.511091690279661	16.591528581988417	40.0747466736479
10	17.176912652457805	26.115040193421212	31.8374052913638	24.870641862757175
11	38.89579740051744	20.199889121596648	19.30015707773808	21.60415640014784
12	22.815541456203032	22.666163607244055	28.374476407539735	26.14381852901318
13	32.77400055439202	18.37848034988296	22.136026241222126	26.711492854502893
14	23.914026888012813	18.81689278674387	23.250103948503142	34.01897637674017
15	27.96310983121843	25.586924048293703	20.388728902303807	26.061237218184058
16	25.80232844647037	24.870353116915116	23.086000061599112	26.2413183750154
17	26.213598774177655	23.62325982505852	22.88253049156092	27.280610909202906
18	25.836881698903536	25.080945084390784	23.59332650609831	25.488846710607366
19	28.096414161636073	23.833274300850068	23.369163484045828	24.70114805346803
20	26.341320684982133	22.982244055685598	23.472726992731303	27.203708266600962
21	25.98837701737095	24.261965627694963	24.76871458051004	24.980942774424047
22	27.35934227547123	22.67877217568067	22.535361740790933	27.426523808057162
23	26.02081280029568	23.749056763582605	24.073799587285944	26.15633084883578
24	25.350056209190587	22.928633577676482	24.192474128372552	27.52883608476038
25	26.529294228163113	22.558557656769743	23.446836115559936	27.465311999507207
26	25.957673709498586	23.290624615005544	24.257634440064063	26.49406723543181
27	27.385136904028585	23.702183688554886	23.068867808303562	25.84381159911297
28	25.729083251201185	25.288168350375756	21.901660866083528	27.08108753233953
29	26.206572625354198	24.639452691881235	23.604876339780706	25.54909834298386
30	28.22018987926574	23.260787544659355	22.24767463348528	26.271347942589628
31	26.46644388320808	23.766189016878155	23.858395189109277	25.908971910804485
32	26.112633978070715	23.726630836515955	23.393033140322782	26.76770204509055
33	25.85362895774301	23.02199473327584	23.536924818282618	27.587451490698534
34	26.483672385117657	23.52883993470494	24.717702815079463	25.269784865097943
35	26.47019757915486	22.831422477516323	23.729037051866452	26.96934289146236
36	25.810894573118148	22.742585006775904	24.8596695207589	26.58685089934705
37	25.023484661820873	22.885995441665642	25.658148022668474	26.432371873845018
38	26.424671984723417	21.71031862141185	24.728771405691756	27.13623798817297
39	24.359850468153258	22.95962563139091	26.458166502402364	26.222357398053468
40	26.661732321054576	21.29981828261673	25.960753665147223	26.07769573118147
41	24.83801358260441	22.5159195207589	26.07519326721695	26.57087362941974
42	24.453692866822717	22.41052728840705	26.65326244302082	26.482517401749416
43	23.6723466182087	21.90021713687323	25.96758731674264	28.459848928175436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	20.0
2	26.0
3	52.0
4	78.0
5	78.0
6	100.0
7	122.0
8	122.0
9	122.0
10	169.5
11	217.0
12	217.0
13	337.0
14	457.0
15	648.0
16	839.0
17	822.5
18	806.0
19	806.0
20	976.0
21	1146.0
22	1341.5
23	1537.0
24	2080.0
25	2623.0
26	2623.0
27	3424.0
28	4225.0
29	5254.0
30	6283.0
31	8212.0
32	10141.0
33	10141.0
34	12887.5
35	15634.0
36	18709.0
37	21784.0
38	26174.0
39	30564.0
40	30564.0
41	35877.5
42	41191.0
43	48131.5
44	55072.0
45	65874.5
46	76677.0
47	76677.0
48	88743.0
49	100809.0
50	109146.5
51	117484.0
52	117903.0
53	118322.0
54	118322.0
55	110848.0
56	103374.0
57	96141.5
58	88909.0
59	83331.5
60	77754.0
61	77754.0
62	67755.5
63	57757.0
64	48441.5
65	39126.0
66	32411.5
67	25697.0
68	25697.0
69	20915.5
70	16134.0
71	13109.5
72	10085.0
73	8119.5
74	6154.0
75	6154.0
76	4982.0
77	3810.0
78	2958.5
79	2107.0
80	1589.5
81	1072.0
82	1072.0
83	786.5
84	501.0
85	348.0
86	195.0
87	139.0
88	83.0
89	83.0
90	58.0
91	33.0
92	19.5
93	6.0
94	5.0
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1038976.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.94748716977615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8946255714297	60.081132201103046
2	7.950578384437026	11.122459590754172
3	2.4859996449548736	5.216682848086472
4	1.178961611476261	3.2986160876957755
5	0.6934103878563352	2.425115710398526
6	0.43924417115899583	1.8434415615925692
7	0.29196053833813446	1.429533420664132
8	0.19515680110756758	1.0920582273252912
9	0.15267748054586794	0.9611465500436261
>10	0.6618242152260914	7.662301377559046
>50	0.03227219706261786	1.554943056427106
>100	0.02232436431745429	2.8331213111149296
>500	8.26827505048438E-4	0.3771773719263136
>1k	1.378045841747397E-4	0.10227068530892376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTA	1061	0.10211977947517557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.624861401995812E-5	0.0
6	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
7	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
8	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
9	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
10	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
11	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
12	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
13	0.0	0.0	0.0	9.624861401995812E-5	9.624861401995812E-5
14	0.0	0.0	0.0	1.9249722803991624E-4	9.624861401995812E-5
15	0.0	0.0	0.0	2.8874584205987433E-4	9.624861401995812E-5
16	0.0	0.0	0.0	2.8874584205987433E-4	9.624861401995812E-5
17	0.0	0.0	0.0	4.8124307009979057E-4	9.624861401995812E-5
18	9.624861401995812E-5	0.0	0.0	5.774916841197487E-4	9.624861401995812E-5
19	9.624861401995812E-5	0.0	0.0	5.774916841197487E-4	9.624861401995812E-5
20	9.624861401995812E-5	0.0	0.0	7.69988912159665E-4	9.624861401995812E-5
21	9.624861401995812E-5	0.0	0.0	0.0011549833682394973	9.624861401995812E-5
22	9.624861401995812E-5	0.0	0.0	0.001732475052359246	9.624861401995812E-5
23	9.624861401995812E-5	0.0	0.0	0.0023099667364789946	1.9249722803991624E-4
24	9.624861401995812E-5	0.0	0.0	0.0037536959467783662	1.9249722803991624E-4
25	9.624861401995812E-5	0.0	0.0	0.004234939016878157	1.9249722803991624E-4
26	9.624861401995812E-5	0.0	0.0	0.00615991129727732	1.9249722803991624E-4
27	9.624861401995812E-5	0.0	0.0	0.015399778243193298	1.9249722803991624E-4
28	9.624861401995812E-5	0.0	0.0	0.04956803622027843	1.9249722803991624E-4
29	9.624861401995812E-5	0.0	0.0	0.10789469631637305	1.9249722803991624E-4
30	9.624861401995812E-5	0.0	0.0	0.18508608476037944	1.9249722803991624E-4
31	9.624861401995812E-5	0.0	0.0	0.5218599852162129	1.9249722803991624E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	25	0.005496103	29.6	2
CTTATAC	485	0.0	20.979382	37
GATATAT	55	5.142636E-4	20.181818	1
ATACCGA	55	5.142636E-4	20.181818	6
TCTTATA	1045	0.0	18.942585	37
TATACCG	50	0.0070344377	18.5	5
TACTTAC	440	0.0	17.65909	31
GGTATCA	800	0.0	17.575	1
GTCTAGA	95	3.6057136E-6	17.526316	1
GCTCTAT	75	2.0670252E-4	17.266666	1
GTGTAGA	175	7.2759576E-12	16.914286	1
TAAGATT	110	7.805811E-7	16.818182	4
TCGTTAC	535	0.0	16.252337	23
AGGGCGT	560	0.0	16.1875	10
GTACTTA	510	0.0	15.960784	30
GGGCGTC	535	0.0	15.560747	11
GGCGTCA	610	0.0	15.467213	12
GTCCTAT	85	5.363533E-4	15.235294	1
ACTGTCG	85	5.363533E-4	15.235294	8
CGTTACG	450	0.0	15.211111	24
>>END_MODULE