FastQCFastQC Report
Thu 9 Feb 2017
SRR2727503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877027
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35090.18694456712663163No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT32970.17565011052051996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30440.16217134862737723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26700.14224622235055756No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG21070.1122519814579119No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT19500.1038876904807443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11850.019.67088737
TAGTACT1502.5102054E-1017.2666664
TCTTATA20800.017.25480837
AACGTTA700.002593313315.85714312
TACTTAC4550.014.637362531
GTACTTA5050.013.92079330
CCGAACG800.0063005813.8759
TCTATAC1601.7850289E-713.8753
GTATAGG1501.3066438E-613.5666661
TAGAACC2202.8558134E-1013.4545454
CTAGTAC1102.4576593E-413.4545453
TAATACT2355.638867E-1113.3829794
GAATATA1805.1635652E-813.3611111
AGGCGTG1951.0268195E-813.2820527
GCCTAAA1409.565731E-613.2142861
CCCTATA1000.001833232112.9500012
TTAGAAC2001.4639227E-812.9500013
ATTATAC2001.4639227E-812.9500013
GTACGTA1000.001833232112.9500011
TCGTTAC3900.012.80769323
Produced by FastQC (version 0.11.5)