FastQCFastQC Report
Thu 9 Feb 2017
SRR2727502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825213
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT30990.1697884027781963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.15904993006295703No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG24910.13647722211051533No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG20690.11335663289709201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20240.11089116722267484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18660.10223464329916564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9750.020.87179637
TATACTG1655.4569682E-1116.8181825
TCTTATA16650.016.33333437
GTATTAT803.3839289E-416.18751
CGACTTA1455.3505573E-815.31034619
ATAACGC855.366297E-415.2352943
TACTTAC4850.014.49484531
CGCAATG1152.2111424E-514.4782618
GTACTTA5250.014.447618530
TACGGAT9250.014.227
GTCTTAG2900.014.0344841
GGGTATA1356.5715612E-613.7037041
TTAGACT1356.5715612E-613.7037044
CTTATAG2304.0017767E-1113.6739122
ACGGATG9550.013.56020928
TCGTTAC5750.013.51304223
TAAGACT2202.8376235E-1013.4545454
GTTACGG8050.013.32919325
GCTTATA2254.0745363E-1013.1555561
CGTTACG4950.013.08080924
Produced by FastQC (version 0.11.5)