##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727502.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1825213 Sequences flagged as poor quality 0 Sequence length 43 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.062615705673803 31.0 31.0 33.0 28.0 34.0 2 31.23559661255974 31.0 31.0 34.0 28.0 34.0 3 31.334889681368693 31.0 31.0 34.0 28.0 34.0 4 35.19168173796702 37.0 35.0 37.0 32.0 37.0 5 34.130755698102085 35.0 35.0 37.0 30.0 37.0 6 34.08313166737252 35.0 33.0 37.0 30.0 37.0 7 34.09111977615763 35.0 33.0 37.0 30.0 37.0 8 33.977167596329856 35.0 33.0 37.0 30.0 37.0 9 35.54640417310199 37.0 35.0 39.0 30.0 39.0 10 35.222415685183044 37.0 34.0 39.0 30.0 39.0 11 35.43038867244535 37.0 34.0 39.0 30.0 39.0 12 35.35585490570141 37.0 34.0 39.0 30.0 39.0 13 35.50451098036229 37.0 35.0 39.0 30.0 39.0 14 36.413244919907974 38.0 35.0 40.0 31.0 41.0 15 36.345158619843275 38.0 35.0 40.0 31.0 41.0 16 36.296636063845696 38.0 35.0 40.0 30.0 41.0 17 36.304488298078084 38.0 35.0 40.0 30.0 41.0 18 36.24574556503816 38.0 35.0 40.0 30.0 41.0 19 36.31260351531575 38.0 35.0 40.0 30.0 41.0 20 36.29379694315129 38.0 35.0 40.0 30.0 41.0 21 36.238006742226794 38.0 35.0 40.0 30.0 41.0 22 36.23013040121893 38.0 35.0 40.0 30.0 41.0 23 36.14193028430107 38.0 34.0 40.0 30.0 41.0 24 36.04783934806513 38.0 34.0 40.0 29.0 41.0 25 35.93804887429577 38.0 34.0 40.0 29.0 41.0 26 35.73840094279407 38.0 34.0 40.0 29.0 41.0 27 35.618176618290576 38.0 34.0 40.0 28.0 41.0 28 35.4808058018434 38.0 34.0 40.0 27.0 41.0 29 35.30489153868617 38.0 34.0 40.0 27.0 40.0 30 35.203790461715975 38.0 34.0 40.0 27.0 40.0 31 35.08422085531935 38.0 33.0 39.0 27.0 40.0 32 34.93008596804866 37.0 33.0 39.0 26.0 40.0 33 34.85892057529724 37.0 33.0 39.0 26.0 40.0 34 34.77670715691812 37.0 33.0 39.0 26.0 40.0 35 34.758278622823745 37.0 33.0 39.0 26.0 40.0 36 34.66320423972435 37.0 33.0 39.0 25.0 40.0 37 34.61447458461012 37.0 33.0 39.0 25.0 40.0 38 34.57534545283208 37.0 33.0 39.0 25.0 40.0 39 34.48074115185461 37.0 33.0 39.0 25.0 40.0 40 34.39527824971661 37.0 33.0 39.0 25.0 40.0 41 34.33179524800667 37.0 33.0 39.0 25.0 40.0 42 34.26324708403896 37.0 33.0 39.0 25.0 40.0 43 33.110705435475204 36.0 31.0 38.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 2.0 12 1.0 13 7.0 14 10.0 15 36.0 16 70.0 17 139.0 18 271.0 19 565.0 20 1086.0 21 1920.0 22 3211.0 23 5136.0 24 8234.0 25 12417.0 26 18077.0 27 26010.0 28 35891.0 29 48946.0 30 65411.0 31 85381.0 32 110451.0 33 142352.0 34 182013.0 35 227607.0 36 270527.0 37 282655.0 38 227673.0 39 69111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40562608309277 18.284934415873654 12.799656807178122 26.509782693855456 2 18.694311294079103 21.043845293672575 34.972959320364254 25.28888409188407 3 20.827103466828255 22.786436432350634 29.31312674191998 27.073333358901124 4 16.269717561731152 15.29218781588779 33.125448920208214 35.31264570217284 5 16.215696469398367 35.596612559739604 32.81195126267455 15.375739708187483 6 36.45667656322851 33.49044741627416 14.538905870164195 15.51397015033314 7 30.100048597067847 30.243155182436244 20.4506542524078 19.206141968088108 8 27.62839186440158 35.118367007028766 19.122535287662316 18.130705840907336 9 28.539573189540068 14.488500794153888 17.704563796115853 39.26736222019019 10 16.873701863837262 26.767889555903885 32.42870832061792 23.929700259640928 11 37.31049472034223 21.250780045945323 20.130253290985763 21.308471942726683 12 22.393769932605124 23.955505467033163 29.386652407143714 24.264072193218 13 31.989526701815073 19.15962684903077 23.369875187169935 25.48097126198422 14 23.314429603558597 19.446168748524144 24.862468106462096 32.37693354145516 15 26.94343071192239 26.4072193217997 21.535842666034046 25.113507300243864 16 25.34317912484735 25.2851037111833 24.168138184420123 25.203578979549235 17 25.182156822244856 25.209715249672232 24.460268472775507 25.147859455307408 18 25.07279972255293 25.123643103572018 25.297376251429288 24.506180922445765 19 26.37768852183279 24.992096812810342 24.890848355780943 23.739366309575924 20 25.40908924054343 24.292068925654156 24.85288018439492 25.445961649407494 21 24.850579083098793 25.434675295431276 25.380928143728976 24.333817477740954 22 25.781648498010917 24.424930131442192 24.02612736157369 25.767294008973202 23 25.196730463786963 24.415342209375016 24.864495267127726 25.523432059710295 24 24.72325147804667 24.437093095435987 24.983385500760733 25.85626992575661 25 25.516857484578516 23.970791354214548 24.42569716520757 26.08665399599937 26 25.12369789169812 24.318038497424684 25.20549656396267 25.352767046914526 27 26.0650126861906 24.81376146236083 24.461145082793077 24.660080768655494 28 25.407609961138782 25.552195825911827 23.794757105061162 25.24543710788823 29 25.361587935216328 25.263626765752818 24.944705083735432 24.430080215295423 30 26.126430175546634 24.604032515657074 24.312011803553887 24.9575255052424 31 25.442290844958915 24.528424901641614 24.94777321879693 25.08151103460254 32 24.996096346015506 24.69355631370147 24.69415898308855 25.616188357194474 33 25.14714720966813 24.295958882607128 24.61104539579764 25.9458485119271 34 25.60769619764926 24.593129678563542 25.295623031394143 24.503551092393053 35 25.511488248220893 24.327407266987468 24.944924236239824 25.21618024855181 36 25.15794047050947 24.33754307031563 25.24384825223138 25.26066820694352 37 24.228459911254195 24.07488879380105 25.929028557214966 25.767622737729788 38 25.36640929031297 23.48613559075023 25.45708363900542 25.690371479931386 39 24.02546990406051 23.842587139144854 26.548079593998068 25.58386336279656 40 25.353314928175507 23.008437919300377 26.75742502381914 24.88082212870498 41 24.5675984118018 23.307033206535348 26.57519971641666 25.550168665246193 42 23.931343903423873 23.47632851617866 27.163788555089184 25.42853902530828 43 23.65225318907985 22.992987667740696 26.832484756573617 26.522274386605837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 49.0 1 77.0 2 105.0 3 255.0 4 405.0 5 405.0 6 564.0 7 723.0 8 728.5 9 734.0 10 973.0 11 1212.0 12 1212.0 13 2102.5 14 2993.0 15 4551.0 16 6109.0 17 5681.0 18 5253.0 19 5253.0 20 5979.0 21 6705.0 22 6812.0 23 6919.0 24 8580.5 25 10242.0 26 10242.0 27 12787.5 28 15333.0 29 18127.0 30 20921.0 31 25774.5 32 30628.0 33 30628.0 34 36786.0 35 42944.0 36 49785.5 37 56627.0 38 64967.5 39 73308.0 40 73308.0 41 82384.5 42 91461.0 43 101556.0 44 111651.0 45 124636.0 46 137621.0 47 137621.0 48 153051.5 49 168482.0 50 178544.5 51 188607.0 52 191396.0 53 194185.0 54 194185.0 55 179916.0 56 165647.0 57 150074.0 58 134501.0 59 124245.5 60 113990.0 61 113990.0 62 99037.0 63 84084.0 64 70419.0 65 56754.0 66 47211.5 67 37669.0 68 37669.0 69 30872.5 70 24076.0 71 19455.5 72 14835.0 73 11840.0 74 8845.0 75 8845.0 76 7092.0 77 5339.0 78 4193.5 79 3048.0 80 2428.0 81 1808.0 82 1808.0 83 1327.5 84 847.0 85 601.0 86 355.0 87 251.0 88 147.0 89 147.0 90 91.5 91 36.0 92 22.0 93 8.0 94 6.5 95 5.0 96 5.0 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1825213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.33751048551936 #Duplication Level Percentage of deduplicated Percentage of total 1 84.79163064558377 56.248656870356974 2 8.276582663855207 10.980957784955251 3 2.6299565019012086 5.233943010639937 4 1.278710504451406 3.393058859879555 5 0.7815632241755882 2.59234792894222 6 0.4685076280692678 1.8647777813754511 7 0.3518913739331851 1.6340518395639534 8 0.2638858302359543 1.4004423224206077 9 0.18844262599802808 1.1250733210256691 >10 0.9175616552805558 10.101927579959098 >50 0.031330994854147434 1.4033209842958825 >100 0.017620579175634796 2.309208314372685 >500 0.001488710884010331 0.654596793187626 >1k 8.270616022279595E-4 1.0576366090250473 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT 3099 0.1697884027781963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2903 0.15904993006295703 No Hit GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG 2491 0.13647722211051533 No Hit GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG 2069 0.11335663289709201 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2024 0.11089116722267484 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 1866 0.10223464329916564 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.47881260981595E-5 7 0.0 0.0 0.0 0.0 5.47881260981595E-5 8 0.0 0.0 0.0 0.0 5.47881260981595E-5 9 0.0 0.0 0.0 1.6436437829447852E-4 1.09576252196319E-4 10 0.0 0.0 0.0 1.6436437829447852E-4 1.6436437829447852E-4 11 0.0 0.0 0.0 1.6436437829447852E-4 1.6436437829447852E-4 12 0.0 0.0 0.0 1.6436437829447852E-4 1.6436437829447852E-4 13 0.0 0.0 0.0 1.6436437829447852E-4 1.6436437829447852E-4 14 0.0 0.0 0.0 2.739406304907975E-4 2.739406304907975E-4 15 0.0 0.0 0.0 3.2872875658895704E-4 2.739406304907975E-4 16 1.09576252196319E-4 0.0 0.0 4.930931348834355E-4 2.739406304907975E-4 17 1.09576252196319E-4 0.0 0.0 5.47881260981595E-4 2.739406304907975E-4 18 1.09576252196319E-4 0.0 0.0 6.574575131779141E-4 3.835168826871165E-4 19 1.09576252196319E-4 0.0 0.0 9.313981436687115E-4 3.835168826871165E-4 20 1.09576252196319E-4 0.0 0.0 0.00109576252196319 3.835168826871165E-4 21 1.09576252196319E-4 0.0 0.0 0.0015888556568466256 4.38305008785276E-4 22 1.09576252196319E-4 0.0 0.0 0.0020271606656319017 4.38305008785276E-4 23 1.09576252196319E-4 0.0 0.0 0.002739406304907975 5.47881260981595E-4 24 1.09576252196319E-4 0.0 0.0 0.0038899569529693245 5.47881260981595E-4 25 1.09576252196319E-4 0.0 0.0 0.005150083853226993 5.47881260981595E-4 26 1.09576252196319E-4 0.0 0.0 0.007177244518858895 5.47881260981595E-4 27 1.09576252196319E-4 0.0 0.0 0.01654601408164417 5.47881260981595E-4 28 1.09576252196319E-4 0.0 0.0 0.04602202592245398 5.47881260981595E-4 29 1.09576252196319E-4 0.0 0.0 0.09330417874516564 5.47881260981595E-4 30 1.09576252196319E-4 0.0 0.0 0.1527492955616687 5.47881260981595E-4 31 1.09576252196319E-4 0.0 0.0 0.4341411112018159 6.026693870797545E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 975 0.0 20.871796 37 TATACTG 165 5.4569682E-11 16.818182 5 TCTTATA 1665 0.0 16.333334 37 GTATTAT 80 3.3839289E-4 16.1875 1 CGACTTA 145 5.3505573E-8 15.310346 19 ATAACGC 85 5.366297E-4 15.235294 3 TACTTAC 485 0.0 14.494845 31 CGCAATG 115 2.2111424E-5 14.47826 18 GTACTTA 525 0.0 14.4476185 30 TACGGAT 925 0.0 14.2 27 GTCTTAG 290 0.0 14.034484 1 GGGTATA 135 6.5715612E-6 13.703704 1 TTAGACT 135 6.5715612E-6 13.703704 4 CTTATAG 230 4.0017767E-11 13.673912 2 ACGGATG 955 0.0 13.560209 28 TCGTTAC 575 0.0 13.513042 23 TAAGACT 220 2.8376235E-10 13.454545 4 GTTACGG 805 0.0 13.329193 25 GCTTATA 225 4.0745363E-10 13.155556 1 CGTTACG 495 0.0 13.080809 24 >>END_MODULE