FastQCFastQC Report
Thu 9 Feb 2017
SRR2727501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599066
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.19611448182876756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24640.15408995000831735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21410.13389065867199978No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT20960.13107651591616606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG400.001931516223.1255
CTTATAC8200.018.27439137
CGACAAA650.001580299817.07692314
TACGCTG904.4477067E-516.4444455
GTATTAC1059.347596E-615.8571421
CTATTAC1701.4879333E-915.2352941
TATACTG1701.4879333E-915.2352945
GGACCGT1101.4524954E-515.1363646
ATTACGG2450.014.34693825
TACGGAT6250.014.20827
TCTTATA13550.014.06273137
CATTACG2650.013.96226424
TATAGGA1753.5679477E-813.7428572
GTTACGG5000.013.6925
ACGGATG7050.013.645390528
ATTATAC1501.3062963E-613.5666673
GGGCGTC4100.013.53658611
ATAGAGT1102.457302E-413.4545456
TTACGGG1254.846698E-513.3226
TACACCG1254.846698E-513.325
Produced by FastQC (version 0.11.5)