##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727501.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1599066
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310402447428686	31.0	31.0	34.0	30.0	34.0
2	31.485471518999216	31.0	31.0	34.0	30.0	34.0
3	31.584408023183535	31.0	31.0	34.0	30.0	34.0
4	35.411790382635864	37.0	35.0	37.0	33.0	37.0
5	34.532947358020245	35.0	35.0	37.0	30.0	37.0
6	34.49171641445694	35.0	35.0	37.0	30.0	37.0
7	34.48206077797914	35.0	35.0	37.0	30.0	37.0
8	34.404344786268986	35.0	35.0	37.0	30.0	37.0
9	35.99830963825133	37.0	35.0	39.0	32.0	39.0
10	35.722397949803195	37.0	35.0	39.0	30.0	39.0
11	35.89237967663624	37.0	35.0	39.0	31.0	39.0
12	35.837330041411676	37.0	35.0	39.0	30.0	39.0
13	35.9529788013753	37.0	35.0	39.0	31.0	39.0
14	36.92472230664651	38.0	36.0	40.0	32.0	41.0
15	36.87213348292065	38.0	36.0	40.0	31.0	41.0
16	36.836501432711344	38.0	36.0	40.0	31.0	41.0
17	36.84058068897719	38.0	36.0	40.0	31.0	41.0
18	36.788275155622095	38.0	36.0	40.0	31.0	41.0
19	36.862334012479785	38.0	36.0	40.0	31.0	41.0
20	36.840994680644826	38.0	36.0	40.0	31.0	41.0
21	36.78963094706535	38.0	36.0	40.0	31.0	41.0
22	36.77334894244515	38.0	36.0	40.0	31.0	41.0
23	36.68692036476293	38.0	36.0	40.0	31.0	41.0
24	36.6055197221378	38.0	35.0	40.0	31.0	41.0
25	36.507511885062904	38.0	35.0	40.0	30.0	41.0
26	36.327262289361414	38.0	35.0	40.0	30.0	41.0
27	36.22007471861699	38.0	35.0	40.0	30.0	41.0
28	36.104787419656226	38.0	35.0	40.0	30.0	41.0
29	35.95398876594212	38.0	34.0	40.0	29.0	41.0
30	35.85753683712868	38.0	34.0	40.0	29.0	41.0
31	35.74634192709995	38.0	34.0	40.0	29.0	41.0
32	35.59201496373508	38.0	34.0	40.0	28.0	41.0
33	35.523084100343574	38.0	34.0	40.0	28.0	41.0
34	35.434510520516355	38.0	34.0	40.0	27.0	41.0
35	35.42498683606555	38.0	34.0	40.0	27.0	41.0
36	35.334589691732546	38.0	34.0	40.0	27.0	41.0
37	35.277915983455344	38.0	34.0	40.0	27.0	41.0
38	35.231533907918745	38.0	34.0	40.0	27.0	41.0
39	35.15366782859494	38.0	33.0	40.0	27.0	41.0
40	35.06471652827338	38.0	33.0	40.0	27.0	41.0
41	34.99619215216883	38.0	33.0	40.0	27.0	40.0
42	34.927322574552896	38.0	33.0	40.0	26.0	40.0
43	33.80804482116435	36.0	31.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	5.0
12	0.0
13	2.0
14	9.0
15	19.0
16	37.0
17	99.0
18	179.0
19	356.0
20	681.0
21	1233.0
22	2033.0
23	3477.0
24	5408.0
25	8271.0
26	12327.0
27	17422.0
28	24382.0
29	33835.0
30	45650.0
31	60016.0
32	79626.0
33	105513.0
34	140381.0
35	186898.0
36	239802.0
37	278316.0
38	257225.0
39	95859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49092907985037	18.46165198934878	12.771205191030264	26.276213739770593
2	18.520123622164437	20.892571038343632	35.34113038486216	25.246174954629762
3	20.442808489455718	22.73358322921005	29.429304356418058	27.39430392491617
4	15.749881493321727	15.3994269154619	33.64757927440144	35.20311231681494
5	15.92354536960951	35.70202855917142	33.228021857759465	15.146404213459608
6	36.146350432064715	33.97677144032829	14.732412545823625	15.144465581783367
7	30.06323691454887	30.3275161875745	20.683699109355086	18.92554778852155
8	27.32101114025312	35.26346004480116	19.187450674331142	18.228078140614585
9	28.00415992835818	14.599835153771012	18.190993992743266	39.205010925127546
10	16.7670377582914	26.837979170340688	32.77350653443948	23.621476536928434
11	37.00947928353176	21.283048979841983	20.735479336062426	20.97199240056383
12	22.18294929665192	24.094940421471033	29.752555554304827	23.96955472757222
13	31.55504525766916	19.41101868215571	23.800268406682402	25.233667653492724
14	23.084850781643784	19.654660908305225	24.985335189416823	32.27515312063417
15	26.2204311766994	26.761622096899064	21.87014169521458	25.147805031186955
16	25.10796927706549	25.56267220990253	24.199814141505104	25.12954437152688
17	24.861012616114657	25.440976169839143	24.882650247081735	24.815360966964466
18	24.837498889977024	25.258619719261116	25.532842296690696	24.371039094071165
19	25.88679891886889	25.120226432179788	25.31665359653698	23.676321052414348
20	25.10896986115645	24.651765468091998	25.238107745396377	25.00115692535518
21	24.765144152899254	25.280444959745253	25.685556443573937	24.268854443781557
22	25.460299950095866	24.575033175616266	24.694352828463614	25.27031404582425
23	24.929114870805833	24.761266889546775	25.221910790423912	25.087707449223483
24	24.572719324905915	24.73462633812488	25.231103656759636	25.461550680209573
25	25.276630232898455	24.255096412530815	25.008348623508976	25.459924731061754
26	24.9089781159752	24.58241248328712	25.48068685094924	25.02792254978844
27	25.577305752232864	25.071322884733966	24.81579872250426	24.53557264052891
28	25.206776956048095	25.442664655492642	24.450898211831156	24.899660176628107
29	24.9621966823133	25.457861026374147	25.256806160596245	24.323136130716307
30	25.616453604791793	24.988399478195397	24.777151161990812	24.617995755021994
31	25.203900276786573	24.693852536418134	25.18676527422883	24.915481912566463
32	24.66433530573472	25.050248082318056	25.10628079141199	25.179135820535237
33	24.89065491980944	24.545078189393056	25.09064666499069	25.473620225806815
34	25.279444375654286	24.749947782017752	25.641718353088617	24.328889489239344
35	25.1594368212444	24.714114364260137	25.266686928494508	24.859761886000953
36	24.952378450920726	24.535385031011852	25.522773919275377	24.989462598792045
37	24.266540593071205	24.41506479407354	26.06208874430449	25.256305868550765
38	24.949501771659204	23.97330691791333	25.825450606791716	25.25174070363575
39	24.068424943060513	24.250718857132853	26.52216981663046	25.158686383176175
40	24.96413531398954	23.714843539916426	26.66838016692244	24.652640979171593
41	24.352278142365606	23.911333240779307	26.585831979417986	25.150556637437106
42	23.937848719189827	24.100818853005443	26.908770494776324	25.052561933028407
43	23.624165606672896	23.737481754974464	26.681825515644757	25.95652712270788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	164.5
2	201.0
3	401.5
4	602.0
5	602.0
6	844.5
7	1087.0
8	1012.0
9	937.0
10	1235.5
11	1534.0
12	1534.0
13	2541.0
14	3548.0
15	5179.0
16	6810.0
17	6395.0
18	5980.0
19	5980.0
20	6846.0
21	7712.0
22	7561.5
23	7411.0
24	9127.5
25	10844.0
26	10844.0
27	13144.5
28	15445.0
29	18355.5
30	21266.0
31	25259.5
32	29253.0
33	29253.0
34	34682.0
35	40111.0
36	46648.5
37	53186.0
38	60640.0
39	68094.0
40	68094.0
41	76382.0
42	84670.0
43	93452.5
44	102235.0
45	113176.0
46	124117.0
47	124117.0
48	134444.0
49	144771.0
50	150884.5
51	156998.0
52	157519.5
53	158041.0
54	158041.0
55	147619.0
56	137197.0
57	125439.5
58	113682.0
59	105351.0
60	97020.0
61	97020.0
62	84261.0
63	71502.0
64	60426.5
65	49351.0
66	41037.5
67	32724.0
68	32724.0
69	26720.0
70	20716.0
71	16929.0
72	13142.0
73	10646.5
74	8151.0
75	8151.0
76	6495.5
77	4840.0
78	3838.0
79	2836.0
80	2186.5
81	1537.0
82	1537.0
83	1160.5
84	784.0
85	597.0
86	410.0
87	272.5
88	135.0
89	135.0
90	87.0
91	39.0
92	25.5
93	12.0
94	7.5
95	3.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1599066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.8249509847387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.41127811100175	57.251908004378414
2	8.548357519029782	11.595838594564349
3	2.786961884887633	5.670766597165162
4	1.3476692675913946	3.6562240807210022
5	0.7383191965136676	2.5038231657315584
6	0.48784147766573255	1.9852694586600488
7	0.34518203747297294	1.6388368340691641
8	0.25799077223617184	1.3998569185146594
9	0.18756944065043452	1.144969930651552
>10	0.8506955647583411	9.4232629311208
>50	0.026127949208668375	1.2086869765035118
>100	0.010806202956211845	1.4202000292606443
>500	5.541120125940288E-4	0.2503002498276712
>1k	6.464640146930336E-4	0.8500562288316631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3136	0.19611448182876756	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2464	0.15408995000831735	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2141	0.13389065867199978	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2096	0.13107651591616606	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.253650568519374E-5
7	0.0	0.0	0.0	0.0	6.253650568519374E-5
8	0.0	0.0	0.0	0.0	1.2507301137038747E-4
9	0.0	0.0	0.0	6.253650568519374E-5	1.876095170555812E-4
10	0.0	0.0	0.0	6.253650568519374E-5	1.876095170555812E-4
11	0.0	0.0	0.0	6.253650568519374E-5	1.876095170555812E-4
12	0.0	6.253650568519374E-5	0.0	6.253650568519374E-5	1.876095170555812E-4
13	0.0	6.253650568519374E-5	0.0	6.253650568519374E-5	1.876095170555812E-4
14	0.0	6.253650568519374E-5	0.0	1.2507301137038747E-4	3.1268252842596867E-4
15	0.0	1.2507301137038747E-4	0.0	1.876095170555812E-4	3.1268252842596867E-4
16	0.0	1.2507301137038747E-4	0.0	3.1268252842596867E-4	3.1268252842596867E-4
17	0.0	1.2507301137038747E-4	0.0	3.752190341111624E-4	3.1268252842596867E-4
18	0.0	1.2507301137038747E-4	0.0	3.752190341111624E-4	3.752190341111624E-4
19	0.0	1.876095170555812E-4	0.0	4.377555397963561E-4	3.752190341111624E-4
20	0.0	1.876095170555812E-4	0.0	7.504380682223248E-4	3.752190341111624E-4
21	0.0	1.876095170555812E-4	0.0	0.0010631205966482934	3.752190341111624E-4
22	0.0	1.876095170555812E-4	0.0	0.0015008761364446496	4.377555397963561E-4
23	0.0	1.876095170555812E-4	0.0	0.0018135586648706183	6.253650568519373E-4
24	0.0	1.876095170555812E-4	0.0	0.002814142755833718	6.253650568519373E-4
25	0.0	1.876095170555812E-4	0.0	0.003939799858167205	6.253650568519373E-4
26	0.0	1.876095170555812E-4	0.0	0.00569082201735263	6.253650568519373E-4
27	0.0	1.876095170555812E-4	0.0	0.01138164403470526	6.253650568519373E-4
28	0.0	1.876095170555812E-4	0.0	0.03539566221781965	6.87901562537131E-4
29	0.0	1.876095170555812E-4	0.0	0.07254234659482472	6.87901562537131E-4
30	0.0	1.876095170555812E-4	0.0	0.12338452571688724	6.87901562537131E-4
31	0.0	1.876095170555812E-4	0.0	0.3758443991680143	6.87901562537131E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTCCG	40	0.0019315162	23.125	5
CTTATAC	820	0.0	18.274391	37
CGACAAA	65	0.0015802998	17.076923	14
TACGCTG	90	4.4477067E-5	16.444445	5
GTATTAC	105	9.347596E-6	15.857142	1
CTATTAC	170	1.4879333E-9	15.235294	1
TATACTG	170	1.4879333E-9	15.235294	5
GGACCGT	110	1.4524954E-5	15.136364	6
ATTACGG	245	0.0	14.346938	25
TACGGAT	625	0.0	14.208	27
TCTTATA	1355	0.0	14.062731	37
CATTACG	265	0.0	13.962264	24
TATAGGA	175	3.5679477E-8	13.742857	2
GTTACGG	500	0.0	13.69	25
ACGGATG	705	0.0	13.6453905	28
ATTATAC	150	1.3062963E-6	13.566667	3
GGGCGTC	410	0.0	13.536586	11
ATAGAGT	110	2.457302E-4	13.454545	6
TTACGGG	125	4.846698E-5	13.32	26
TACACCG	125	4.846698E-5	13.32	5
>>END_MODULE