FastQCFastQC Report
Thu 9 Feb 2017
SRR2727500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402262
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT14380.10254859648196986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7550.020.09271637
ACTATAC1156.407936E-817.6956523
GTAGTGT1705.456968E-1217.4117646
GGGTATA752.0676752E-417.2666661
TACTTAC2700.017.1296331
GTAATAC1005.879645E-616.6500023
GTATTAT1352.2202585E-816.4444451
CGTCTAA803.3832298E-416.187510
TTAGTAC957.0614435E-515.5789483
GTACTTA3000.015.41666730
TAGTACT1101.4522422E-515.1363644
TAATACT1853.0559022E-1015.04
CTAACAC1508.1041435E-814.83
TCTTATA12350.014.68016137
TCTACAC2059.276846E-1114.4390243
TTACATA1458.913212E-714.03448334
CGTGCAA800.006299533413.87510
GTATCGA1203.301377E-513.8749999
CTCTTAT19900.013.38693437
TATACTG1805.1599272E-813.3611115
Produced by FastQC (version 0.11.5)