##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727500.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1402262
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.527964816845923	31.0	30.0	31.0	27.0	34.0
2	30.691580460712764	31.0	30.0	33.0	27.0	34.0
3	30.799446893661813	31.0	31.0	34.0	27.0	34.0
4	34.61111261661515	35.0	35.0	37.0	32.0	37.0
5	33.11257240087801	35.0	32.0	37.0	26.0	37.0
6	33.07008818608791	35.0	32.0	37.0	26.0	37.0
7	33.097509595211164	35.0	32.0	37.0	26.0	37.0
8	32.96383913990395	35.0	32.0	37.0	26.0	37.0
9	34.42398995337533	37.0	33.0	39.0	28.0	39.0
10	34.01051657964061	37.0	32.0	39.0	27.0	39.0
11	34.25065287371404	37.0	32.0	39.0	27.0	39.0
12	34.1793409505499	37.0	32.0	39.0	27.0	39.0
13	34.362969259667594	37.0	33.0	39.0	27.0	39.0
14	35.14541291142454	37.0	33.0	39.0	27.0	40.0
15	35.10328526338159	37.0	33.0	39.0	27.0	40.0
16	35.05281751912268	37.0	33.0	39.0	27.0	40.0
17	35.04663607799398	37.0	33.0	39.0	27.0	40.0
18	34.96300976565007	37.0	33.0	39.0	27.0	40.0
19	35.03183000038509	37.0	33.0	39.0	27.0	40.0
20	34.990010426011686	37.0	33.0	39.0	27.0	40.0
21	34.92339020810662	37.0	33.0	39.0	27.0	40.0
22	34.90631707911931	37.0	33.0	39.0	27.0	40.0
23	34.798268083995715	37.0	33.0	39.0	27.0	40.0
24	34.68365897385795	37.0	33.0	39.0	26.0	40.0
25	34.56434460892472	37.0	32.0	39.0	26.0	40.0
26	34.324324555610865	37.0	32.0	39.0	25.0	40.0
27	34.16970794330874	37.0	32.0	39.0	25.0	40.0
28	34.02194882268791	37.0	32.0	39.0	24.0	40.0
29	33.82068757478987	36.0	31.0	39.0	24.0	40.0
30	33.70387060335372	36.0	31.0	39.0	24.0	40.0
31	33.56747526496475	36.0	31.0	39.0	24.0	40.0
32	33.3949982242976	36.0	31.0	39.0	24.0	40.0
33	33.29756422123683	36.0	31.0	39.0	23.0	40.0
34	33.20624961669075	36.0	30.0	39.0	23.0	40.0
35	33.1910113801843	36.0	30.0	39.0	23.0	40.0
36	33.09143369783963	36.0	30.0	38.0	23.0	40.0
37	33.02090764778622	36.0	30.0	38.0	23.0	40.0
38	32.95924798646758	35.0	30.0	38.0	22.0	40.0
39	32.85526884419602	35.0	30.0	38.0	22.0	40.0
40	32.74869389600517	35.0	30.0	38.0	21.0	40.0
41	32.67684213078583	35.0	30.0	38.0	21.0	40.0
42	32.61260449188526	35.0	30.0	38.0	21.0	40.0
43	31.460809035686626	34.0	28.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	7.0
13	26.0
14	53.0
15	100.0
16	219.0
17	448.0
18	812.0
19	1378.0
20	2479.0
21	4202.0
22	6419.0
23	9915.0
24	14972.0
25	20802.0
26	28220.0
27	38187.0
28	49727.0
29	62983.0
30	78341.0
31	93813.0
32	111501.0
33	127668.0
34	143376.0
35	154566.0
36	155708.0
37	140808.0
38	110686.0
39	44837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51784759196213	19.05749424857837	12.990225792326967	26.434432367132533
2	19.75294203223078	21.455405623200228	33.97004268817097	24.82160965639802
3	21.528073926270554	23.095612660116295	28.66040725627593	26.715906157337216
4	17.17688991072995	15.840905622487094	32.66778961420904	34.31441485257391
5	16.32006001731488	35.68748208252096	32.59733202497108	15.39512587519308
6	36.53882084802982	33.761522454434335	14.42476512948365	15.27489156805219
7	30.38184019819406	30.065922060214138	20.26689734158096	19.28534040001084
8	27.718571850338954	35.19477815130125	18.683027850715487	18.403622147644306
9	28.571408196185878	14.422055222205266	17.543226586757683	39.46330999485117
10	16.935208969507837	26.96329216651382	32.31871076874364	23.7827880952347
11	37.84977415062235	21.123085414851147	20.04040614378768	20.98673429073882
12	22.469624078809808	23.894963993889874	29.152611994049614	24.482799933250703
13	32.04793398095363	19.201832467826982	23.12834548750519	25.6218880637142
14	23.352839911514394	19.331765390490506	24.403642115382144	32.911752582612955
15	26.684528283587518	26.54946079976495	21.219215809884314	25.546795106763216
16	25.571041645569803	25.463857681374808	23.623402759256116	25.341697913799276
17	25.46014938720439	25.164484240462908	24.058770757533186	25.316595614799514
18	25.35210966281622	25.25212834691377	24.72405299437623	24.671708995893777
19	26.73451894153874	24.72590714146144	24.476880925247922	24.062692991751895
20	25.672520541810307	24.34915871641676	24.415337504688853	25.56298323708408
21	25.393827972233435	24.977928518351064	24.98470328654702	24.64354022286848
22	26.080646840604683	24.280056080817992	23.78984811682838	25.849448961748944
23	25.646776422665663	24.405282322419065	24.477736685441094	25.47020456947418
24	25.283506220663472	24.23905090489509	24.505263638321512	25.972179236119924
25	25.848379261507475	24.00057906439738	24.207031211000512	25.94401046309463
26	25.544013886135403	24.244328092752994	24.719346313313775	25.49231170779783
27	26.28531615347203	24.718847119867757	23.993305102755404	25.002531623904805
28	25.63836144743279	25.262112215834133	23.592738019000727	25.506788317732347
29	25.758096561127665	25.08967653690965	24.422326212933104	24.729900689029584
30	26.453473031430647	24.539565359397887	23.754120128763383	25.252841480408083
31	25.74711430531527	24.51446306039813	24.46418714904918	25.274235485237423
32	25.477050651019567	24.703586063089496	24.249533967261467	25.569829318629473
33	25.554354321802915	24.253884081576764	24.18941681369102	26.002344782929292
34	25.76394425578102	24.4763817318019	24.882226003414484	24.8774480090026
35	25.83083617754742	24.230493302963353	24.45541560706915	25.48325491242008
36	25.540519532013278	24.056845297098544	24.867178886684513	25.53545628420367
37	24.868248586925983	24.048572948564534	25.481899958780886	25.601278505728605
38	25.7547448337044	23.449683440041873	25.04738772069699	25.748184005556734
39	24.615086196445457	23.931547742148044	25.864068198382327	25.589297863024168
40	25.74354863784371	23.150880505925425	25.961696173753552	25.143874682477314
41	24.873383148085022	23.46266246963834	25.94992947109741	25.714024911179223
42	24.441366877231214	23.567635720000972	26.389005763544905	25.601991639222916
43	24.265080277437452	23.11158685038887	26.093697183550578	26.5296356886231
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	56.5
2	81.0
3	161.0
4	241.0
5	241.0
6	342.5
7	444.0
8	447.0
9	450.0
10	559.5
11	669.0
12	669.0
13	1122.0
14	1575.0
15	2259.0
16	2943.0
17	2839.5
18	2736.0
19	2736.0
20	3243.0
21	3750.0
22	4055.5
23	4361.0
24	5567.5
25	6774.0
26	6774.0
27	8606.5
28	10439.0
29	12643.0
30	14847.0
31	18417.0
32	21987.0
33	21987.0
34	26523.0
35	31059.0
36	36691.0
37	42323.0
38	48907.0
39	55491.0
40	55491.0
41	63237.0
42	70983.0
43	79466.0
44	87949.0
45	98146.5
46	108344.0
47	108344.0
48	118786.5
49	129229.0
50	135811.5
51	142394.0
52	143445.0
53	144496.0
54	144496.0
55	136647.5
56	128799.0
57	117757.0
58	106715.0
59	99181.0
60	91647.0
61	91647.0
62	79544.0
63	67441.0
64	56593.0
65	45745.0
66	38101.0
67	30457.0
68	30457.0
69	24900.0
70	19343.0
71	15623.5
72	11904.0
73	9585.5
74	7267.0
75	7267.0
76	5780.0
77	4293.0
78	3417.5
79	2542.0
80	1977.0
81	1412.0
82	1412.0
83	1027.5
84	643.0
85	463.5
86	284.0
87	201.5
88	119.0
89	119.0
90	81.5
91	44.0
92	25.0
93	6.0
94	5.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1402262.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.92513990030356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.99448753710753	63.440851741987814
2	7.338323352327099	10.702965142042364
3	2.2903155069175276	5.010647362733862
4	1.0960075454309124	3.197060143293504
5	0.6293616136488978	2.2948141861613336
6	0.3900270514966704	1.7065666377178552
7	0.28688984328261635	1.4645037370152671
8	0.19112603289865407	1.1150314150179494
9	0.13741802670563077	0.9019105940098595
>10	0.6159791354913987	7.168073526936827
>50	0.018903256908072635	0.9589483260854197
>100	0.010279989219544233	1.4497661262511559
>500	5.874057103332269E-4	0.3166341906416936
>1k	2.9370285516661343E-4	0.2722268701052487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	1438	0.10254859648196986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.131334943113342E-5	0.0
4	0.0	0.0	0.0	1.4262669886226683E-4	0.0
5	0.0	0.0	0.0	1.4262669886226683E-4	0.0
6	0.0	0.0	0.0	1.4262669886226683E-4	0.0
7	0.0	0.0	0.0	1.4262669886226683E-4	0.0
8	0.0	0.0	0.0	1.4262669886226683E-4	7.131334943113342E-5
9	0.0	0.0	0.0	1.4262669886226683E-4	1.4262669886226683E-4
10	0.0	0.0	0.0	1.4262669886226683E-4	1.4262669886226683E-4
11	0.0	0.0	0.0	1.4262669886226683E-4	1.4262669886226683E-4
12	0.0	0.0	0.0	1.4262669886226683E-4	1.4262669886226683E-4
13	0.0	0.0	0.0	1.4262669886226683E-4	1.4262669886226683E-4
14	0.0	0.0	0.0	1.4262669886226683E-4	1.4262669886226683E-4
15	0.0	0.0	0.0	2.1394004829340023E-4	2.1394004829340023E-4
16	0.0	0.0	0.0	2.1394004829340023E-4	2.1394004829340023E-4
17	0.0	0.0	0.0	4.991934460179338E-4	2.1394004829340023E-4
18	0.0	0.0	0.0	5.705067954490673E-4	2.8525339772453366E-4
19	0.0	0.0	0.0	6.418201448802007E-4	2.8525339772453366E-4
20	0.0	0.0	0.0	9.270735426047343E-4	2.8525339772453366E-4
21	0.0	0.0	0.0	0.0010697002414670012	2.8525339772453366E-4
22	0.0	0.0	0.0	0.0014975803380538016	2.8525339772453366E-4
23	0.0	0.0	0.0	0.0016402070369160684	4.2788009658680046E-4
24	0.0	0.0	0.0	0.002638593928951936	4.991934460179338E-4
25	0.0	0.0	0.0	0.0030664740255387365	4.991934460179338E-4
26	0.0	0.0	0.0	0.004635367713023672	4.991934460179338E-4
27	0.0	0.0	0.0	0.010483062366376611	4.991934460179338E-4
28	0.0	0.0	0.0	0.03344596088320157	4.991934460179338E-4
29	0.0	0.0	0.0	0.06710586181469654	4.991934460179338E-4
30	0.0	0.0	0.0	0.119735113694873	4.991934460179338E-4
31	0.0	0.0	0.0	0.3460123714398593	4.991934460179338E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	755	0.0	20.092716	37
ACTATAC	115	6.407936E-8	17.695652	3
GTAGTGT	170	5.456968E-12	17.411764	6
GGGTATA	75	2.0676752E-4	17.266666	1
TACTTAC	270	0.0	17.12963	31
GTAATAC	100	5.879645E-6	16.650002	3
GTATTAT	135	2.2202585E-8	16.444445	1
CGTCTAA	80	3.3832298E-4	16.1875	10
TTAGTAC	95	7.0614435E-5	15.578948	3
GTACTTA	300	0.0	15.416667	30
TAGTACT	110	1.4522422E-5	15.136364	4
TAATACT	185	3.0559022E-10	15.0	4
CTAACAC	150	8.1041435E-8	14.8	3
TCTTATA	1235	0.0	14.680161	37
TCTACAC	205	9.276846E-11	14.439024	3
TTACATA	145	8.913212E-7	14.034483	34
CGTGCAA	80	0.0062995334	13.875	10
GTATCGA	120	3.301377E-5	13.874999	9
CTCTTAT	1990	0.0	13.386934	37
TATACTG	180	5.1599272E-8	13.361111	5
>>END_MODULE