FastQCFastQC Report
Thu 9 Feb 2017
SRR2727499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975416
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13750.020.7237
GTACTTA10850.016.53917130
TCTTATA24650.016.43610637
TACTTAC10300.016.34466231
CTTACAT8350.014.40119733
CCTATAC2450.014.346943
AGTGTAC12650.014.33201627
ACTTACA10150.014.21674732
TAACGTT800.00630172313.8754
TTACATA7650.013.78431334
CGCGACA1102.4584177E-413.45454528
TATACTG3300.013.4545455
CTCTTAT39600.013.2209637
CTATACT3100.013.1290324
GTAAGAC3250.013.0923073
CCCTATA1703.7369318E-713.0588242
ATTATAC2551.6370905E-1113.0588243
ACATATA5400.013.01851836
GTCTTAG4000.012.9500011
GTCCTAC3000.012.951
Produced by FastQC (version 0.11.5)