##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727499.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2975416
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38884411457087	33.0	31.0	34.0	31.0	34.0
2	32.56777808548452	34.0	31.0	34.0	31.0	34.0
3	32.656923603287744	34.0	31.0	34.0	31.0	34.0
4	36.18345065026202	37.0	35.0	37.0	35.0	37.0
5	35.99671776988495	37.0	35.0	37.0	35.0	37.0
6	36.01301297028718	37.0	35.0	37.0	35.0	37.0
7	35.994564793628854	37.0	35.0	37.0	35.0	37.0
8	35.97394045067984	37.0	35.0	37.0	35.0	37.0
9	37.66024582781029	39.0	37.0	39.0	35.0	39.0
10	37.60060173098484	39.0	37.0	39.0	35.0	39.0
11	37.68640721163024	39.0	37.0	39.0	35.0	39.0
12	37.657134330123924	39.0	37.0	39.0	35.0	39.0
13	37.69421721197977	39.0	37.0	39.0	35.0	39.0
14	39.00592219709782	40.0	38.0	41.0	36.0	41.0
15	39.01188640512789	40.0	38.0	41.0	36.0	41.0
16	38.99384791908089	40.0	38.0	41.0	36.0	41.0
17	39.00663335815899	40.0	38.0	41.0	36.0	41.0
18	38.97420595977167	40.0	38.0	41.0	36.0	41.0
19	39.019393590677744	40.0	38.0	41.0	36.0	41.0
20	38.99367584230239	40.0	38.0	41.0	36.0	41.0
21	38.9693266420561	40.0	38.0	41.0	36.0	41.0
22	38.946110056543354	40.0	38.0	41.0	36.0	41.0
23	38.903432998948716	40.0	38.0	41.0	35.0	41.0
24	38.86255266490467	40.0	38.0	41.0	35.0	41.0
25	38.800747525724134	40.0	38.0	41.0	35.0	41.0
26	38.693664012023866	40.0	38.0	41.0	35.0	41.0
27	38.651381857192405	40.0	38.0	41.0	35.0	41.0
28	38.571910280780905	40.0	38.0	41.0	35.0	41.0
29	38.49134978100541	40.0	38.0	41.0	34.0	41.0
30	38.43704745823777	40.0	38.0	41.0	34.0	41.0
31	38.365620807308964	40.0	38.0	41.0	34.0	41.0
32	38.27183593823519	40.0	38.0	41.0	34.0	41.0
33	38.22925869861559	40.0	38.0	41.0	34.0	41.0
34	38.18409560209396	40.0	38.0	41.0	34.0	41.0
35	38.17251302002813	40.0	38.0	41.0	34.0	41.0
36	38.10325446929102	40.0	38.0	41.0	34.0	41.0
37	38.08835873706399	40.0	38.0	41.0	34.0	41.0
38	38.05364459961229	40.0	38.0	41.0	33.0	41.0
39	38.00243260102117	40.0	38.0	41.0	33.0	41.0
40	37.950507424844126	40.0	37.0	41.0	33.0	41.0
41	37.89813256364824	40.0	37.0	41.0	33.0	41.0
42	37.85997487410164	40.0	37.0	41.0	33.0	41.0
43	36.777767209694375	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	0.0
14	3.0
15	2.0
16	11.0
17	15.0
18	34.0
19	75.0
20	159.0
21	370.0
22	756.0
23	1297.0
24	2238.0
25	3702.0
26	5823.0
27	8737.0
28	12829.0
29	18749.0
30	26382.0
31	36653.0
32	49805.0
33	68942.0
34	98920.0
35	142339.0
36	219734.0
37	385530.0
38	892640.0
39	999668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27741062090141	16.808372341884294	12.216308576683058	27.697908460531234
2	18.512974320229507	19.56902833082836	35.67726328015981	26.240734068782313
3	20.217542689828917	21.4904067195982	29.35115627529058	28.9408943152823
4	15.581182597660293	14.499854810218135	33.20369319785872	36.71526939426285
5	16.280177292855857	35.227006912646836	33.04200152180401	15.450814272693297
6	37.223668892013755	32.64669545367774	14.74462730589605	15.385008348412457
7	30.699908853081386	29.917833338262618	20.200637490690376	19.181620317965624
8	27.796785390681507	34.89048254092873	19.01589559241464	18.296836475975123
9	29.00838067685325	14.06495763953679	17.42821844071552	39.49844324289444
10	16.92522995103878	26.25767287666666	32.45428538395976	24.362811788334806
11	37.89886859518131	20.633249266657167	20.260158579506193	21.207723558655328
12	22.44768462628419	23.19063956098912	29.2550688710419	25.106606941684795
13	31.948540977127234	18.806882802270337	23.17867484748351	26.065901373118916
14	23.476717205258023	19.03444762009749	24.132860749555693	33.355974425088796
15	26.892138779921865	26.181616284916124	21.206480035060643	25.719764900101367
16	25.578036819053203	25.096557926689915	23.60738800893724	25.718017245319647
17	25.505273884391293	24.51270679461292	23.939240764988828	26.042778556006958
18	25.31030954999234	25.142635517184825	24.529544776259858	25.017510156562984
19	26.95209678243311	24.316835024077303	24.311188754782524	24.41987943870706
20	25.685383153145647	23.89215491211985	24.34123497352975	26.081226961204752
21	25.35642074923305	24.63746245903094	25.28070024494054	24.725416546795472
22	26.330435811328567	23.522223447074293	23.731337063456	26.41600367814114
23	25.472270096013467	24.06732369524127	24.74017078620267	25.720235422542597
24	24.955333976828786	23.800134166113242	24.78567702801894	26.45885482903903
25	25.881053271206444	23.485421870420808	24.21523578551705	26.418289072855693
26	25.323753048313247	23.968984505023837	24.828326526442016	25.878935920220904
27	26.302103638617258	24.4827277933573	23.94653386282792	25.268634705197524
28	25.454591895721475	25.10902677138256	23.237590978874888	26.198790354021085
29	25.51444907199531	25.024803254402073	24.417056304059667	25.043691369542948
30	26.91072441635052	23.92089038978079	23.579425532429752	25.58895966143894
31	25.85352770839439	24.066853172800037	24.543929319463228	25.535689799342343
32	25.387912144049775	24.370911496073155	24.288032328924764	25.953144030952313
33	25.32919766513321	23.697728317653734	24.379212856286316	26.59386116092674
34	25.883708362124825	24.092026123405937	25.157927496524856	24.866338017944383
35	25.860988849962492	23.793513243190194	24.409729597474772	25.935768309372538
36	25.310107897517526	23.66805179511033	25.177790265293996	25.84405004207815
37	24.71956862502588	23.6201593323421	25.762279963541236	25.897992079090788
38	25.70161617736814	22.844402261734157	25.233513565834155	26.220467995063544
39	24.266589949102915	23.562453115799606	26.409517190201303	25.761439744896176
40	25.800862803722236	22.492955606879843	26.23233188233175	25.473849707066172
41	24.53982905247535	23.196991613945748	26.30099455000578	25.96218478357312
42	24.313373323259672	23.26790606758853	26.677950242924016	25.740770366227782
43	23.794521505564266	22.812608388205213	26.233373753451616	27.159496352778906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	120.5
2	155.0
3	329.5
4	504.0
5	504.0
6	623.0
7	742.0
8	693.0
9	644.0
10	886.0
11	1128.0
12	1128.0
13	1841.0
14	2554.0
15	3535.5
16	4517.0
17	4598.0
18	4679.0
19	4679.0
20	5770.5
21	6862.0
22	7664.0
23	8466.0
24	11092.0
25	13718.0
26	13718.0
27	17409.5
28	21101.0
29	25275.5
30	29450.0
31	36303.0
32	43156.0
33	43156.0
34	52469.0
35	61782.0
36	73002.5
37	84223.0
38	97874.5
39	111526.0
40	111526.0
41	127063.0
42	142600.0
43	160607.0
44	178614.0
45	202664.5
46	226715.0
47	226715.0
48	251639.5
49	276564.0
50	293498.5
51	310433.0
52	310804.5
53	311176.0
54	311176.0
55	290988.5
56	270801.0
57	251231.0
58	231661.0
59	216135.0
60	200609.0
61	200609.0
62	175674.0
63	150739.0
64	126331.5
65	101924.0
66	84978.5
67	68033.0
68	68033.0
69	55773.5
70	43514.0
71	35465.5
72	27417.0
73	22150.0
74	16883.0
75	16883.0
76	13669.5
77	10456.0
78	8240.0
79	6024.0
80	4702.5
81	3381.0
82	3381.0
83	2445.5
84	1510.0
85	1111.5
86	713.0
87	478.5
88	244.0
89	244.0
90	162.5
91	81.0
92	54.0
93	27.0
94	14.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2975416.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.86482852351992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30791770683884	48.67484551124377
2	9.757113128018162	11.682158085867847
3	3.345704057884993	6.008699991471896
4	1.6248241745546903	3.890792823623453
5	0.9295357982992976	2.7823250585830324
6	0.6077095369934906	2.18282563345338
7	0.4462463406643959	1.870012246418562
8	0.3229628862348997	1.5467294243130674
9	0.23724320556352696	1.2782271437487658
>10	1.338580141463926	14.020757877911668
>50	0.05600536609314899	2.2630182170463593
>100	0.023914711618864798	2.612394701868482
>500	0.0016822093291023242	0.7060775363829359
>1k	5.607364427230469E-4	0.4811357480668903
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.360874580226765E-5
2	0.0	0.0	0.0	0.0	3.360874580226765E-5
3	0.0	0.0	0.0	0.0	3.360874580226765E-5
4	0.0	0.0	0.0	0.0	3.360874580226765E-5
5	0.0	0.0	0.0	0.0	3.360874580226765E-5
6	0.0	0.0	0.0	0.0	6.72174916045353E-5
7	0.0	0.0	0.0	3.360874580226765E-5	6.72174916045353E-5
8	0.0	0.0	0.0	3.360874580226765E-5	6.72174916045353E-5
9	0.0	0.0	0.0	1.0082623740680294E-4	1.0082623740680294E-4
10	0.0	0.0	0.0	1.0082623740680294E-4	1.0082623740680294E-4
11	0.0	0.0	0.0	1.0082623740680294E-4	1.0082623740680294E-4
12	0.0	0.0	0.0	1.0082623740680294E-4	1.0082623740680294E-4
13	0.0	3.360874580226765E-5	0.0	1.0082623740680294E-4	1.0082623740680294E-4
14	0.0	3.360874580226765E-5	0.0	1.0082623740680294E-4	2.0165247481360589E-4
15	0.0	3.360874580226765E-5	0.0	1.0082623740680294E-4	3.360874580226765E-4
16	0.0	3.360874580226765E-5	0.0	2.0165247481360589E-4	3.6969620382494415E-4
17	0.0	3.360874580226765E-5	0.0	2.3526122061587353E-4	3.6969620382494415E-4
18	0.0	3.360874580226765E-5	0.0	3.0247871222040886E-4	4.0330494962721177E-4
19	0.0	3.360874580226765E-5	0.0	4.0330494962721177E-4	4.0330494962721177E-4
20	0.0	3.360874580226765E-5	0.0	5.041311870340147E-4	4.0330494962721177E-4
21	0.0	3.360874580226765E-5	0.0	7.73001153452156E-4	4.0330494962721177E-4
22	0.0	3.360874580226765E-5	0.0	0.001243523594683903	4.0330494962721177E-4
23	0.0	3.360874580226765E-5	0.0	0.0016468285443111148	5.041311870340147E-4
24	0.0	3.360874580226765E-5	0.0	0.002755917155785947	5.041311870340147E-4
25	0.0	3.360874580226765E-5	0.0	0.0035961358008426386	5.041311870340147E-4
26	0.0	3.360874580226765E-5	0.0	0.005679878040583233	5.041311870340147E-4
27	0.0	3.360874580226765E-5	0.0	0.013645150795720666	5.041311870340147E-4
28	0.0	3.360874580226765E-5	0.0	0.04402745700097062	5.041311870340147E-4
29	0.0	3.360874580226765E-5	0.0	0.08845821895156845	5.041311870340147E-4
30	0.0	3.360874580226765E-5	0.0	0.14865148268342981	5.041311870340147E-4
31	0.0	3.360874580226765E-5	0.0	0.4351996493935638	5.041311870340147E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1375	0.0	20.72	37
GTACTTA	1085	0.0	16.539171	30
TCTTATA	2465	0.0	16.436106	37
TACTTAC	1030	0.0	16.344662	31
CTTACAT	835	0.0	14.401197	33
CCTATAC	245	0.0	14.34694	3
AGTGTAC	1265	0.0	14.332016	27
ACTTACA	1015	0.0	14.216747	32
TAACGTT	80	0.006301723	13.875	4
TTACATA	765	0.0	13.784313	34
CGCGACA	110	2.4584177E-4	13.454545	28
TATACTG	330	0.0	13.454545	5
CTCTTAT	3960	0.0	13.22096	37
CTATACT	310	0.0	13.129032	4
GTAAGAC	325	0.0	13.092307	3
CCCTATA	170	3.7369318E-7	13.058824	2
ATTATAC	255	1.6370905E-11	13.058824	3
ACATATA	540	0.0	13.018518	36
GTCTTAG	400	0.0	12.950001	1
GTCCTAC	300	0.0	12.95	1
>>END_MODULE