FastQCFastQC Report
Thu 9 Feb 2017
SRR2727498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855181
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.22229212295408807No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG13040.15248233999586053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12300.14382920106971508No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT11770.13763168264963793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10370.1212608792758492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT705.099404E-621.1428574
CTTAATA759.2612245E-619.7333342
CTTATAC6850.019.71532837
TATACTG1103.848254E-818.55
TTTATAC852.721796E-517.4117643
CTCGTTA1757.2759576E-1216.91428622
TCGTTAC1554.0017767E-1016.70967723
GGTTAAT904.443816E-516.4444451
CGTTACG1251.6561353E-716.27999924
TAATAGT803.381299E-416.18754
CCCCTAT700.002591766215.8571421
CCCTATA700.002591766215.8571422
TACTATA700.002591766215.8571422
TAATACT1201.9339932E-615.4166664
TCTTATA11250.015.29333237
CATATAC750.00410326414.83
GTATTAT750.00410326414.81
TAGGACA1508.0914106E-814.84
AATACGA750.00410326414.85
TCTTATC1001.092844E-414.7999992
Produced by FastQC (version 0.11.5)