##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727498.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855181
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244244200935242	31.0	31.0	34.0	30.0	34.0
2	31.426084068752697	31.0	31.0	34.0	30.0	34.0
3	31.524315905054017	31.0	31.0	34.0	30.0	34.0
4	35.36175616623849	37.0	35.0	37.0	33.0	37.0
5	34.47263094011677	35.0	35.0	37.0	30.0	37.0
6	34.42571104830439	35.0	35.0	37.0	30.0	37.0
7	34.422187817549734	35.0	35.0	37.0	30.0	37.0
8	34.337950679446806	35.0	35.0	37.0	30.0	37.0
9	35.92410261687292	37.0	35.0	39.0	31.0	39.0
10	35.66618996446366	37.0	35.0	39.0	30.0	39.0
11	35.821464695777856	37.0	35.0	39.0	30.0	39.0
12	35.7769021996513	37.0	35.0	39.0	30.0	39.0
13	35.881834371904894	37.0	35.0	39.0	31.0	39.0
14	36.848481198716996	38.0	36.0	40.0	31.0	41.0
15	36.777395662438714	38.0	36.0	40.0	31.0	41.0
16	36.74764874336544	38.0	36.0	40.0	31.0	41.0
17	36.74425530969467	38.0	36.0	40.0	31.0	41.0
18	36.696784657283075	38.0	36.0	40.0	31.0	41.0
19	36.755364069126884	38.0	36.0	40.0	31.0	41.0
20	36.74641859442621	38.0	36.0	40.0	31.0	41.0
21	36.69543172731854	38.0	36.0	40.0	31.0	41.0
22	36.66434707974101	38.0	36.0	40.0	31.0	41.0
23	36.5906574163832	38.0	35.0	40.0	31.0	41.0
24	36.49273077863049	38.0	35.0	40.0	30.0	41.0
25	36.404018564491025	38.0	35.0	40.0	30.0	41.0
26	36.2082354495715	38.0	35.0	40.0	30.0	41.0
27	36.09951928305236	38.0	35.0	40.0	30.0	41.0
28	35.98017729580054	38.0	34.0	40.0	29.0	41.0
29	35.828301844872605	38.0	34.0	40.0	29.0	41.0
30	35.708145994824484	38.0	34.0	40.0	29.0	41.0
31	35.60719894384931	38.0	34.0	40.0	28.0	41.0
32	35.449922297151126	38.0	34.0	40.0	27.0	41.0
33	35.38647958736221	38.0	34.0	40.0	27.0	41.0
34	35.29582860236605	38.0	34.0	40.0	27.0	41.0
35	35.285379352441176	38.0	34.0	40.0	27.0	41.0
36	35.201447412886864	38.0	34.0	40.0	27.0	41.0
37	35.13662955561454	38.0	33.0	40.0	27.0	41.0
38	35.07610903422784	38.0	33.0	40.0	27.0	40.0
39	35.01354800913491	38.0	33.0	40.0	27.0	40.0
40	34.91460988960232	38.0	33.0	40.0	26.0	40.0
41	34.853005387163655	37.0	33.0	40.0	26.0	40.0
42	34.77292526377457	37.0	33.0	40.0	26.0	40.0
43	33.65245486043305	36.0	31.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	2.0
14	6.0
15	11.0
16	22.0
17	53.0
18	97.0
19	179.0
20	395.0
21	690.0
22	1186.0
23	1947.0
24	3091.0
25	4748.0
26	6980.0
27	10004.0
28	14073.0
29	18840.0
30	25361.0
31	33378.0
32	43863.0
33	58180.0
34	77081.0
35	101204.0
36	128456.0
37	147196.0
38	133034.0
39	45097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41581606700804	18.321969267324693	12.942172475768288	26.320042189898977
2	18.711594387620867	21.006079414767168	34.928629144005775	25.353697053606194
3	20.937438974907067	22.400404124974713	29.252403877073974	27.409753023044242
4	16.149680593932747	15.435328895286496	32.98611638939593	35.42887412138483
5	16.385887899754557	35.289605358397814	32.756223536304006	15.56828320554362
6	36.53986699891602	33.44192632904613	14.443141276525088	15.575065395512762
7	30.089536601023642	29.87870404043121	20.424448157758416	19.607311200786732
8	27.388354044348507	34.82140038190746	19.086485784880626	18.70375978886341
9	27.775523544138608	14.742843912575232	18.070092763987976	39.41153977929818
10	17.142920621482467	26.79771884548417	32.34578410886117	23.713576424172192
11	37.11214351113974	21.169319711265803	20.0646412864645	21.653895491129948
12	22.400637993594337	24.262466074433362	29.302451761673844	24.034444170298453
13	32.055202348976415	19.133493377425363	23.276008236852782	25.535296036745436
14	23.08938107839159	19.729975291780335	25.046042884488777	32.13460074533929
15	26.724050230302122	26.45311343446592	21.642786731697733	25.180049603534222
16	25.340600410907165	25.245883619958814	24.133487530709875	25.280028438424146
17	25.07059908954946	25.31756435187405	24.84351265989305	24.768323898683438
18	25.113280112631127	24.89227426708498	25.33440289248709	24.660042727796807
19	25.89217955029403	25.118892959502144	25.24401267100181	23.744914819202016
20	25.443385669232594	24.56170097324426	24.95962842953714	25.035284927986005
21	24.944310034951663	25.459873406916195	25.004998941744493	24.590817616387643
22	25.617500856543817	24.92220945039705	24.240599358498375	25.219690334560752
23	25.17198113615714	24.522177176527542	24.56591060839752	25.739931078917795
24	24.916479669216223	24.744703168101257	24.8695890109813	25.469228151701216
25	25.51471559821839	24.11489497544964	24.444065057572608	25.926324368759364
26	25.13269120806005	24.670800684299582	25.09363514858258	25.10287295905779
27	25.766007430006045	24.824335433083757	24.733360540049418	24.67629659686078
28	25.290435591997486	25.462796764661515	24.35273936160883	24.894028281732172
29	25.265879386936803	25.201097779300525	25.03376478195844	24.499258051804237
30	25.430873698082628	24.944310034951663	24.72844929903728	24.896366967928426
31	25.188585808150556	24.684131195618235	24.934019815688142	25.19326318054307
32	24.583684623489063	24.860000397576655	24.957523611960507	25.598791366973778
33	25.014236752219706	24.585087835206814	24.81240813348285	25.588267279090626
34	25.318733694972174	24.676179662550968	25.24494814548031	24.760138496996543
35	25.127429164118475	24.377529435289137	25.223899969714015	25.271141430878373
36	24.856960105521523	24.313683302131363	25.533658956408058	25.295697635939057
37	24.116648990096834	23.91856226927399	25.941642763344834	26.02314597728434
38	24.575265353182544	23.56518678501978	26.203575617325452	25.655972244472224
39	23.88441745080866	23.5635497046824	26.897346877444654	25.65468596706428
40	24.62192214279784	23.177198745060988	27.154134621793514	25.046744490347656
41	24.08402431765907	23.17065042371147	27.235988638662455	25.509336619966998
42	23.2212829798604	23.47631670956207	27.772015514844227	25.5303847957333
43	23.292963711775634	22.995482827611934	27.541537990203242	26.170015470409187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	49.5
2	65.0
3	160.5
4	256.0
5	256.0
6	381.0
7	506.0
8	499.5
9	493.0
10	631.5
11	770.0
12	770.0
13	1370.5
14	1971.0
15	3040.5
16	4110.0
17	3634.5
18	3159.0
19	3159.0
20	3506.5
21	3854.0
22	3676.5
23	3499.0
24	4290.5
25	5082.0
26	5082.0
27	6242.5
28	7403.0
29	8913.0
30	10423.0
31	12624.5
32	14826.0
33	14826.0
34	17771.5
35	20717.0
36	23785.0
37	26853.0
38	30882.5
39	34912.0
40	34912.0
41	39450.0
42	43988.0
43	48969.5
44	53951.0
45	59240.5
46	64530.0
47	64530.0
48	70761.0
49	76992.0
50	80485.5
51	83979.0
52	85192.5
53	86406.0
54	86406.0
55	81698.0
56	76990.0
57	69210.5
58	61431.0
59	56924.0
60	52417.0
61	52417.0
62	45640.0
63	38863.0
64	32929.0
65	26995.0
66	22709.5
67	18424.0
68	18424.0
69	15125.5
70	11827.0
71	9878.0
72	7929.0
73	6402.5
74	4876.0
75	4876.0
76	3956.5
77	3037.0
78	2437.0
79	1837.0
80	1412.5
81	988.0
82	988.0
83	728.0
84	468.0
85	341.0
86	214.0
87	150.5
88	87.0
89	87.0
90	49.5
91	12.0
92	8.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	855181.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.5514181803795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.87753861175113	68.92579163722738
2	6.7718077379069666	10.503265874391058
3	1.9026143196562622	4.426513162189231
4	0.8630565589574035	2.677250404680998
5	0.478305958914009	1.8546652718953862
6	0.2991202201250119	1.3918318366273175
7	0.19678329227465371	1.0682576373072459
8	0.13080881476303424	0.8115527276294294
9	0.09320951239372616	0.6505676886631611
>10	0.3645840578682497	4.536590442359141
>50	0.010709700161131323	0.5732894465963068
>100	0.009802355240126338	1.353365921947363
>500	9.048327208980471E-4	0.4494456439222006
>1k	7.540272674150392E-4	0.7776123045638075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1901	0.22229212295408807	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1304	0.15248233999586053	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1230	0.14382920106971508	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	1177	0.13763168264963793	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1037	0.1212608792758492	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1693430981277648E-4
2	0.0	0.0	0.0	0.0	1.1693430981277648E-4
3	0.0	0.0	0.0	0.0	1.1693430981277648E-4
4	0.0	0.0	0.0	1.1693430981277648E-4	1.1693430981277648E-4
5	0.0	0.0	0.0	1.1693430981277648E-4	1.1693430981277648E-4
6	0.0	0.0	0.0	1.1693430981277648E-4	1.1693430981277648E-4
7	0.0	0.0	0.0	1.1693430981277648E-4	1.1693430981277648E-4
8	0.0	0.0	0.0	1.1693430981277648E-4	1.1693430981277648E-4
9	0.0	0.0	0.0	2.3386861962555297E-4	1.1693430981277648E-4
10	0.0	0.0	0.0	2.3386861962555297E-4	1.1693430981277648E-4
11	0.0	1.1693430981277648E-4	0.0	3.508029294383294E-4	1.1693430981277648E-4
12	0.0	1.1693430981277648E-4	0.0	3.508029294383294E-4	1.1693430981277648E-4
13	0.0	1.1693430981277648E-4	0.0	3.508029294383294E-4	1.1693430981277648E-4
14	0.0	1.1693430981277648E-4	0.0	3.508029294383294E-4	2.3386861962555297E-4
15	0.0	1.1693430981277648E-4	0.0	3.508029294383294E-4	2.3386861962555297E-4
16	1.1693430981277648E-4	1.1693430981277648E-4	0.0	3.508029294383294E-4	2.3386861962555297E-4
17	1.1693430981277648E-4	1.1693430981277648E-4	0.0	7.016058588766588E-4	2.3386861962555297E-4
18	1.1693430981277648E-4	1.1693430981277648E-4	0.0	8.185401686894353E-4	3.508029294383294E-4
19	1.1693430981277648E-4	1.1693430981277648E-4	0.0	9.354744785022119E-4	3.508029294383294E-4
20	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.0016370803373788707	3.508029294383294E-4
21	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.0019878832668172	3.508029294383294E-4
22	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.0025725548158810826	3.508029294383294E-4
23	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.003624963604196071	3.508029294383294E-4
24	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.0057297811808260475	3.508029294383294E-4
25	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.007834598757456023	3.508029294383294E-4
26	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.009822482024273224	3.508029294383294E-4
27	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.02128204438592532	3.508029294383294E-4
28	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.06489854194609095	3.508029294383294E-4
29	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.1356437993828207	3.508029294383294E-4
30	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.22942511585266745	3.508029294383294E-4
31	1.1693430981277648E-4	1.1693430981277648E-4	0.0	0.5933246879900278	4.6773723925110593E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACT	70	5.099404E-6	21.142857	4
CTTAATA	75	9.2612245E-6	19.733334	2
CTTATAC	685	0.0	19.715328	37
TATACTG	110	3.848254E-8	18.5	5
TTTATAC	85	2.721796E-5	17.411764	3
CTCGTTA	175	7.2759576E-12	16.914286	22
TCGTTAC	155	4.0017767E-10	16.709677	23
GGTTAAT	90	4.443816E-5	16.444445	1
CGTTACG	125	1.6561353E-7	16.279999	24
TAATAGT	80	3.381299E-4	16.1875	4
CCCCTAT	70	0.0025917662	15.857142	1
CCCTATA	70	0.0025917662	15.857142	2
TACTATA	70	0.0025917662	15.857142	2
TAATACT	120	1.9339932E-6	15.416666	4
TCTTATA	1125	0.0	15.293332	37
CATATAC	75	0.004103264	14.8	3
GTATTAT	75	0.004103264	14.8	1
TAGGACA	150	8.0914106E-8	14.8	4
AATACGA	75	0.004103264	14.8	5
TCTTATC	100	1.092844E-4	14.799999	2
>>END_MODULE