FastQCFastQC Report
Thu 9 Feb 2017
SRR2727497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567745
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14100.018.76241137
TCTTATA25200.016.73809437
CCTATAC1901.8189894E-1216.5526313
CGTATAC1151.2435794E-616.0869563
TATACTG2700.015.0740755
ACGTATA750.0041062414.82
TATACAG3850.013.9350665
CGTTACG5850.013.91452924
TCGTTAC8400.013.87523
GTAAGAC1601.785902E-713.8753
CTCGTTA8900.013.51123522
TACTTAC6650.013.35338331
ATCTCGT9700.013.35051520
CGTGTTA850.00940886113.05882410
TATCGCT850.00940886113.0588247
TACACTA2001.4648322E-812.9499995
TACGGAT13050.012.90038427
TACTCCG1307.004372E-512.8076935
TCCTATA1901.0474287E-712.6578952
GGTATCA27150.012.6058931
Produced by FastQC (version 0.11.5)