##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727497.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567745
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.714455290537025	34.0	31.0	34.0	31.0	34.0
2	32.89488208525379	34.0	31.0	34.0	31.0	34.0
3	32.985175708647084	34.0	31.0	34.0	31.0	34.0
4	36.41288173085723	37.0	37.0	37.0	35.0	37.0
5	36.279911361914834	37.0	37.0	37.0	35.0	37.0
6	36.2932553660897	37.0	37.0	37.0	35.0	37.0
7	36.2743146223632	37.0	37.0	37.0	35.0	37.0
8	36.26185972516741	37.0	37.0	37.0	35.0	37.0
9	37.99016491123535	39.0	38.0	39.0	35.0	39.0
10	37.984618410317225	39.0	38.0	39.0	35.0	39.0
11	38.04477469530658	39.0	38.0	39.0	35.0	39.0
12	38.03160010047727	39.0	38.0	39.0	35.0	39.0
13	38.05303992413577	39.0	38.0	39.0	35.0	39.0
14	39.45592338803113	40.0	39.0	41.0	37.0	41.0
15	39.469688773612646	40.0	39.0	41.0	37.0	41.0
16	39.462193870497266	40.0	39.0	41.0	37.0	41.0
17	39.473109284605755	40.0	39.0	41.0	37.0	41.0
18	39.44836773121941	40.0	39.0	41.0	37.0	41.0
19	39.48099636061992	40.0	39.0	41.0	37.0	41.0
20	39.45873947763504	40.0	39.0	41.0	37.0	41.0
21	39.44223121844264	40.0	39.0	41.0	37.0	41.0
22	39.41427205583109	40.0	39.0	41.0	37.0	41.0
23	39.377329135097135	40.0	39.0	41.0	37.0	41.0
24	39.34514720114342	40.0	39.0	41.0	36.0	41.0
25	39.29048289452418	40.0	39.0	41.0	36.0	41.0
26	39.207196197441725	40.0	39.0	41.0	36.0	41.0
27	39.16646123349476	40.0	39.0	41.0	36.0	41.0
28	39.10990733114075	40.0	39.0	41.0	36.0	41.0
29	39.0336563794302	40.0	39.0	41.0	36.0	41.0
30	38.98183503424211	40.0	38.0	41.0	35.0	41.0
31	38.92143495557386	40.0	38.0	41.0	35.0	41.0
32	38.846505786205405	40.0	38.0	41.0	35.0	41.0
33	38.807138169872786	40.0	38.0	41.0	35.0	41.0
34	38.76836835433425	40.0	38.0	41.0	35.0	41.0
35	38.762916488981574	40.0	38.0	41.0	35.0	41.0
36	38.71250533055268	40.0	38.0	41.0	35.0	41.0
37	38.696457397444064	40.0	38.0	41.0	35.0	41.0
38	38.6573121552179	40.0	38.0	41.0	35.0	41.0
39	38.61571651390617	40.0	38.0	41.0	35.0	41.0
40	38.56654379621029	40.0	38.0	41.0	35.0	41.0
41	38.52190384948661	40.0	38.0	41.0	35.0	41.0
42	38.4853157147614	40.0	38.0	41.0	35.0	41.0
43	37.46280023912032	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	3.0
13	3.0
14	2.0
15	4.0
16	2.0
17	10.0
18	32.0
19	42.0
20	83.0
21	170.0
22	361.0
23	695.0
24	1233.0
25	1983.0
26	3165.0
27	4822.0
28	7211.0
29	10442.0
30	15063.0
31	20897.0
32	29620.0
33	41429.0
34	61308.0
35	92958.0
36	146351.0
37	259543.0
38	634908.0
39	1235403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.673223003063	16.478544403747257	11.894989572562697	27.95324302062705
2	18.348862523342465	19.231154183924026	35.71441089360509	26.705572399128418
3	20.054950939442975	21.175194577343152	29.19078802606957	29.579066457144304
4	15.35537991506166	14.338768063028065	33.030966860026986	37.27488516188329
5	16.316923993620865	34.94135905239811	33.14250441535277	15.599212538628251
6	37.361614957871595	32.59299502092303	14.707807823596191	15.337582197609187
7	30.97355851145655	29.660266108978888	20.062350428099364	19.303824951465195
8	27.886608678042407	34.682299060070214	18.873992549883265	18.557099712004113
9	28.751219455202907	14.022303616597442	17.39545788230529	39.83101904589436
10	16.97785410934497	26.126348216041702	32.405398511144995	24.490399163468336
11	38.02437547342123	20.513952904201936	20.140084003668587	21.321587618708243
12	22.582772043174067	23.153856788738757	29.316579333228184	24.946791834858992
13	32.038189150402395	18.73055930398073	23.068490056450308	26.162761489166563
14	23.508448074088353	18.852534032779737	24.116919709706377	33.522098183425534
15	26.783228085343364	26.049081976598142	21.117634344531876	26.050055593526615
16	25.71676704657199	24.920387343758822	23.424249682114073	25.938595927555113
17	25.559781053025127	24.49195695055389	23.945718909003816	26.00254308741717
18	25.44493320014254	24.867695195589903	24.467149191216418	25.22022241305114
19	26.94757462286948	24.275852937110187	24.17654401040602	24.600028429614312
20	25.87889373750119	23.826353473573118	24.276709720007243	26.018043068918452
21	25.5135926659384	24.549750851428005	24.926229045329656	25.01042743730394
22	26.420341583763186	23.576094978278604	23.665200399572388	26.338363038385822
23	25.65422189508693	23.996931159441456	24.495968252299196	25.852878693172414
24	25.27112310607167	23.771753036224393	24.532186802038364	26.424937055665577
25	25.96464991656103	23.368519849128322	24.139858124541185	26.526972109769464
26	25.534544902239126	23.885276770084257	24.643841191395563	25.936337136281058
27	26.27020985339276	24.2411142851023	24.00203291214665	25.48664294935829
28	25.635917896831657	24.91657076539921	23.30523474877762	26.142276588991507
29	25.641370151631097	24.776837263824874	24.325663179170828	25.256129405373194
30	26.676363891274253	23.986104539196845	23.701380004634416	25.636151564894487
31	25.911957768392114	24.0465466781164	24.37189051093469	25.669605042556796
32	25.39629129839606	24.317874243743052	24.24773488021591	26.038099577644974
33	25.428810103807038	23.66679713133508	24.349769934319802	26.554622830538076
34	25.881580920223772	23.91436844390701	25.018216372731715	25.1858342631375
35	25.76603206315269	23.722488019643695	24.46601979557939	26.045460121624224
36	25.379077751100677	23.600708014230385	25.113280329627745	25.906933905041196
37	24.81839123433207	23.423899180019824	25.536803693513182	26.220905892134926
38	25.541554944124123	22.82703305818919	25.277626867153863	26.35378513053282
39	24.361297558752916	23.305156859423345	26.413993601389546	25.919551980434196
40	25.685689194215154	22.418659173710783	26.240339286027236	25.65531234604682
41	24.518828777779724	22.993755221020777	26.440631760552545	26.046784240646947
42	24.15013951930585	23.010384598159085	26.864544571209372	25.974931311325694
43	23.737131218247917	22.61673180163918	26.48448346701094	27.161653513101964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	70.0
2	111.0
3	267.0
4	423.0
5	423.0
6	552.5
7	682.0
8	591.5
9	501.0
10	731.5
11	962.0
12	962.0
13	1660.0
14	2358.0
15	3368.5
16	4379.0
17	4060.0
18	3741.0
19	3741.0
20	4533.5
21	5326.0
22	5787.5
23	6249.0
24	8184.0
25	10119.0
26	10119.0
27	12975.5
28	15832.0
29	19330.0
30	22828.0
31	28792.5
32	34757.0
33	34757.0
34	42484.5
35	50212.0
36	59982.5
37	69753.0
38	82054.0
39	94355.0
40	94355.0
41	108122.0
42	121889.0
43	137938.5
44	153988.0
45	174900.0
46	195812.0
47	195812.0
48	217360.0
49	238908.0
50	253183.5
51	267459.0
52	268423.0
53	269387.0
54	269387.0
55	254638.5
56	239890.0
57	222722.5
58	205555.0
59	190701.0
60	175847.0
61	175847.0
62	153979.0
63	132111.0
64	110995.5
65	89880.0
66	74796.0
67	59712.0
68	59712.0
69	48980.5
70	38249.0
71	30994.5
72	23740.0
73	19121.0
74	14502.0
75	14502.0
76	11535.5
77	8569.0
78	6802.0
79	5035.0
80	3836.0
81	2637.0
82	2637.0
83	1894.0
84	1151.0
85	834.0
86	517.0
87	353.0
88	189.0
89	189.0
90	130.0
91	71.0
92	43.0
93	15.0
94	13.0
95	11.0
96	11.0
97	6.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2567745.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.77601080970252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.89134657327584	51.408120577050596
2	9.748963465787016	12.240020718232811
3	3.221991376916866	6.067912965183045
4	1.5611683826170528	3.9201569305173565
5	0.8734773786938974	2.7416712683459363
6	0.5807833856626953	2.1875558457874194
7	0.4008795995517533	1.7615935452395057
8	0.2884797680039778	1.4487687227678547
9	0.22627574005598966	1.2784219473355423
>10	1.1459924818533167	12.380276136948362
>50	0.041779389337086734	1.7857110929303657
>100	0.0172481069740546	1.945039602553556
>500	0.0013038991030393005	0.552511693259412
>1k	3.1045216738083317E-4	0.2822389538481469
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.894467713889035E-5
2	0.0	0.0	0.0	0.0	3.894467713889035E-5
3	0.0	0.0	0.0	0.0	3.894467713889035E-5
4	0.0	0.0	0.0	3.894467713889035E-5	3.894467713889035E-5
5	0.0	0.0	0.0	7.78893542777807E-5	7.78893542777807E-5
6	0.0	0.0	0.0	7.78893542777807E-5	1.1683403141667105E-4
7	0.0	0.0	0.0	7.78893542777807E-5	1.1683403141667105E-4
8	0.0	0.0	0.0	7.78893542777807E-5	1.1683403141667105E-4
9	0.0	0.0	0.0	7.78893542777807E-5	1.557787085555614E-4
10	0.0	0.0	0.0	7.78893542777807E-5	1.557787085555614E-4
11	0.0	0.0	0.0	7.78893542777807E-5	1.557787085555614E-4
12	0.0	0.0	0.0	1.1683403141667105E-4	1.557787085555614E-4
13	0.0	0.0	0.0	1.1683403141667105E-4	1.9472338569445174E-4
14	0.0	0.0	0.0	1.557787085555614E-4	2.336680628333421E-4
15	0.0	0.0	0.0	1.9472338569445174E-4	3.115574171111228E-4
16	0.0	0.0	0.0	3.5050209425001316E-4	3.5050209425001316E-4
17	0.0	0.0	0.0	3.894467713889035E-4	3.5050209425001316E-4
18	0.0	0.0	0.0	4.673361256666842E-4	3.5050209425001316E-4
19	0.0	0.0	0.0	6.231148342222456E-4	3.5050209425001316E-4
20	0.0	0.0	0.0	7.399488656389166E-4	3.5050209425001316E-4
21	0.0	0.0	0.0	0.0013241190227222719	3.5050209425001316E-4
22	3.894467713889035E-5	0.0	0.0	0.0018693445026667368	3.5050209425001316E-4
23	3.894467713889035E-5	0.0	0.0	0.0024145699826112017	3.894467713889035E-4
24	3.894467713889035E-5	0.0	0.0	0.0031934635253890085	3.894467713889035E-4
25	3.894467713889035E-5	0.0	0.0	0.004128135776722377	3.894467713889035E-4
26	3.894467713889035E-5	0.0	0.0	0.006270093019361346	4.283914485277938E-4
27	3.894467713889035E-5	0.0	0.0	0.015188424084167236	4.673361256666842E-4
28	3.894467713889035E-5	0.0	0.0	0.04646099982669619	4.673361256666842E-4
29	3.894467713889035E-5	0.0	0.0	0.0988805352556426	4.673361256666842E-4
30	3.894467713889035E-5	0.0	0.0	0.16524226510031176	4.673361256666842E-4
31	3.894467713889035E-5	0.0	0.0	0.4736451633631844	5.062808028055745E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1410	0.0	18.762411	37
TCTTATA	2520	0.0	16.738094	37
CCTATAC	190	1.8189894E-12	16.552631	3
CGTATAC	115	1.2435794E-6	16.086956	3
TATACTG	270	0.0	15.074075	5
ACGTATA	75	0.00410624	14.8	2
TATACAG	385	0.0	13.935066	5
CGTTACG	585	0.0	13.914529	24
TCGTTAC	840	0.0	13.875	23
GTAAGAC	160	1.785902E-7	13.875	3
CTCGTTA	890	0.0	13.511235	22
TACTTAC	665	0.0	13.353383	31
ATCTCGT	970	0.0	13.350515	20
CGTGTTA	85	0.009408861	13.058824	10
TATCGCT	85	0.009408861	13.058824	7
TACACTA	200	1.4648322E-8	12.949999	5
TACGGAT	1305	0.0	12.900384	27
TACTCCG	130	7.004372E-5	12.807693	5
TCCTATA	190	1.0474287E-7	12.657895	2
GGTATCA	2715	0.0	12.605893	1
>>END_MODULE