##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727496.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	981429
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.977367695472623	31.0	31.0	33.0	28.0	34.0
2	31.15667256622741	31.0	31.0	34.0	28.0	34.0
3	31.24398504629474	31.0	31.0	34.0	28.0	34.0
4	35.108419457749875	37.0	35.0	37.0	32.0	37.0
5	34.00038820943746	35.0	35.0	37.0	30.0	37.0
6	33.932052140297465	35.0	33.0	37.0	29.0	37.0
7	33.93572331773363	35.0	33.0	37.0	29.0	37.0
8	33.832442285687506	35.0	33.0	37.0	29.0	37.0
9	35.398626900162924	37.0	34.0	39.0	30.0	39.0
10	35.04632836404875	37.0	34.0	39.0	29.0	39.0
11	35.25395723990223	37.0	34.0	39.0	30.0	39.0
12	35.178247229295245	37.0	34.0	39.0	30.0	39.0
13	35.33903624205113	37.0	34.0	39.0	30.0	39.0
14	36.24567951425931	38.0	35.0	40.0	30.0	41.0
15	36.17183107489181	38.0	35.0	40.0	30.0	41.0
16	36.11974681816005	38.0	34.0	40.0	30.0	41.0
17	36.110746676529835	38.0	34.0	40.0	30.0	41.0
18	36.03754117720182	38.0	34.0	40.0	30.0	41.0
19	36.09351771753229	38.0	34.0	40.0	30.0	41.0
20	36.05669793739537	38.0	34.0	40.0	29.0	41.0
21	35.99921135405618	38.0	34.0	40.0	29.0	41.0
22	35.97716085422379	38.0	34.0	40.0	29.0	41.0
23	35.89114546238189	38.0	34.0	40.0	29.0	41.0
24	35.78137491351896	38.0	34.0	40.0	29.0	41.0
25	35.66603391585127	38.0	34.0	40.0	29.0	41.0
26	35.453584518085364	38.0	34.0	40.0	27.0	41.0
27	35.3219234402081	38.0	34.0	40.0	27.0	40.0
28	35.18207735862706	38.0	34.0	40.0	27.0	40.0
29	35.01749897343567	38.0	33.0	39.0	27.0	40.0
30	34.92087456148127	37.0	33.0	39.0	26.0	40.0
31	34.7723819043456	37.0	33.0	39.0	26.0	40.0
32	34.632644847462224	37.0	33.0	39.0	25.0	40.0
33	34.541619414139994	37.0	33.0	39.0	25.0	40.0
34	34.46869411847418	37.0	33.0	39.0	25.0	40.0
35	34.46662163029623	37.0	33.0	39.0	25.0	40.0
36	34.36118252058987	37.0	33.0	39.0	25.0	40.0
37	34.30317730574499	37.0	32.0	39.0	25.0	40.0
38	34.26612419237663	37.0	32.0	39.0	25.0	40.0
39	34.15497911718525	37.0	32.0	39.0	24.0	40.0
40	34.070195602534675	37.0	32.0	39.0	24.0	40.0
41	34.00083551637459	37.0	32.0	39.0	24.0	40.0
42	33.955237719692406	37.0	32.0	39.0	24.0	40.0
43	32.75417885552597	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	3.0
14	12.0
15	22.0
16	59.0
17	96.0
18	170.0
19	386.0
20	716.0
21	1279.0
22	2136.0
23	3356.0
24	5153.0
25	7706.0
26	11186.0
27	16043.0
28	21529.0
29	29209.0
30	38306.0
31	49438.0
32	63517.0
33	80580.0
34	101000.0
35	122818.0
36	139666.0
37	141949.0
38	111108.0
39	33980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.595643699136666	17.857939800026287	12.39661758517427	27.14979891566277
2	20.21246570052444	20.62665765939258	33.236331920087956	25.92454471999503
3	21.414590357529683	21.87585653164926	28.839070375951803	27.870482734869256
4	17.17352961854602	15.136907509356254	32.036652676862005	35.65291019523572
5	16.7650436251629	35.14599629723597	32.359447295728984	15.729512781872149
6	37.680871463957146	32.385939278338014	14.189513454360936	15.743675803343898
7	30.82943340781656	29.587468884656964	19.667240319982394	19.91585738754408
8	28.344689223570935	33.83606964946013	18.70018106251191	19.11906006445703
9	28.933626375417887	13.557068315690692	17.077139558745465	40.43216575014596
10	17.33808558744443	26.122113774913924	32.162183917532495	24.377616720109145
11	38.607988963032476	20.272989691562	19.524183613893616	21.594837731511905
12	22.71962617774694	23.269029140161948	28.595242243707897	25.416102438383213
13	32.71668149198771	18.748783661375406	22.40885484329483	26.125680003342065
14	23.82444374478439	19.023485142582906	23.60323568999897	33.54883542263373
15	27.487266017205524	25.98384600414294	20.611985176716807	25.916902801934732
16	25.955927530162647	24.81065874352602	23.18680210183314	26.04661162447818
17	26.009930417788755	24.20603018659526	23.382435204176765	26.401604191439215
18	25.88409350039585	24.61736916272089	24.008053562713147	25.490483774170116
19	27.430206362355303	23.964443683649048	23.90014967970174	24.70520027429391
20	26.366145691639435	23.41228963073233	23.823526714617156	26.398037963011078
21	26.054763003742504	24.28897047060969	24.59525854646643	25.061007979181376
22	26.98330699418909	23.349116441433868	23.12057214531056	26.547004419066482
23	26.20678622702203	23.742726167659605	24.074487303717333	25.976000301601033
24	25.792288591431472	23.42584129875926	24.024152536760173	26.757717573049096
25	26.56096365605663	23.0419113354099	23.62738415106951	26.769740857463965
26	26.088489335448617	23.631867409664885	24.072245674419648	26.20739758046685
27	26.987892145025267	23.898417511608073	23.439902427990205	25.673787915376455
28	26.060061400264306	24.75563693349188	22.760790643031743	26.423511023212072
29	26.2446901406011	24.580993632753874	23.770644641639894	25.403671585005128
30	27.41308846590023	23.70176548685641	22.964778909121293	25.920367138122064
31	26.37246301056928	23.8119109991655	24.04310449354971	25.772521496715505
32	25.982725189494094	23.93601574846474	23.69504059896335	26.38621846307782
33	25.88256511678379	23.53282815160343	23.738039124582624	26.84656760703016
34	26.333947743545384	23.77258059421517	24.558067878572977	25.33540378366647
35	26.358707558060747	23.363177570664817	23.96474936037146	26.31336551090298
36	26.06627682695335	23.228374136081165	24.48857737034467	26.216771666620815
37	25.29902825369945	23.32456041140011	25.21751446105628	26.158896873844164
38	26.160017688493003	22.50585625654021	24.687572916634824	26.646553138331964
39	24.801284657372054	23.319262014878305	25.814602992167547	26.064850335582097
40	26.296043829966305	22.148723952522293	25.705068833303272	25.85016338420813
41	25.10747084098799	22.790339392864894	25.801357000863028	26.300832765284092
42	24.685738856300354	22.80490998329986	26.255592610367128	26.253758550032657
43	24.410935482852043	22.432188166438937	25.736961104674915	27.419915246034098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	25.5
2	30.0
3	63.5
4	97.0
5	97.0
6	126.0
7	155.0
8	151.0
9	147.0
10	188.0
11	229.0
12	229.0
13	453.0
14	677.0
15	950.0
16	1223.0
17	1148.0
18	1073.0
19	1073.0
20	1335.0
21	1597.0
22	1660.0
23	1723.0
24	2300.5
25	2878.0
26	2878.0
27	3852.0
28	4826.0
29	6042.0
30	7258.0
31	9349.0
32	11440.0
33	11440.0
34	14416.5
35	17393.0
36	20754.5
37	24116.0
38	28398.5
39	32681.0
40	32681.0
41	37836.0
42	42991.0
43	49559.0
44	56127.0
45	64258.0
46	72389.0
47	72389.0
48	81492.5
49	90596.0
50	97300.0
51	104004.0
52	104976.5
53	105949.0
54	105949.0
55	100671.0
56	95393.0
57	88646.0
58	81899.0
59	76483.0
60	71067.0
61	71067.0
62	62165.5
63	53264.0
64	44755.5
65	36247.0
66	30212.0
67	24177.0
68	24177.0
69	19839.0
70	15501.0
71	12601.0
72	9701.0
73	7893.5
74	6086.0
75	6086.0
76	4984.5
77	3883.0
78	3049.0
79	2215.0
80	1768.0
81	1321.0
82	1321.0
83	961.0
84	601.0
85	451.5
86	302.0
87	200.0
88	98.0
89	98.0
90	70.5
91	43.0
92	26.5
93	10.0
94	5.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	981429.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.73310477964739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.40843839031676	65.32303984848897
2	7.310962784716652	10.927418956606646
3	2.2093491424141734	4.953345628645876
4	1.0433872204795676	3.1190226589537833
5	0.5972601317075925	2.2317552001804755
6	0.38605895238093413	1.7310830483643151
7	0.24853181702261395	1.3001488025839014
8	0.16622668145738562	0.9938108802022296
9	0.12108063148573815	0.8143858367648588
>10	0.4760786820285554	5.7458175406372884
>50	0.019389569644115826	0.9880970329287437
>100	0.01282663596234349	1.697389835016856
>500	4.0936038358230616E-4	0.1746847306260798
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.0189224080397053E-4	0.0	1.0189224080397053E-4	0.0
5	0.0	1.0189224080397053E-4	0.0	1.0189224080397053E-4	0.0
6	0.0	1.0189224080397053E-4	0.0	1.0189224080397053E-4	0.0
7	0.0	1.0189224080397053E-4	0.0	1.0189224080397053E-4	0.0
8	0.0	1.0189224080397053E-4	0.0	1.0189224080397053E-4	0.0
9	0.0	1.0189224080397053E-4	0.0	2.0378448160794107E-4	1.0189224080397053E-4
10	0.0	1.0189224080397053E-4	0.0	2.0378448160794107E-4	1.0189224080397053E-4
11	0.0	1.0189224080397053E-4	0.0	3.056767224119116E-4	1.0189224080397053E-4
12	0.0	1.0189224080397053E-4	0.0	3.056767224119116E-4	1.0189224080397053E-4
13	0.0	1.0189224080397053E-4	0.0	3.056767224119116E-4	1.0189224080397053E-4
14	0.0	1.0189224080397053E-4	0.0	4.0756896321588213E-4	1.0189224080397053E-4
15	1.0189224080397053E-4	1.0189224080397053E-4	0.0	5.094612040198527E-4	1.0189224080397053E-4
16	1.0189224080397053E-4	1.0189224080397053E-4	0.0	5.094612040198527E-4	1.0189224080397053E-4
17	1.0189224080397053E-4	1.0189224080397053E-4	0.0	5.094612040198527E-4	1.0189224080397053E-4
18	1.0189224080397053E-4	1.0189224080397053E-4	0.0	5.094612040198527E-4	2.0378448160794107E-4
19	1.0189224080397053E-4	1.0189224080397053E-4	0.0	5.094612040198527E-4	2.0378448160794107E-4
20	1.0189224080397053E-4	1.0189224080397053E-4	0.0	5.094612040198527E-4	2.0378448160794107E-4
21	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.001324599130451617	2.0378448160794107E-4
22	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.0019359525752754402	2.0378448160794107E-4
23	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.002241629297687352	2.0378448160794107E-4
24	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.0031586594649230864	2.0378448160794107E-4
25	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.003668120668942939	2.0378448160794107E-4
26	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.00570596548502235	2.0378448160794107E-4
27	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.014366805953359846	2.0378448160794107E-4
28	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.040553111839980276	2.0378448160794107E-4
29	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.083042176255236	2.0378448160794107E-4
30	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.14183399919912698	2.0378448160794107E-4
31	1.0189224080397053E-4	1.0189224080397053E-4	0.0	0.38066941164363394	3.056767224119116E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	50	2.7017127E-4	22.199999	5
TACTTAC	200	0.0	20.349998	31
ACATATA	115	3.0486262E-9	19.304348	36
TGATCGA	70	1.21896715E-4	18.5	10
CTTACAC	130	6.9667294E-10	18.5	3
GATCGAC	50	0.007034168	18.499998	11
CTTATAC	530	0.0	18.150944	37
TACATAT	150	2.5102054E-10	17.266666	35
CTAGTAC	65	0.0015797111	17.076923	3
CATATAA	100	5.8760306E-6	16.65	37
ATAACAC	90	4.444891E-5	16.444445	3
GTACTTA	250	0.0	16.279999	30
TCTAGTA	80	3.3819344E-4	16.1875	2
ATTATAC	70	0.002592132	15.857142	3
TATACTG	105	9.340234E-6	15.857142	5
TTACACC	95	7.0579925E-5	15.578948	4
GGTATCA	1080	0.0	15.416667	1
CGTACCT	85	5.3631596E-4	15.235294	32
GGGTATG	75	0.0041038375	14.8	1
GTCTAGT	100	1.09310524E-4	14.799999	1
>>END_MODULE