FastQCFastQC Report
Thu 9 Feb 2017
SRR2727495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817119
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24270.1335630742950792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20870.11485213681657613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12550.019.6055837
CGTATAC701.2196924E-418.53
TATAACG500.007036416318.55
TAAGCGG957.0632836E-515.5789485
TCGTTAC5250.015.50476223
TCTTATA21350.015.07728337
CGTTACG4750.014.824
CTCGTTA6050.014.06611522
ACTATAC1254.8474096E-513.323
TACTTAC5150.013.29126331
TAGTACT1551.8890369E-613.1290324
TCTATAC1857.389281E-813.03
GGTATCA22850.012.9540491
TAGGAGT2305.7661964E-1012.8695654
TATACTG2501.6007107E-1012.585
AGTGTAC6950.012.51079127
ACTTACA4900.012.45918532
GTACTTA5700.012.33333430
AACCGTC1050.002644588512.3333337
GAATCGT1501.944894E-512.3333336
Produced by FastQC (version 0.11.5)