FastQCFastQC Report
Thu 9 Feb 2017
SRR2727494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603268
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15050.020.40531737
TACTTAC7450.017.13422831
TCGTTAC10850.016.02764923
GTACTTA8350.015.73053830
GTCTTAG4050.015.0740741
TACGGAT16900.014.88757327
CGTTACG9350.014.83957224
ACGGATG17400.014.67241528
GTTACGG13250.014.38113325
TTCGTTA1551.2119017E-714.3225822
CTCGTTA12550.014.29880422
TCTTATA25600.014.01953137
ATACCGT1854.9058144E-914.0000016
GTATAAA3100.013.7258061
CGTTACA2700.013.70370424
TATACTC1907.1377144E-913.6315795
TTACGGA18900.013.507936526
CGGATGG19550.013.342710529
CGACTTA1951.0275471E-813.2820519
ATCTCGT14000.013.21428620
Produced by FastQC (version 0.11.5)